Glucose production method and ethanol production method

Patent metadata

Field	Value
Publication number	US-12545933-B2
Application number	US-202117913858-A
Country	US
Kind code	B2
Filing date	Mar 24, 2021
Priority date	Mar 30, 2020
Publication date	Feb 10, 2026
Grant date	Feb 10, 2026

Abstract

The present invention can: efficiently and readily produce glucose from microalgae that accumulate starch in their cells; and obtain ethanol. During a preparation step of the glucose production method, microalgae are prepared on which a saccharifying enzyme acts on starch accumulated inside the microalgae cells, without disrupting the cell walls. In a saccharification step, starch inside the cells is saccharified and glucose is generated, by adding a saccharifying enzyme to the microalgae without a disruption treatment. The ethanol production method has a step in which, after the saccharification step, the glucose undergoes alcoholic fermentation and ethanol is generated.

First claim

What is claim is:

1 . A glucose production method, comprising:
a preparatory step of preparing microalgae having cell walls which block starch accumulated within cells thereof from flowing out; and a saccharification step of saccharifying the starch within the cells to generate glucose by adding a saccharifying enzyme that acts on the starch within the cells without crushing the cell walls, to the microalgae in which the cell walls have not been crushed, wherein the microalgae are microalgae of a Honda DREAMO strain (accession number FERM BP-22306), the saccharifying enzyme comprises a-amylase and glucoamylase, in the saccharification step, a mixture of the microalgae and the saccharifying enzyme is maintained at 40° C. to 50° C., and the saccharifying enzyme acts on the starch within the cells to generate glucose having a molecular weight smaller than the starch, and the glucose flows out to an exterior of the cells through the cell walls.

2 . An ethanol production method, comprising a fermentation step of causing glucose obtained by a glucose production method to undergo alcoholic fermentation to thereby generate ethanol,
the glucose production method comprising: a preparatory step of preparing microalgae having cell walls which block starch accumulated within cells thereof from flowing out; and a saccharification step of saccharifying the starch within the cells to generate the glucose by adding a saccharifying enzyme that acts on the starch within the cells without crushing the cell walls, to the microalgae in which the cell walls have not been crushed, wherein the microalgae are microalgae of a Honda DREAMO strain (accession number FERM BP-22306), and wherein the saccharifying enzyme acts on the starch within the cells to generate glucose having a molecular weight smaller than the starch, and the glucose out to an exterior of the cells through the cell walls.

3 . The ethanol production method according to claim 2 , further comprising a distillation step of separating the ethanol and the cells by distilling the ethanol obtained in the fermentation step.

4 . The glucose production method according to claim 1 , wherein the microalgae has a cell wall thickness of 0.08 μm to 0.10 μm.

Assignees

• Honda Motor Co Ltd

Inventors

• Shiobara Nozomi
• Kinoshita Shohei
• Doi Mizuho
• Machida Kenji
• Goto Minoru

Classifications

• C12P19/02

  Monosaccharides (2-ketogulonic acid C12P7/60) · CPC title

• C12N9/2428

  Glucan 1,4-alpha-glucosidase (3.2.1.3), i.e. glucoamylase · CPC title

• C12N9/2414

  Alpha-amylase (3.2.1.1.) · CPC title

• C12N1/125

  Unicellular algae isolates · CPC title

• C12R2001/89

  Algae {; Processes using algae} · CPC title