Electroporation with active compensation

Patent metadata

Field	Value
Publication number	US-12571003-B2
Application number	US-202418616672-A
Country	US
Kind code	B2
Filing date	Mar 26, 2024
Priority date	Jul 22, 2020
Publication date	Mar 10, 2026
Grant date	Mar 10, 2026

Abstract

An apparatus for electroporating cells with a cargo includes electrodes defining a path for a fluid including the cells and the cargo to flow, a power source coupled across the electrodes, and a control circuit. In some examples, the control circuit is configured to detect a decrease in an induced current due to an increase in a resistance between the electrodes, and control the power source to increase the induced current to maintain an electric field between the electrodes. A future value of the resistance between the electrodes may be predicted based on previous values of the resistance. In other examples, the control circuit is configured to detect parameters of the fluid flowing between the electrodes, and control the power source to generate or stop generating electrical pulses in response to detecting the parameters. Other example apparatuses, and methods of electroporating cells with a cargo is also disclosed.

First claim

What is claimed is:

1 . An apparatus for electroporating cells with a cargo, the apparatus comprising:
two electrodes spaced apart from each other and defining a path for a fluid including the cells and the cargo to flow therebetween; a DC power source coupled across the electrodes for providing a plurality of electrical pulses to induce a current through the electrodes for generating an electric field between the electrodes; and a control circuit configured to detect a presence of fluid, and in response to detecting the presence of fluid control the DC power source to generate the plurality of electrical pulses, detect an absence of fluid, and in response to detecting the absence of fluid, control the DC power source to stop generating the plurality of electrical pulses, and wherein the control circuit is further configured to detect a decrease in the induced current due to an increase in a resistance between the electrodes, and provide predictive compensation by increasing the induced current based on feedback of at least one of a proportional-integral-derivative (PID) controller, a proportional-integral (PI) controller, a proportional-derivative (PD) controller, and a proportional (P) controller in order to maintain the electric field between the electrodes at a defined value.

2 . The apparatus of claim 1 , further comprising a first sensor and a second sensor coupled to the control circuit, the first sensor in fluid communication with the path for detecting the presence of fluid, and the second sensor in fluid communication with the path for detecting the absence of fluid.

3 . The apparatus of claim 2 , wherein at least one of the first sensor and the second sensor includes a light sensor.

4 . The apparatus of claim 1 , further comprising a feedback gain factor of the PID controller, the PI controller, the PD controller, and/or the P controller, wherein the feedback gain factor is a fixed value or a variable value.

5 . The apparatus of claim 4 , wherein the feedback gain factor is machine learned from previous compensation results.

Assignees

• Nantcell Inc

Inventors

• Chang Chih-Wei
• Van Lew Jon Thomas
• Mehrabi Artin

Classifications

• A61N1/378

  Electrical supply · CPC title

• A61N1/08

  Arrangements or circuits for monitoring, protecting, controlling or indicating {(for external stimulators A61N1/3603; for implantable neurostimulators A61N1/36128; for heart stimulators A61N1/37; for defibrillators A61N1/3925)} · CPC title

• A61N1/0476

  Array electrodes (including any electrode arrangement with more than one electrode for at least one of the polarities) · CPC title

• H03K3/00

  Circuits for generating electric pulses; Monostable, bistable or multistable circuits (H03K4/00 takes precedence; for digital function generators in computers G06F1/02) · CPC title

• C12M35/02 Primary

  Electrical or electromagnetic means, e.g. for electroporation or for cell fusion · CPC title