Sparger assemblies for a bioprocessing system

Patent metadata

Field	Value
Publication number	US-12565634-B2
Application number	US-202217947537-A
Country	US
Kind code	B2
Filing date	Sep 19, 2022
Priority date	Sep 19, 2022
Publication date	Mar 3, 2026
Grant date	Mar 3, 2026

Abstract

A sparger assembly for a bioprocessing system includes a base and a plurality of spargers connected to the base, each sparger including a plurality of pores, the plurality of spargers each have a generally cylindrical shape. Each of the plurality of spargers includes a sidewall and a top, which define the cylindrical shape, the sidewall and the top each include a plurality of pores. The pores of the sidewall can be arranged around a circumference of the sidewall at an array of heights. Ridges may also be located on the sidewall above a respective array of pores.

First claim

What is claimed is:

1 . A sparger assembly for a bioprocessing system, comprising:
a base; and a plurality of spargers connected to the base, each of the plurality of spargers having a generally cylindrical shape with a sidewall and a top that define the cylindrical shape, a plurality of pores disposed about the sidewall, and at least one ridge extending at least partially around a circumference of the sidewall that separates a portion of pores located on the sidewall below the at least one ridge from a portion of pores located on the sidewall above the at least one ridge.

2 . The sparger assembly of claim 1 , wherein:
the plurality of pores are further disposed about the top.

3 . The sparger assembly of claim 1 , wherein:
the pores of the sidewall include a first array of pores located at a first height from a top surface of the base.

4 . The sparger assembly of claim 3 , wherein:
the pores of the sidewall include a second array of pores located at a second height from the top surface of the base, the second height being different than the first height.

5 . The sparger assembly of claim 4 , wherein:
the second array of pores are radially offset in relation to the first array of pores.

6 . The sparger assembly of claim 4 , wherein:
the at least one ridge includes a first ridge extending at least partially around the circumference of the sidewall, the first ridge located above the first array of pores.

7 . The sparger assembly of claim 6 , wherein:
the at least one ridge includes a second ridge extending at least partially around the circumference of the sidewall, the second ridge located above the second array of pores.

8 . The sparger assembly of claim 1 , wherein:
each of the plurality of spargers includes two bonding surfaces, the two bonding surfaces configured to bond with the base upon application of heat and/or vibratory forces.

9 . An impeller and sparger assembly for a bioprocessing system, comprising:
a sparger assembly comprising: a base; a plurality of spargers connected to the base, each sparger including a generally cylindrical shape with a sidewall, a top and a bottom that define the cylindrical shape, with the bottom having a surface directly on the base, and at least one ridge extending at least partially around a circumference of the sidewall that separates a portion of pores located on the sidewall below the at least one ridge from a portion of pores located on the sidewall above the at least one ridge; and an impeller assembly attached to the base.

10 . The impeller and sparger assembly of claim 9 , wherein:
the impeller assembly includes a magnetic hub connected to the base; and an impeller connected to the magnetic hub.

11 . A bioprocessing apparatus, comprising:
a flexible bag; a sparging assembly comprising: a base; a plurality of spargers connected to the base, each sparger including a generally cylindrical shape with a sidewall, a top and a bottom that define the cylindrical shape, with the bottom having a surface disposed directly on the base, and at least one ridge extending at least partially around a circumference of the sidewall that separates a portion of pores located on the sidewall below the at least one ridge from a portion of pores located on the sidewall above the at least one ridge; wherein the base is bonded to the flexible bag such that the plurality of spargers are located within the flexible bag; and an impeller assembly attached to the base, such that the impeller assembly is located within the flexible bag.

12 . The bioprocessing apparatus of claim 11 , wherein:
the base includes at least one input port, the at least one input port in fluid communication with the plurality of spargers.

13 . The bioprocessing apparatus of claim 12 , wherein:
the at least one input port is connectable to a gas source.

14 . The bioprocessing apparatus of claim 13 , wherein:
gas from the gas source is configured to exit through the plurality of pores and into the flexible bag.

Assignees

• Global Life Sciences Solutions Usa Llc

Inventors

• Diana Rafael

Classifications

• C12M27/02

  Stirrer or mobile mixing elements · CPC title

• C12M23/26

  flexible (flexible containers for laboratory use B01L3/505) · CPC title

• C12M23/14

  Bags · CPC title

• B01F2101/44

  Mixing of ingredients for microbiology, enzymology, in vitro culture or genetic manipulation · CPC title

• C12M29/06 Primary

  Nozzles; Sprayers; Spargers; Diffusers (per se B01F23/231, B01J19/26) · CPC title