Method to monitor and control the temperature of a sample holder of a laboratory instrument

Patent metadata

Field	Value
Publication number	US-12544760-B2
Application number	US-202218061675-A
Country	US
Kind code	B2
Filing date	Dec 5, 2022
Priority date	Jul 24, 2018
Publication date	Feb 10, 2026
Grant date	Feb 10, 2026

Abstract

A method to monitor and control the temperature of a sample holder of a laboratory instrument during execution of a temperature profile on the sample holder is presented. The laboratory instrument comprises a sample holder with high temperature uniformity and at least three identical temperature sensors. The measured actual temperatures of the sample holder are processed in order to determine if the execution of the temperature profile should be continued or aborted. Furthermore, temperature sensors which measure actual temperatures that do not fulfil certain requirements are excluded from further monitoring and controlling the temperature of a sample holder.

First claim

The invention claimed is:

1 . A laboratory instrument comprising a sample holder with high temperature uniformity, a thermoelectric element which is in thermal contact with the sample holder, and a control device, wherein the sample holder comprises at least three identical temperature sensors, and wherein the control device is adapted to execute the steps of:
a) controlling the thermoelectric element to heat or cool the sample holder to at least one set-point temperature of a temperature profile of the sample holder; b) receiving, via the at least three identical temperature sensors, at least three measured actual temperatures of the sample holder; c) comparing the at least three measured actual temperatures with a predefined maximum permissible temperature deviation range from the at least one set-point temperature; d) calculating at least three temperature differences between each of the at least three measured actual temperatures; e) comparing each of the at least three calculated temperature differences with a predefined maximum permissible temperature difference range; f) determining a further execution step selected from: (i) continuing to execute the temperature profile on the sample holder if at least two of the measured actual temperatures are within the predefined maximum permissible temperature deviation range from the at least one set-point temperature and if at least one of the at least three calculated temperature differences is within the predefined maximum permissible temperature difference range; or (ii) aborting execution of the temperature profile on the sample holder if less than two of the measured actual temperatures are within the predefined maximum permissible temperature deviation range from the at least one set-point temperature and if none of the at least three calculated temperature differences is within the predefined maximum permissible temperature difference range.

2 . The laboratory instrument according to claim 1 , wherein the sample holder comprises a vapor chamber.

3 . The laboratory instrument according to claim 1 , wherein the thermoelectric element comprises an electrodeposited thermoelectric element.

4 . The laboratory instrument according to claim 1 , wherein the control device further comprises a user interface comprising a display, and further wherein the control device is further adapted to display a user notification via the user interface.

5 . The laboratory instrument according to claim 4 , wherein the control device is further adapted to:
exclude the measured actual temperature received from one of the at least three identical temperature sensors when the measured actual temperature of said temperature sensor is outside of the predefined maximum permissible temperature deviation range or results in at least two calculated temperature differences which are not within the predefined maximum permissible temperature difference range; and display a user notification via the user interface regarding a decrease in a number of temperature sensors being used.

6 . The laboratory instrument according to claim 5 , wherein the user notification is displayed on the display and comprises a prompt for preventive maintenance, a warning indicating that exclusion of a further temperature sensor will result in an immediate abort of execution of the temperature profile on the sample holder, or a user interface control element which can be selected by a user in order to continue the execution of the temperature profile on the sample holder or to start a subsequent temperature profile on the sample holder, or a combination thereof.

7 . The laboratory instrument according to claim 1 , wherein the sample holder comprises at least one recess configured to receive a sample vessel comprising a test sample.

Assignees

• Roche Molecular Systems Inc

Inventors

• Ebert Sven
• Federer Paul
• Hermann Pius
• Vollenweider Stefan

Classifications

• G01N25/00

  Investigating or analyzing materials by the use of thermal means (G01N3/00 - G01N23/00 take precedence) · CPC title

• B01L2300/1827

  using resistive heater · CPC title

• B01L2300/1822

  using Peltier elements · CPC title

• B01L2300/0829

  Multi-well plates; Microtitration plates · CPC title

• B01L2300/0627

  Sensor or part of a sensor is integrated · CPC title