Magnetic bead based nucleic acid extraction system

Patent metadata

Field	Value
Publication number	US-12584124-B2
Application number	US-202318143675-A
Country	US
Kind code	B2
Filing date	May 5, 2023
Priority date	May 5, 2023
Publication date	Mar 24, 2026
Grant date	Mar 24, 2026

Abstract

A magnetic bead based nucleic acid extraction system includes a plurality of magnetic beads and an alcohol-free buffer system. The alcohol-free buffer system includes a lysis binding buffer, a first wash buffer, a second wash buffer, and an elution buffer that are stable in storage at room temperature. The buffers include a special selection of chaotropic salt, detergent, precipitant and other necessary components for ideal extraction of nucleic acids. The magnetic bead based nucleic acid extraction system is stable, user-friendly and environment-friendly, efficient and efficacious nucleic acid extraction system optimal for pathogens from swab samples, and can achieve efficient both DNA and RNA extractions, be it separately or simultaneously, from both virus and bacteria in swab samples.

First claim

What is claimed is:

1 . A magnetic bead based nucleic acid extraction system, comprising:
a plurality of magnetic beads, and an alcohol-free buffer system, comprising:
a lysis binding buffer comprising 0.5 to 4 M GuSCN, 5 to 20% (v/v) PEG8000, 10 to 1000 mM sodium citrate, and a non-ionic detergent selected from the group consisting of 0.5 to 4% (v/v) nonylphenyl polyethylene glycol, 0.5 to 4% (v/v) secondary alcohol ethoxylate, 0.5 to 4% (v/v) ethylene oxide-propylene oxide copolymer mono(2-ethylhexyl) ether and mixtures thereof;
a first wash buffer comprising 0.5 to 3 M GuSCN, 5 to 20% (v/v) PEG8000, 0.5 to 2 M sodium chloride, 5 to 25 mM EDTA, and a non-ionic detergent selected from the group consisting of 0.25 to 4% (v/v) ethylene oxide-propylene oxide copolymer mono(2-ethylhexyl) ether, 0.1 to 4% (v/v) 2-[4-(2,4,4-trimethylpentan-2-ylphenoxy]ethanol, 0.25 to 4% (v/v) secondary alcohol ethoxylate and mixtures thereof;
a second wash buffer comprising 1 to 25 mM hexammine cobalt (III) chloride, 5 to 20% (v/v) PEG8000, 5 to 1000 mM sodium chloride, and a non-ionic detergent selected from the group consisting of 0.25 to 2% (v/v) ethylene oxide propylene oxide copolymer mono(2-ethylhexyl) ether, 0.1 to 2% (v/v) 2-[4-(2,4,4-trimethylpentan-2-yl phenoxy]ethanol, 0.25 to 2% (v/v) secondary alcohol ethoxylate and mixtures thereof; and
an elution buffer.

2 . The magnetic bead based nucleic acid extraction system according to claim 1 , wherein the pH value of the lysis binding buffer is 3 to 5.

3 . The magnetic bead based nucleic acid extraction system according to claim 1 , wherein the pH value of the first wash buffer is 3 to 5.

4 . The magnetic bead based nucleic acid extraction system according to claim 1 , wherein the first wash buffer further comprises 0.01 to 1% (v/v) lactic acid.

5 . The magnetic bead based nucleic acid extraction system according to claim 1 , wherein the pH value of the second wash buffer is 3 to 5.

6 . The magnetic bead based nucleic acid extraction system according to claim 1 , wherein the second wash buffer further comprises 0.01 to 1% (v/v) lactic acid.

7 . The magnetic bead based nucleic acid extraction system according to claim 1 , wherein the pH value of the elution buffer is 8.

8 . The magnetic bead based nucleic acid extraction system according to claim 1 , wherein the elution buffer comprises 1-25 mM EDTA.

Assignees

• Delta Electronics Intl Singapore Pte Ltd

Inventors

• Boey Jia Hui Esther
• Zhang Weishi
• Krishnamurthy Casthri

Classifications

• C12N15/1013 Primary

  by using magnetic beads · CPC title

• C12Q1/6806 Primary

  Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay (C12Q1/6804 takes precedence) · CPC title