Bulk fluid wash module

Patent metadata

Field	Value
Publication number	US-12578253-B2
Application number	US-201917278970-A
Country	US
Kind code	B2
Filing date	Dec 19, 2019
Priority date	Dec 20, 2018
Publication date	Mar 17, 2026
Grant date	Mar 17, 2026

Abstract

A bulk fluid wash module for an automated treatment apparatus for treating tissue samples disposed on slides, the bulk fluid wash module comprising: a body; a cavity disposed in the body, the cavity configured to receive a plurality of bulk fluids dispensed from a plurality of probes of a bulk fluid robot of the automated treatment apparatus, whereby the plurality of probes are configured to dispense the bulk fluids to the slides and to the cavity; and a scavenger port disposed in the body, the scavenger port in fluid communication with the cavity and configured to remove the bulk fluids from the cavity.

First claim

The invention claimed is:

1 . A bulk fluid wash module for an automated treatment apparatus for treating tissue samples disposed on slides, the bulk fluid wash module comprising:
a body; a cavity disposed in the body, the cavity having an opening at a top of the body and being configured to receive a plurality of bulk fluids dispensed from a plurality of probes of a bulk fluid robot of the automated treatment apparatus, whereby the plurality of probes are configured to dispense the plurality of bulk fluids to the slides and to the cavity; a scavenger port disposed in the body, the scavenger port in fluid communication with the cavity inside the body and configured to remove the plurality of bulk fluids from the cavity, wherein the scavenger port comprises:
a top opening at the top of the body that is spaced from the opening of the cavity, the top opening being configured to receive a scavenger probe configured to apply a vacuum force to remove the plurality of bulk fluids from the cavity;
a bottom opening within the body adjacent a bottom of the body; and
a scavenger cavity extending within the body from the top opening of the scavenger port to the bottom opening of the scavenger port; and
a collection point forming a channel that extends within the body between a bottom end of the cavity and the bottom opening of the scavenger port such that the top opening of the scavenger port is fluidly connected to the cavity inside the body via the channel formed by the collection point, the bottom opening of the scavenger port, and the scavenger cavity of the scavenger port.

2 . The bulk fluid wash module of claim 1 , wherein the plurality of bulk fluids are supplied to the plurality of probes via a plurality of lines connected to a plurality of bulk fluid containers and the cavity is configured to receive at least part of the plurality of bulk fluids in each of the lines.

3 . The bulk fluid wash module of claim 2 , wherein the top opening of the scavenger port is chamfered to guide the scavenger probe to be received in the scavenger port.

4 . The bulk fluid wash module of claim 2 , further comprising a bottom scavenger port configured to provide an opening in a side of the body to output the plurality of bulk fluids from the cavity, wherein the bottom scavenger port is connected to the channel formed by the collection point and in fluid communication with the bottom end of the cavity and the bottom opening of the scavenger port.

5 . The bulk fluid wash module of claim 1 , wherein the cavity comprises a flow channel with a slope configured to provide a substantially uniform flow of the plurality of bulk fluids dispensed from the plurality of probes to the scavenger port, an end of the flow channel comprising the bottom end of the cavity.

6 . The bulk fluid wash module of claim 5 , wherein a first one of the plurality of probes is configured to dispense a wash fluid and the wash fluid flows through the flow channel of the cavity to remove the plurality of bulk fluids dispensed from other ones of the plurality of probes from the cavity.

7 . The bulk fluid wash module of claim 1 , wherein the body comprises a top plane at the top of the body such that the opening of the cavity is formed in the top plane, and the plurality of probes are configured to dispense the plurality of bulk fluids to the cavity near the top plane of the cavity.

8 . The bulk fluid wash module of claim 7 , wherein the cavity is configured to receive the plurality of probes below the top plane of the cavity.

9 . The bulk fluid wash module of claim 7 , wherein the cavity comprises a plurality of probe ports configured to receive each of the plurality of probes.

10 . The bulk fluid wash module of claim 1 , further including a liquid level sensor configured to sense an amount of dispensed bulk fluid in the cavity.

11 . The bulk fluid wash module of claim 1 , further including a pressure sensor configured to sense a pressure in the scavenger port.

12 . The bulk fluid wash module of claim 1 , wherein the cavity comprises a spiral flow channel having a constant downward slope.

13 . The bulk fluid wash module of claim 1 , wherein the channel formed by the collection point extends horizontally within the body.

14 . The bulk fluid wash module of claim 5 , wherein the flow channel comprises a rounded bottom.

15 . A method of removing bulk fluids from a plurality of probes of a bulk fluid robot of an automated treatment apparatus for treating tissue samples disposed on slides, whereby the plurality of probes are configured to dispense the bulk fluids to the slides, the method comprising:
locating the plurality of probes near a cavity of a bulk fluid wash module of the automated treatment apparatus, the bulk fluid wash module comprising:
a body;
the cavity disposed in the body, the cavity having an opening at a top of the body and being configured to receive a plurality of the bulk fluids dispensed from the plurality of probes of the bulk fluid robot of the automated treatment apparatus;
a scavenger port disposed in the body, the scavenger port in fluid communication with the cavity inside the body and configured to remove the plurality of the bulk fluids from the cavity, wherein the scavenger port comprises:
a top opening at the top of the body that is spaced from the opening of the cavity, the top opening being configured to receive a scavenger probe configured to apply a vacuum force to remove the plurality of the bulk fluids from the cavity;
a bottom opening within the body adjacent a bottom of the body; and
a scavenger cavity extending within the body from the top opening of the scavenger port to the bottom opening of the scavenger port; and

a collection point forming a channel that extends within the body between a bottom end of the cavity and the bottom opening of the scavenger port such that the top opening of the scavenger port is fluidly connected to the cavity inside the body via the channel formed by the collection point, the bottom opening of the scavenger port, and the scavenger cavity of the scavenger port;
dispensing the bulk fluids from the plurality of probes into the cavity; and removing the bulk fluids from the cavity via the top opening of the scavenger port.

Assignees

• Leica Biosystems Melbourne Pty

Inventors

• Salama Andrew
• Spink Timothy Ian
• Cuce Matthew
• Fraser Daniel

Classifications

• G01N2035/00138

  Slides · CPC title

• G01N2001/317

  spraying liquids onto surfaces · CPC title

• G01N35/1004 Primary

  Cleaning sample transfer devices · CPC title

• G01N35/00029

  provided with flat sample substrates, e.g. slides (G01N35/028 takes precedence) · CPC title

• G01L2019/0053

  Pressure sensors associated with other sensors, e.g. for measuring acceleration, temperature · CPC title