Ultrafine bubble generating method, ultrafine bubble generating apparatus, and ultrafine bubble-containing liquid
US-11179652-B2 · Nov 23, 2021 · US
Cell culture method, method for producing culture medium, culture medium and culture device
US12545872B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-12545872-B2 |
| Application number | US-202217728574-A |
| Country | US |
| Kind code | B2 |
| Filing date | Apr 25, 2022 |
| Priority date | Oct 31, 2019 |
| Publication date | Feb 10, 2026 |
| Grant date | Feb 10, 2026 |
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Abstract
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A large amount of three-dimensional cells are efficiently produced. To this end, a cell is cultured by using a culture medium containing an ultra-fine bubble generated by heating a heating element to make film boiling on an interface between a liquid W and the heating element.
First claim
Opening claim text (preview).
The invention claimed is: 1 . A method for producing a culture medium used to culture a cell, comprising: an ultra-fine bubble generating step to generate an ultra-fine bubble in a liquid by heating a heating element to make film boiling on an interface between the liquid and the heating element; and a mixing step to generate the culture medium by mixing an ultra-fine bubble-containing liquid containing the ultra-fine bubble generated by the ultra-fine bubble generating step and a liquid containing a predetermined nutrient with each other. 2 . The method according to claim 1 , wherein the liquid used in the ultra-fine bubble generating step is purified water subjected to sterilization processing. 3 . The method according to claim 1 , further comprising: a gas dissolving step to dissolve a predetermined gas into the liquid before the ultra-fine bubble generating step. 4 . The method according to claim 3 , wherein the predetermined gas is a medical gas. 5 . The method according to claim 3 , wherein the predetermined gas contains oxygen. 6 . The method according to claim 1 , further comprising: a vibrating step to apply vibration to the ultra-fine bubble-containing liquid after the ultra-fine bubble generating step. 7 . The method according to claim 6 , wherein the vibration is an ultrasonic wave included within kHz band width to MHz band width. 8 . The method for producing a culture medium according to claim 1 , wherein the ultra-fine bubble contains oxygen. 9 . The method for producing a culture medium according to claim 1 , wherein the cell is a three-dimensional cell that is at least one selected from the group consisting of a spheroid and an organoid. 10 . A method for producing a culture medium used to culture a cell, comprising: an ultra-fine bubble generating step to generate an ultra-fine bubble-containing liquid by generating an ultra-fine bubble in a liquid containing a predetermined nutrient by heating a heating element to make film boiling on an interface between the liquid and the heating element.
Assignees
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Classifications
3D culture · CPC title
Vitamins · CPC title
Amino acids · CPC title
Inorganic components · CPC title
Artificially induced pluripotent stem cells, e.g. iPS · CPC title
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- What does patent US12545872B2 cover?
- A large amount of three-dimensional cells are efficiently produced. To this end, a cell is cultured by using a culture medium containing an ultra-fine bubble generated by heating a heating element to make film boiling on an interface between a liquid W and the heating element.
- Who is the assignee on this patent?
- Canon Kk
- What technology area does this patent fall under?
- Primary CPC classification C12M29/06. Mapped technology areas include Chemistry & Metallurgy.
- When was this patent published?
- Publication date Tue Feb 10 2026 00:00:00 GMT+0000 (Coordinated Universal Time) (B2). Legal status and post-grant events are not shown on this page.
- What related patents are in patentsdb?
- We list 12 related publications on this page (citations in our corpus or others sharing the same primary CPC).