Sensing vessels for cell cultures

Patent metadata

Field	Value
Publication number	US-12584089-B2
Application number	US-202017780448-A
Country	US
Kind code	B2
Filing date	Nov 9, 2020
Priority date	Nov 27, 2019
Publication date	Mar 24, 2026
Grant date	Mar 24, 2026

Abstract

A sensing cell culture vessel having one or more sensors on or in the vessel is configured to collect readings of various parameters or characteristics of a cell culture located within the sensing cell culture vessel and transmit the readings. The sensing cell culture vessels may be accompanied by a sensing plate having means for reading the one or more sensors and transmitting the one or more sensors to a server hosting electronic lab notebook for analyzing and storage of the readings. Sensing plates may further be equipped with cameras for imaging cell cultures located in the sensing cell culture vessels and transmitting the images to the server hosting the electronic lab notebook for analyzing and storage of the images. Embodiments of the invention allow for the continuous and automatic monitoring of cell cultures.

First claim

We claim:

1 . A sensing plate comprising:
a surface on which one or more sensing vessels may be placed, wherein the one or more sensing vessels each comprise a cell culture vessel and one or more sensors disposed on or in the cell culture vessel; one or more cameras for imaging cell cultures disposed within the one or more sensing vessels or for imaging passive sensors located on or in the sensing vessels; and a wired or wireless connection for transmitting images collected from the one or more cameras.

2 . The sensing plate of claim 1 , the images collected from the one or more cameras being transmitted via a wired or wireless connection to a server.

3 . The sensing plate of claim 2 , the server supporting an electronic lab notebook for analyzing the images transmitted via a wired or wireless connection.

4 . The sensing plate of claim 3 , the electronic lab notebook comprising one or more machine learning models trained to detect particular characteristics of the cell cultures based on images of the cell cultures, or to detect readings of passive sensors based on images of the sensors.

5 . The sensing plate of claim 1 , the one or more sensors in the one or more sensing vessels having RFID or proximity tags for transmitting readings collected by the sensors, further comprising:
a sensor reader for reading the RFID or proximity tags; the readings from the RFID or proximity tags being transmitted via the wired or wireless connection to a server.

6 . The sensing plate of claim 1 , the one or more sensors in the one or more sensing vessels having wireless transmitters for transmitting readings collected by the sensors, further comprising:
a sensor reader for establishing a wireless connection to the one or more sensors and receiving readings transmitted by the sensors; the readings transmitted by the sensors being transmitted via a wired or wireless connection to a server.

7 . The sensing plate of claim 1 , the one or more cameras embedded in the surface of the sensing plate, such that a sensing vessel may be placed over each of the cameras.

8 . The sensing plate of claim 1 , further comprising one or more lights for illuminating the sensing plate.

9 . A system comprising:
an electronic lab notebook; one or more sensing vessels, each sensing vessel comprising a cell culture vessel and one or more sensors disposed on or in the cell culture vessel, each of the one or more sensors capable of sensing a particular parameter or characteristic of the of a cell culture disposed within the cell culture vessel; and a sensing plate comprising a surface on which the sensing vessels may be placed, one or more cameras for imaging cell cultures disposed within the sensing vessels or for imaging passive sensors located on or in the sensing vessels, and a wired or wireless connection for transmitting images collected from the one or more cameras; wherein readings from the one or more sensors are transmitted to the electronic lab notebook for storage and analysis.

10 . The system of claim 9 , the electronic lab notebook comprising one or more machine learning models trained to detect various parameters or characteristics of cell cultures contained in the one or more sensing vessels.

11 . The system of claim 9 , the electronic lab notebook configured to detect milestones reached in an experimental protocol or deviations from expected results in an experimental protocol based on the readings from the one or more sensors or based on images received from the one or more cameras and providing a notification or alert to a user.

12 . The system of claim 9 , the sensing plate having a wireless connection to the electronic lab notebook, the sensing plate collecting readings from the one or more sensors and transmitting them to the electronic lab notebook via the wireless connection.

13 . The system of claim 9 , the electronic lab notebook having a protocol data storage for storing experimental protocols and images and readings from one or more sensors collected from cell cultures associated with the experimental protocol.

14 . The system of claim 9 , the one or more sensors being active sensors having means for transmitting readings from the sensors.

15 . The system of claim 14 , the one or more sensors further comprising a proximity tag for transmitting readings from the sensors.

16 . The system of claim 14 , the one or more sensors further comprising a wireless transmitter for transmitting readings from the sensors.

17 . The system of claim 9 , the one or more sensors being passive sensors.

Assignees

• Corning Inc

Inventors

• Coddaire John Wilfred
• De Chambeau Maryanne
• Eickmann James Thomas
• Flaherty Paula Mary
• Frutos Anthony Glenn
• Goral Vasiliy Nikolaevich
• Julien Angela Langer
• Kosovsky Marshall Jay
• Lanterman Brent Ravaughn
• Martin Gregory Roger
• Mccarthy Christie Leigh
• Shyu John
• Sirkka Tora Ann-Beatrice Eline
• Tanner Allison Jean
• Titus Kimberly Ann
• Wood Timothy James

Classifications

• C12M41/48

  Automatic or computerized control (automatic analysis G01N35/00) · CPC title

• C12M23/12

  Well or multiwell plates (C12M25/04 takes precedence) · CPC title

• B01L2300/0627

  Sensor or part of a sensor is integrated · CPC title

• B01L2300/024

  Storing results with means integrated into the container · CPC title

• B01L2300/023

  Sending and receiving of information, e.g. using Bluetooth® · CPC title