Fluidic devices involving signal generation at converging liquid fronts

Patent metadata

Field	Value
Publication number	US-12558685-B2
Application number	US-201917252503-A
Country	US
Kind code	B2
Filing date	Jun 19, 2019
Priority date	Jun 22, 2018
Publication date	Feb 24, 2026
Grant date	Feb 24, 2026

Abstract

Articles and methods involving fluidic devices are generally provided. In some embodiments, a fluidic device comprises a first layer comprising first and second regions that are disconnected from each other in the first layer and a second layer comprising a channel in fluidic communication with the first and second regions. The device may also comprise a third layer comprising a channel in fluidic communication with the first and second regions. One or more portions of a channel and/or one or more reagents may comprise a reagent. In some embodiments, a method comprises flowing two or more fluid samples towards each other through a channel. The fluids may meet at an interface and/or may react at an interface.

First claim

What is claimed is:

1 . A fluidic device, comprising:
a first layer comprising first and second regions; a second layer comprising a first channel; and a third layer comprising a second channel in fluidic communication with the first and second regions, wherein:
the first channel directly fluidically connects the first and second regions;
the second channel directly fluidically connects the first and second regions;
the first layer is disposed on the second layer;
the third layer is disposed on the first layer;
the first and second regions are disconnected from each other in the first layer;
at least one of the first layer, second layer, and third layer comprises a porous material;
the first channel is in fluidic communication with the second channel via the first region and via the second region;
the first channel is positioned on both the first region and the second region; and
the second channel is positioned on both the first region and the second region.

2 . The fluidic device of claim 1 , wherein the fluidic device comprises a fourth layer disposed on the third layer.

3 . The fluidic device of claim 2 , wherein the fourth layer comprises a central region in fluidic communication with an environment external to the fluidic device.

4 . The fluidic device of claim 3 , wherein the central region in fluidic communication with the environment external to the fluidic device is positioned directly above the first channel.

5 . The fluidic device of claim 2 , wherein the fluidic device comprises a fifth layer, and wherein the third layer is disposed on the fifth layer.

6 . The fluidic device of claim 5 , wherein the fifth layer comprises a detection region in fluidic communication with the second channel.

7 . The fluidic device of claim 6 , wherein the detection region is positioned directly below the first channel.

8 . The fluidic device of claim 1 , wherein the first and second regions are directly above the second channel.

9 . The fluidic device of claim 1 , wherein the first channel is directly above the first and second regions.

10 . The fluidic device of claim 1 , wherein the first and second regions are not in fluidic communication with each other through the first layer.

11 . The fluidic device of claim 1 , wherein the first channel and second channel are not in fluidic communication with each other other than through the first and second regions.

12 . The fluidic device of claim 1 , wherein the first layer comprises a first porous material, wherein the second layer comprises a second porous material, wherein the third layer comprises a third porous material, and wherein the first, second, and third porous materials are the same.

13 . The fluidic device of claim 1 , wherein the porous material is absorbent.

14 . A fluidic device, comprising:
a first layer comprising first and second regions; a second layer comprising a single first channel; and a third layer comprising a single second channel in fluidic communication with the first and second regions, wherein:
the first layer is disposed on the second layer;
the third layer is disposed on the first layer;
the first and second regions are disconnected from each other in the first layer;
the first and second regions are in fluidic communication with the first channel;
at least one of the first layer, second layer, and third layer comprises a porous material;
the first channel is in fluidic communication with the second channel via the first region and via the second region;
the first channel is positioned on both the first region and the second region; and
the second channel is positioned on both the first region and the second region.

15 . The fluidic device of claim 14 , wherein the fluidic device comprises a fourth layer disposed on the third layer.

16 . The fluidic device of claim 15 , wherein the fourth layer comprises a central region in fluidic communication with an environment external to the fluidic device.

17 . The fluidic device of claim 16 , wherein the central region in fluidic communication with the environment external to the fluidic device is positioned directly above the first channel.

18 . The fluidic device of claim 15 , wherein the fluidic device comprises a fifth layer, and wherein the third layer is disposed on the fifth layer.

19 . The fluidic device of claim 18 , wherein the fifth layer comprises a detection region in fluidic communication with the second channel.

20 . The fluidic device of claim 14 , wherein the porous material is absorbent.

Assignees

• Tufts College

Inventors

• Wilson Daniel J
• Mace Charles R

Classifications

• C12Q1/46

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• C12Q1/28

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• B01L2400/0406

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• B01L2300/168

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