L-Threonine export protein variant and method for production of L-Threonine using same

Patent metadata

Field	Value
Publication number	US-12584152-B2
Application number	US-202017623226-A
Country	US
Kind code	B2
Filing date	Sep 9, 2020
Priority date	Sep 9, 2019
Publication date	Mar 24, 2026
Grant date	Mar 24, 2026

Abstract

The present disclosure relates to an L-threonine export protein variant, a microorganism including same, and a method for production of L-threonine using same.

First claim

The invention claimed is:

1 . An L-threonine exporter protein variant having comprising an amino acid sequence having at least 80% sequence identity to the amino acid sequence of SEQ ID NO: 1,
wherein: the amino acid sequence has a threonine at a position corresponding to the 53 rd position with respect to the amino acid sequence of SEQ ID NO: 1; or the amino acid sequence has any amino acid except for leucine at a position corresponding to the 62 nd position with respect to the amino acid sequence of SEQ ID NO: 1, and the L-threonine exporter protein variant has L-threonine exporting activity.

2 . The L-threonine exporter protein variant of claim 1 , wherein the any amino acid corresponding to the 62 nd position is selected from serine, arginine, alanine, aspartic acid, lysine, proline, cysteine, glycine, threonine, isoleucine, tyrosine, valine, histidine, phenylalanine, methionine, glutamine, asparagine, glutamic acid, or tryptophan.

3 . The L-threonine exporter protein variant of claim 1 , wherein the L-threonine exporter protein variant comprises an amino acid sequence of any one selected from the amino acid sequence of SEQ ID NOS: 93 to 112.

4 . A polynucleotide encoding the L-threonine exporter protein variant of claim 1 .

5 . A vector comprising the polynucleotide of claim 4 .

6 . A microorganism capable of producing L-threonine, the microorganism comprising the L-threonine exporter protein variant of claim 1 , a polynucleotide encoding the L-threonine exporter protein variant, or a vector comprising the polynucleotide encoding the L-threonine exporter variant.

7 . The microorganism of claim 6 , wherein the microorganism is a microorganism of Corynebacterium sp. or Escherichia sp.

8 . A method for producing L-threonine, the method comprising culturing, in a medium, the microorganism of claim 6 .

9 . The method of claim 8 , wherein the method further comprises recovering L-threonine from the cultured medium or microorganism.

Assignees

• Cj Cheiljedang Corp

Inventors

• Seo Chang Il
• Kim Hyo Jin
• Lee Ji Sun
• Choi Sol

Classifications

• C12Y207/02004

  Aspartate kinase (2.7.2.4) · CPC title

• C12Y101/01003

  Homoserine dehydrogenase (1.1.1.3) · CPC title

• C12N2800/101

  for bacteria · CPC title

• C12N15/70

  Vectors or expression systems specially adapted for E. coli · CPC title

• C12N15/52

  Genes encoding for enzymes or proenzymes · CPC title