Miniature single-photon fluorescence microscope implantation device and implantation method

Patent metadata

Field	Value
Publication number	US-12569312-B2
Application number	US-202318313383-A
Country	US
Kind code	B2
Filing date	May 8, 2023
Priority date	Dec 11, 2021
Publication date	Mar 10, 2026
Grant date	Mar 10, 2026

Abstract

A miniature single-photon fluorescence microscope implantation device and implantation method are disclosed. The implantation device includes: a clamp base defining a clamp slot; a clamp block; and a groove defined in a bottom surface of the clamp slot. An end of the clamp block includes a first protrusion, and a bottom surface of the groove vertically defines a through receiving hole.

First claim

What is claimed is:

1 . A miniature single-photon fluorescence microscope implantation device, comprising:
a clamp base, comprising a clamp slot, the clamp slot matching the fluorescence microscope; a clamp block, matching the clamp slot and operative to be clamped in the clamp slot; wherein a groove is defined in a bottom surface of the clamp slot, and an end of the clamp block comprises a first protrusion matching the groove; wherein heights of the groove and the first protrusion both lie in the range of 0.48 to 0.52 mm; wherein a bottom surface of the groove vertically defines a through receiving hole used to clamp a lens; wherein the bottom surface of the groove is parallel to the bottom surface of the clamp slot outside the groove.

2 . The miniature single-photon fluorescence microscope implantation device as recited in claim 1 , wherein a side wall of the clamp base further defines a screw hole; and wherein when the clamp block is fixed to the groove, a bolt is screwed into the screw hole to abut against the clamp block.

3 . The miniature single-photon fluorescence microscope implantation device as recited in claim 1 , wherein a height of the groove and a height of the first protrusion are each 0.5 mm, and a height of the second protrusion is 0.5 mm.

4 . The miniature single-photon fluorescence microscope implantation device as recited in claim 1 , wherein the clamp block comprises a first part and a second part disposed on the first part, wherein the first part matches the clamp slot and is operative to be clamped in the clamp slot; and wherein a projected area of the second part on a horizontal plane is larger than a projected area of the first part on the horizontal plane.

5 . The miniature single-photon fluorescence microscope implantation device as recited in claim 1 , wherein a sleeve is disposed on a lower surface of the clamp base, and the receiving hole is located in the sleeve.

6 . The miniature single-photon fluorescence microscope implantation device as recited in claim 1 , wherein a plurality of fixing blocks are arranged on a periphery of the clamp base.

7 . A miniature single-photon fluorescence microscope implantation method, comprising:
providing the miniature single-photon fluorescence microscope implantation device as recited in claim 1 ; placing the clamp block in the clamp slot of the clamp base, so that the first protrusion is clamped in the groove; inserting the lens into the receiving hole so that the end of the lens abuts against the first protrusion; fixing the clamp base on a stereotaxic instrument, referring to a brain atlas and determining location parameters of a target brain region, drilling a hole in a surface of a skull above the target brain region of an experimental animal thus penetrating the skull, and using a stereotaxic method to implant the lens in the skull of the experimental animal, keeping a manipulator of the stereotaxic instrument still after an implanted depth of the lens reaches the target brain region, and applying a photocurable resin to the bottom surface of the clamp base and a surface of the skull of the experimental animal, and irradiating the photocurable resin thus fixing the clamp base; and pulling out the clamp block, and placing the fluorescence microscope in the clamp slot of the clamp base.

8 . The miniature single-photon fluorescence microscope implantation method as recited in claim 7 , wherein after the lens is inserted into the receiving hole, an adhesive is applied to an end of the receiving hole.

9 . A miniature single-photon fluorescence microscope implantation device, comprising:
a clamp base, comprising a clamp slot, the clamp slot matching the fluorescence microscope; a clamp block, matching the clamp slot and operative to be clamped in the clamp slot; wherein a bottom surface of the clamp slot vertically defines a through receiving hole used to clamp a lens, and end of the clamp block comprises a second protrusion matching the receiving hole, wherein the second protrusion is operative to be clamped in the receiving hole, and wherein a height of the second protrusion lies in the range of 0.48 to 0.52 mm.

10 . The miniature single-photon fluorescence microscope implantation device as recited in claim 9 , wherein a side wall of the clamp base further defines a screw hole; and wherein when the clamp block is fixed to the groove, a bolt is screwed into the screw hole to abut against the clamp block.

11 . The miniature single-photon fluorescence microscope implantation device as recited in claim 9 , wherein a height of the groove and a height of the first protrusion are each 0.5 mm, and a height of the second protrusion is 0.5 mm.

12 . The miniature single-photon fluorescence microscope implantation device as recited in claim 9 , wherein the clamp block comprises a first part and a second part disposed on the first part, wherein the first part matches the clamp slot and is operative to be clamped in the clamp slot; and wherein a projected area of the second part on a horizontal plane is larger than a projected area of the first part on the horizontal plane.

13 . The miniature single-photon fluorescence microscope implantation device as recited in claim 9 , wherein a sleeve is disposed on a lower surface of the clamp base, and the receiving hole is located in the sleeve.

14 . The miniature single-photon fluorescence microscope implantation device as recited in claim 9 , wherein a plurality of fixing blocks are arranged on a periphery of the clamp base.

15 . A miniature single-photon fluorescence microscope implantation method, comprising:
providing the miniature single-photon fluorescence microscope implantation device as recited in claim 9 ; placing the clamp block in the clamp slot of the clamp base, so that the second protrusion is clamped in the receiving hole; inserting the lens into the receiving hole so that the end of the lens abuts against the second protrusion; fixing the clamp base on a stereotaxic instrument, referring to a brain atlas and determining location parameters of a target brain region, drilling a hole in a surface of a skull above the target brain region of an experimental animal thus penetrating the skull, and using a stereotaxic method to implant the lens in the skull of the experimental animal, keeping a manipulator of the stereotaxic instrument still after an implanted depth of the lens reaches the target brain region, and applying a photocurable resin to the bottom surface of the clamp base and a surface of the skull of the experimental animal, and irradiating the photocurable resin thus fixing the clamp base; and pulling out the clamp block, and placing the fluorescence microscope in the clamp slot of the clamp base.

16 . The miniature single-photon fluorescence microscope implantation method as recited in claim 15 , wherein after the lens is inserted into the receiving hole, an adhesive is applied to an end of the receiving hole.

Assignees

• Shenzhen Inst Of Adv Tech Cas

Inventors

• Zhu Yingjie
• Chen Gaowei
• Jiang Shaolei

Classifications

• G02B21/00

  Microscopes · CPC title

• A61B1/002

  having rod-lens arrangements (A61B1/055 takes precedence) · CPC title

• A61B1/00

  Instruments for performing medical examinations of the interior of cavities or tubes of the body by visual or photographical inspection, e.g. endoscopes (examination of body cavities or body tracts using ultrasonic, sonic or infrasonic waves A61B8/12; endoscopic instruments for taking cell samples or for biopsy A61B10/04); Illuminating arrangements therefor (for the eyes A61B3/00) · CPC title

• A61B2503/40

  Animals · CPC title

• A61B90/11

  with guides for needles or instruments, e.g. arcuate slides or ball joints · CPC title