RNA TARGETING OF MUTATIONS VIA SUPPESSOR tRNAs AND DEAMINASES
US-2021198673-A1 · Jul 1, 2021 · US
Methods of rescuing stop codons via genetic reassignment with ACE-tRNA
US12565649B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-12565649-B2 |
| Application number | US-202318137942-A |
| Country | US |
| Kind code | B2 |
| Filing date | Apr 21, 2023 |
| Priority date | Nov 2, 2017 |
| Publication date | Mar 3, 2026 |
| Grant date | Mar 3, 2026 |
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- Title
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- Abstract
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Abstract
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In certain embodiments, the present invention provides a modified transfer RNA (tRNA) comprising a T-arm, a D-arm, an anticodon-arm and an acceptor arm, wherein the T-arm comprises nucleotides that interact with the elongation factor 1 alpha protein, and methods of use thereof. In certain embodiments, the present invention provides a modified transfer RNA (tRNA) comprising a T-arm, a D-arm, an anticodon-arm and an acceptor arm, (a) wherein the anticodon-arm comprises a tri-nucleotide anticodon, wherein the anticodon is 5′-UCA-3′ and recognizes TGA stop codons, and wherein the acceptor arm is operably linked to a arginine, tryptophan or glycine; (b) wherein the anticodon-arm comprises a tri-nucleotide anticodon, wherein the anticodon is 5′-UUA-3′ and recognizes TAA stop codons, and wherein the acceptor arm is operably linked to a glutamine or, glutamate; or (c) wherein the anticodon-arm comprises a tri-nucleotide anticodon, wherein the anticodon is 5′-CUA-3′ and recognizes TAG stop codons, and wherein the acceptor arm is operably linked to a tryptophan, glutamate or glutamine.
First claim
Opening claim text (preview).
What is claimed is: 1 . A modified transfer RNA (tRNA) comprising a T-arm, a D-arm, an anticodon-arm and an acceptor arm, wherein the anticodon-arm comprises a tri-nucleotide anticodon, wherein the anticodon is 5′-CUA-3′ and recognizes TAG stop codons, wherein the acceptor arm is operably linked to a glutamine, and wherein the modified tRNA is encoded by a sequence that comprises the sequence as set forth in SEQ ID NO 139. 2 . A modified transfer RNA (tRNA) comprising a T-arm, a D-arm, an anticodon-arm and an acceptor arm, wherein the anticodon-arm comprises a tri-nucleotide anticodon, wherein the anticodon is 5′-CUA-3′ and recognizes TAG stop codons, wherein the acceptor arm is operably linked to a glutamine, and wherein the modified tRNA is encoded by a sequence that comprises the sequence as set forth in SEQ ID NO: 141. 3 . A modified transfer RNA (tRNA) comprising a T-arm, a D-arm, an anticodon-arm and an acceptor arm, wherein the anticodon-arm comprises a tri-nucleotide anticodon, wherein the anticodon is 5′-CUA-3′ and recognizes TAG stop codons, wherein the acceptor arm is operably linked to a glutamine, and wherein the modified tRNA is encoded by a sequence that comprises the sequence as set forth in SEQ ID NO: 130. 4 . A modified transfer RNA (tRNA) comprising a T-arm, a D-arm, an anticodon-arm and an acceptor arm, wherein the anticodon-arm comprises a tri-nucleotide anticodon, wherein the anticodon is 5′-CUA-3′ and recognizes TAG stop codons, wherein the acceptor arm is operably linked to a glutamine, and wherein the modified tRNA is encoded by a sequence that comprises the sequence as set forth in SEQ ID NO: 134. 5 . A modified transfer RNA (tRNA) comprising a T-arm, a D-arm, an anticodon-arm and an acceptor arm, wherein the anticodon-arm comprises a tri-nucleotide anticodon, wherein the anticodon is 5′-CUA-3′ and recognizes TAG stop codons, wherein the acceptor arm is operably linked to a glutamine, and wherein the modified tRNA is encoded by a sequence that comprises the sequence as set forth in SEQ ID NO: 140.
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Classifications
Natural ribonucleic acids, i.e. containing only riboses attached to adenine, guanine, cytosine or uracil and having 3'-5' phosphodiester links · CPC title
Synthetic bilayered vehicles, e.g. liposomes or liposomes with cholesterol as the only non-phosphatidyl surfactant · CPC title
- A61P43/00Primary
Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00 · CPC title
Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy · CPC title
- C12N15/11Primary
DNA or RNA fragments; Modified forms thereof (DNA or RNA not used in recombinant technology, C07H21/00); {Non-coding nucleic acids having a biological activity} · CPC title
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- What does patent US12565649B2 cover?
- In certain embodiments, the present invention provides a modified transfer RNA (tRNA) comprising a T-arm, a D-arm, an anticodon-arm and an acceptor arm, wherein the T-arm comprises nucleotides that interact with the elongation factor 1 alpha protein, and methods of use thereof. In certain embodiments, the present invention provides a modified transfer RNA (tRNA) comprising a T-arm, a D-arm, an …
- Who is the assignee on this patent?
- Univ Iowa Res Found
- What technology area does this patent fall under?
- Primary CPC classification A61P43/00. Mapped technology areas include Human Necessities.
- When was this patent published?
- Publication date Tue Mar 03 2026 00:00:00 GMT+0000 (Coordinated Universal Time) (B2). Legal status and post-grant events are not shown on this page.
- What related patents are in patentsdb?
- We list 12 related publications on this page (citations in our corpus or others sharing the same primary CPC).