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Recombinant microorganism including genetic modification that increases activity of nitric oxide reductase and method of reducing concentration of nitric oxide in sample by using the same
US12545932B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-12545932-B2 |
| Application number | US-202117224596-A |
| Country | US |
| Kind code | B2 |
| Filing date | Apr 7, 2021 |
| Priority date | Nov 25, 2020 |
| Publication date | Feb 10, 2026 |
| Grant date | Feb 10, 2026 |
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- Title
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Abstract
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A recombinant microorganism including a genetic modification that increases activity of nitric oxide reductase in the recombinant microorganism, a composition for reducing a concentration of nitric oxide in a sample, the composition including the recombinant microorganism, and a method of reducing a concentration of nitric oxide in a sample, are disclosed.
First claim
Opening claim text (preview).
What is claimed is: 1 . A recombinant bacterium comprising a genetic modification that increases expression of a gene encoding a nitric oxide reductase in the recombinant bacterium, wherein the recombinant bacterium belongs to the genus Escherichia or the genus Paracoccus , wherein the gene originates from Paracoccus versutus and wherein the nitric oxide reductase comprises a polypeptide having 95% or greater sequence identity to a polypeptide encoded by SEQ ID NO:21. 2 . A composition for reducing a concentration of nitric oxide in a sample, the composition comprising the recombinant bacterium according to claim 1 . 3 . A method of reducing a concentration of nitric oxide in a sample containing nitric oxide, the method comprising contacting the sample with the recombinant bacterium according to claim 1 . 4 . The method of claim 3 , wherein the genetic modification increases the copy number of the gene encoding the nitric oxide reductase. 5 . The method of claim 3 , wherein the nitric oxide (NO) is in a form of Fe(II)(L)—NO, which is a complex formed by chelating a chelating agent L with Fe 2+ and NO. 6 . The method of claim 3 , wherein the contacting is performed in a sealed container. 7 . The method of claim 3 , wherein the contacting comprises culturing or incubating the recombinant bacterium in the presence of the nitric oxide-containing sample. 8 . The method of claim 3 , wherein the contacting comprises culturing the recombinant bacterium in a sealed container and under conditions in which the recombinant bacterium grows. 9 . The recombinant bacterium of claim 1 , wherein the genetic modification increases the copy number of the gene encoding the nitric oxide reductase. 10 . The method of claim 3 , wherein the sample is in a liquid state or gas state.
Assignees
Inventors
Classifications
- C12N9/0044Primary
acting on other nitrogen compounds as donors (1.7) · CPC title
Bacteria; Culture media therefor · CPC title
Nitric-oxide reductase (cytochrome c)(1.7.2.5) · CPC title
Biological processes · CPC title
Enzymatic · CPC title
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- What does patent US12545932B2 cover?
- A recombinant microorganism including a genetic modification that increases activity of nitric oxide reductase in the recombinant microorganism, a composition for reducing a concentration of nitric oxide in a sample, the composition including the recombinant microorganism, and a method of reducing a concentration of nitric oxide in a sample, are disclosed.
- Who is the assignee on this patent?
- Samsung Electronics Co Ltd
- What technology area does this patent fall under?
- Primary CPC classification C12N9/0044. Mapped technology areas include Chemistry & Metallurgy.
- When was this patent published?
- Publication date Tue Feb 10 2026 00:00:00 GMT+0000 (Coordinated Universal Time) (B2). Legal status and post-grant events are not shown on this page.
- What related patents are in patentsdb?
- We list 8 related publications on this page (citations in our corpus or others sharing the same primary CPC).