Crispr DNA targeting enzymes and systems

Patent metadata

Field	Value
Publication number	US-12553045-B2
Application number	US-201817260791-A
Country	US
Kind code	B2
Filing date	Dec 28, 2018
Priority date	Jul 16, 2018
Publication date	Feb 17, 2026
Grant date	Feb 17, 2026

Abstract

The disclosure describes novel systems, methods, and compositions for the manipulation of nucleic acids in a targeted fashion. The disclosure describes non-naturally occurring, engineered CRISPR-Cas systems, components, and methods for targeted modification of nucleic acids such as DNA. Each system includes one or more protein components and one or more nucleic acid components that together target nucleic acids.

First claim

What is claimed is:

1 . A RNA guide or a nucleic acid encoding the RNA guide, wherein the RNA guide comprises a direct repeat sequence and a spacer sequence capable of hybridizing to a target nucleic acid, wherein the direct repeat sequence comprises at least 30 contiguous nucleotides of SEQ ID NO: 325;
wherein the direct repeat sequence comprises 5′-YBVMRAC-3′ at the 3′ end, wherein Y is C, T, or U; B is T, U, C, or G; V is G, C, or A; M is A or C; and R is A or G; and wherein the spacer sequence comprises 15-50 nucleotides which are complementary to a mammalian target nucleic acid, and wherein the spacer sequence is capable of hybridizing to the target nucleic acid.

2 . The RNA guide of claim 1 , wherein the spacer sequence comprises 15 to 24 nucleotides or 16 to 22 nucleotides.

3 . An engineered, non-naturally occurring Clustered Regularly Interspaced Short Palindromic Repeat (CRISPR) Cas system comprising the RNA guide of claim 1 and a CRISPR-Cas effector protein or a nucleic acid encoding the CRISPR-Cas effector protein, wherein the CRISPR-Cas effector protein binds to the RNA guide, and wherein the CRISPR-Cas effector protein comprises the amino acid sequence of SEQ ID NO: 127.

4 . The system of claim 3 , wherein the CRISPR-Cas effector protein is capable of recognizing a protospacer adjacent motif (PAM), and the target nucleic acid comprises a PAM comprising the nucleic acid sequence 5′-TTN-3′ or 5′-YTN-3′, wherein N is any nucleobase, and Y is cytosine or thymine.

5 . The system of claim 3 , wherein the targeting of the target nucleic acid by the CRISPR-Cas effector protein and the RNA guide results in a modification in the target nucleic acid.

6 . The system of claim 5 , wherein the modification in the target nucleic acid is a double stranded cleavage event or a single-stranded cleavage event.

7 . The system of claim 5 , wherein the modification results in cell toxicity.

8 . The system of claim 3 , further comprising a donor template nucleic acid.

9 . The system of claim 8 , wherein the donor template nucleic acid is a DNA or an RNA.

10 . The system of claim 3 , wherein the system further comprises a tracrRNA.

11 . The system of claim 3 , wherein the targeting of the target nucleic acid by the CRISPR-Cas effector protein and RNA guide results in the effector complex binding specifically to the target nucleic acid.

12 . The system of claim 3 , wherein the CRISPR-Cas effector protein is nuclease dead and the targeting of the target nucleic acid by the CRISPR-Cas effector protein and RNA guide does not result in a cleavage event in the target nucleic acid.

13 . The system of claim 3 , wherein the CRISPR-Cas effector protein is operably linked to a functional domain.

14 . The system of claim 13 , where the functional domain is a base editing domain.

15 . The system of claim 14 , where the functional domain comprises a sequence with at least 95% identity to the amino acid sequence set forth in SEQ ID NO: 1010 to SEQ ID NO: 1014.

16 . The RNA guide of claim 1 , wherein the target nucleic acid is a DNA.

17 . The RNA guide of claim 16 , wherein the target nucleic acid is a single-stranded DNA.

18 . A cell comprising the RNA guide of claim 1 .

19 . The cell of claim 18 , wherein the cell is a eukaryotic cell.

20 . An engineered vector comprising the RNA guide of claim 1 .

21 . The engineered vector of claim 20 , further comprising a control element operably linked to the nucleic acids in the system, whereby a coding sequence in the nucleic acid is capable of being transcribed and translated in a cell.

22 . The RNA guide of claim 1 , wherein the direct repeat sequence comprises a RNA transcript according to the sequence of SEQ ID NO: 325.

23 . The RNA guide of claim 1 , wherein the target nucleic acid and the spacer sequence comprise at least 90% sequence complementarity to each other.

Assignees

• Arbor Biotechnologies Inc

Inventors

• Cheng David R
• Scott David A
• Yan Winston X

Classifications

• C07K14/4702

  Regulators; Modulating activity · CPC title

• C12N2310/20

  involving clustered regularly interspaced short palindromic repeats [CRISPR] · CPC title

• C12N15/111 Primary

  General methods applicable to biologically active non-coding nucleic acids · CPC title

• C12N15/113 Primary

  Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; {Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing (when used in plants C12N15/8218)} · CPC title