Variant nucleic acid libraries for antibody optimization

Patent metadata

Field	Value
Publication number	US-12565715-B2
Application number	US-202217937386-A
Country	US
Kind code	B2
Filing date	Sep 30, 2022
Priority date	Feb 26, 2019
Publication date	Mar 3, 2026
Grant date	Mar 3, 2026

Abstract

Provided herein are methods and compositions relating to libraries of optimized antibodies having nucleic acids encoding for an antibody comprising modified sequences. Libraries described herein include variegated libraries comprising nucleic acids each encoding for a predetermined variant of at least one predetermined reference nucleic acid sequence. Further described herein are protein libraries generated when the nucleic acid libraries are translated. Further described herein are cell libraries expressing variegated nucleic acid libraries described herein.

First claim

What is claimed is:

1 . An antibody, wherein the antibody comprises a sequence of SEQ ID NO: 39.

2 . The antibody of claim 1 , wherein the antibody is a monoclonal antibody, a polyclonal antibody, a bi-specific antibody, a multispecific antibody, a grafted antibody, a human antibody, a humanized antibody, a synthetic antibody, a chimeric antibody, a camelized antibody, a single-chain Fvs (scFv), a single chain antibody, a Fab fragment, a F(ab′)2 fragment, a Fd fragment, a Fv fragment, a single-domain antibody, an isolated complementarity determining region (CDR), a diabody, a fragment comprised of only a single monomeric variable domain, disulfide-linked Fvs (sdFv), an intrabody, an anti-idiotypic (anti-Id) antibody, or ab antigen-binding fragments thereof.

3 . The antibody of claim 1 , wherein the antibody binds PD-1.

4 . The antibody of claim 1 , wherein the antibody comprises a binding affinity to PD-1 of less than 1 nM.

5 . A pharmaceutical composition comprising the antibody of claim 1 .

Assignees

• Twist Bioscience Corp

Inventors

• Sato Aaron

Classifications

• C07K2317/565

  Complementarity determining region [CDR] · CPC title

• C07K16/00 Primary

  Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies · CPC title

• A61K2039/505

  comprising antibodies · CPC title

• C07K2317/622

  Single chain antibody (scFv) · CPC title

• C07K2317/92

  Affinity (KD), association rate (Ka), dissociation rate (Kd) or EC50 value · CPC title