Method of producing a tripeptide gamma-glu-val-gly using enterobacteriaceae

Patent metadata

Field	Value
Publication number	US-12570693-B2
Application number	US-202017100239-A
Country	US
Kind code	B2
Filing date	Nov 20, 2020
Priority date	May 23, 2018
Publication date	Mar 10, 2026
Grant date	Mar 10, 2026

Abstract

A method for producing γ-Glu-Val-Gly is described, wherein the method includes the steps of cultivating a γ-Glu-Val-Gly-producing bacterium belonging to the family Enterobacteriaceae in a culture medium so that the γ-Glu-Val-Gly accumulates in the culture medium or the cells of the bacterium, or both, and collecting the γ-Glu-Val-Gly from the culture medium or the cells of the bacterium, or both. The bacterium has been modified to overexpress a gene encoding a protein having L-threonine 3-dehydrogenase activity and a gene encoding a protein having 2-amino-3-oxobutanoate coenzyme A ligase activity.

First claim

The invention claimed is:

1 . A method for producing γ-Glu-Val-Gly, the method comprising:
(i) cultivating a γ-Glu-Val-Gly-producing bacterium belonging to the family Enterobacteriaceae in a culture medium so that the γ-Glu-Val-Gly is produced and accumulates in the culture medium or the cells of the bacterium, or both, and
(ii) collecting the γ-Glu-Val-Gly from the culture medium or the cells of the bacterium, or both,
wherein the bacterium has been modified to overexpress a gene encoding a protein having L-threonine 3-dehydrogenase activity and a gene encoding a protein having 2-amino-3-oxobutanoate coenzyme A ligase activity,
wherein the bacterium has been modified to overexpress a tolC gene, wherein the tolC gene is overexpressed by transformation with a DNA comprising the tolC gene, a mutation of a promoter of the tolC gene, a mutation of a Shine-Dalgarno (SD) sequence of the tolC gene, a mutation of a spacer between the SD sequence and start codon of the tolC gene, a mutation of a ribosome-binding site of the tolC gene, and combinations thereof, and
wherein the cultivating step (i) is carried out at a pH from 6.6 to 7.5.

2 . The method according to claim 1 , wherein the gene encoding a protein having L-threonine 3-dehydrogenase activity is a tdh gene, and the gene encoding a protein having 2-amino-3-oxobutanoate coenzyme A ligase activity is a kbl gene.

3 . The method according to claim 1 , wherein the protein having L-threonine 3-dehydrogenase activity is selected from the group consisting of:
(A) a protein comprising the amino acid sequence of SEQ ID NO: 2, (B) a protein comprising the amino acid sequence of SEQ ID NO: 2 having a substitution, deletion, insertion and/or addition of 1 to 50 amino acid residues, and wherein the protein has L-threonine 3-dehydrogenase activity, and (C) a protein comprising an amino acid sequence having an identity of not less than 60% with respect to the entire amino acid sequence of SEQ ID NO: 2, and wherein the protein has L-threonine 3-dehydrogenase activity; and wherein the protein having 2-amino-3-oxobutanoate coenzyme A ligase activity is selected from the group consisting of: (D) a protein comprising the amino acid sequence of SEQ ID NO: 4, (E) a protein comprising the amino acid sequence of SEQ ID NO: 4 having a substitution, deletion, insertion and/or addition of 1 to 50 amino acid residues, and wherein the protein has 2-amino-3-oxobutanoate coenzyme A ligase activity, and (F) a protein comprising an amino acid sequence having an identity of not less than 60% with respect to the entire amino acid sequence of SEQ ID NO: 4, and wherein the protein has 2-amino-3-oxobutanoate coenzyme A ligase activity.

4 . The method according to claim 1 , wherein the protein having L-threonine 3-dehydrogenase activity is encoded by a DNA selected from the group consisting of:
(a) a DNA comprising the nucleotide sequence of SEQ ID NO: 1, (b) a DNA comprising a nucleotide sequence that is able to hybridize under a stringent condition with a nucleotide sequence complementary to the sequence of SEQ ID NO: 1, wherein the stringent condition comprises washing three times, at a salt concentration of 0.1×SSC, 0.1% SDS at 65° C., (c) a DNA encoding a protein comprising the amino acid sequence of SEQ ID NO: 2 having a substitution, deletion, insertion and/or addition of 1 to 50 amino acid residues, and wherein the protein has L-threonine 3-dehydrogenase activity, and (d) a DNA which is a variant nucleotide sequence of SEQ ID NO: 1 due to the degeneracy of the genetic code; and wherein the protein having 2-amino-3-oxobutanoate coenzyme A ligase is encoded by a DNA selected from the group consisting of: (e) a DNA comprising the nucleotide sequence of SEQ ID NO: 3, (f) a DNA comprising a nucleotide sequence that is able to hybridize under a stringent condition with a nucleotide sequence complementary to the sequence of SEQ ID NO: 3, wherein the stringent condition comprises washing three times, at a salt concentration of 0.1×SSC, 0.1% SDS at 65° C., (g) a DNA encoding a protein comprising the amino acid sequence of SEQ ID NO: 4 having a substitution, deletion, insertion and/or addition of 1 to 50 amino acid residues, and wherein the protein has 2-amino-3-oxobutanoate coenzyme A ligase activity, and (h) a DNA which is a variant nucleotide sequence of SEQ ID NO: 3 due to the degeneracy of the genetic code.

5 . The method according to claim 1 , wherein the gene encoding the protein having L-threonine 3-dehydrogenase activity and the gene encoding the protein having 2-amino-3-oxobutanoate coenzyme A ligase activity are each overexpressed by a method selected from the group consisting of:
(i) increasing the copy number of the gene or genes in the bacterium, (ii) modifying an expression regulatory region of the gene or genes in the bacterium, and (iii) combinations thereof, wherein the expression of the genes is increased as compared with a bacterium that has not been modified to overexpress a gene encoding a protein having L-threonine 3-dehydrogenase activity and a gene encoding a protein having 2-amino-3-oxobutanoate coenzyme A ligase activity.

6 . The method according to claim 1 , wherein the bacterium has been modified further by a method selected from the group consisting of:
(i) attenuating expression of a gcvP gene as compared with a bacterium that has not been modified to attenuate expression of a gcvP gene, (ii) attenuating expression of sucAB operon genes as compared with a bacterium that has not been modified to attenuate expression of sucAB operon genes, (iii) overexpressing ilVGMEDA operon genes, or (iv) a combination thereof.

7 . The method according to claim 6 , wherein the ilVGMEDA operon genes comprise an ilvG gene that encodes an enzymatically active acetolactate synthase II.

8 . The method according to claim 1 , wherein the bacterium belongs to the genus Escherichia or Pantoea.

9 . The method according to claim 8 , wherein the bacterium is Escherichia coli or Pantoea ananatis.

10 . The method according to claim 1 , wherein the pH of the cultivating step (i) is 6.6.

Assignees

• Ajinomoto Kk

Inventors

• Stoynova Natalia V
• Sycheva Elena V
• Geraskina Natalia V
• Matrosova Elena V
• Smirnov Sergey V
• Sato Ayako
• Higashiura Eri
• Okamoto Misato
• Ito Takayuki
• Watanabe Erika
• Oda Yuki
• Tagami Uno
• Kashiwagi Tatsuki
• Sugiki Masayuki

Classifications

• C07K5/0819 Primary

  with the first amino acid being acidic · CPC title

• C12P21/02 Primary

  having a known sequence of two or more amino acids, e.g. glutathione · CPC title