Production method for microcapsule, and coating liquid

Patent metadata

Field	Value
Publication number	US-12539277-B2
Application number	US-202117497289-A
Country	US
Kind code	B2
Filing date	Oct 8, 2021
Priority date	Apr 9, 2019
Publication date	Feb 3, 2026
Grant date	Feb 3, 2026

Abstract

There is provided a production method for a microcapsule in which cells are encapsulated with alginic acid and a polycation, the method including dropwise adding a cell suspension containing cells and alginic acid into a calcium ion-containing solution to obtain a liquid droplet in which the cells are encapsulated in alginic acid, and immersing the liquid droplet in a coating liquid containing a polycation, to obtain the microcapsule, where the coating liquid contains a calcium ion or a barium ion at a concentration of 0.1 mM or more and 50.0 mM or less. According to the production method of the present invention, it is possible to produce a microcapsule having a uniform shape and particle size.

First claim

What is claimed is:

1 . A production method for a microcapsule in which cells are encapsulated with alginic acid and a polycation, the method comprising:
dropwise adding a cell suspension containing alginic acid and the cells into a calcium ion-containing solution to obtain a liquid droplet in which the cells are encapsulated with alginic acid; and immersing the liquid droplet in a coating liquid containing a polycation to obtain the microcapsule, wherein the coating liquid contains a calcium ion or a barium ion at a concentration of 0.1 mM or more and 50.0 mM or less.

2 . The production method according to claim 1 , wherein the cell suspension has a viscosity of 50 mPa's or more and 500 mPa's or less.

3 . The production method according to claim 1 , wherein a molecular weight of the polycation is 1 kDa or more and 200 kDa or less.

4 . The production method according to claim 1 , wherein a molecular weight of the polycation is 1 kDa or more and 50 kDa or less.

5 . The production method according to claim 1 , wherein the polycation is poly-L-lysine or poly-L-ornithine.

6 . The production method according to claim 1 , wherein the coating liquid in which the liquid droplet is immersed is subjected to shaking treatment.

7 . The production method according to claim 1 , further comprising immersing the microcapsule in a solution containing alginic acid.

8 . The production method according to claim 7 , wherein the microcapsules after being immersed in the solution containing alginic acid are dispersed in a solution containing a calcium ion or a barium ion at a concentration of 0.1 mM or more and 50.0 mM or less.

9 . The production method according to claim 1 , wherein the cell is a cell that releases a physiologically active substance.

10 . The production method according to claim 9 , wherein the physiologically active substance is insulin.

11 . The production method according to claim 9 , wherein the cell is a cell in pancreatic islet.

12 . The production method according to claim 9 , wherein in the microcapsule, a mesenchymal stem cell or a mesenchymal stem cell group is encapsulated with alginic acid and a polycation together with the cell.

13 . The production method according to claim 1 , wherein the coating liquid contains a calcium ion or a barium ion at a concentration of 0.1 mM or more and 20.0 mM or less.

14 . The production method according to claim 1 , wherein the coating liquid contains a calcium ion or a barium ion at a concentration of 0.2 mM or more and 10.0 mM or less.

15 . The production method according to claim 1 , wherein the coating liquid contains a calcium ion at a concentration of 0.1 mM or more and 50.0 mM or less.

16 . The production method according to claim 1 , wherein the coating liquid contains a calcium ion at a concentration of 0.1 mM or more and 20.0 mM or less.

17 . The production method according to claim 1 , wherein the coating liquid contains a calcium ion at a concentration of 0.2 mM or more and 10.0 mM or less.

Assignees

• Fujifilm Corp

Inventors

• Mochizuki Yusuke
• Takegami Ryuta

Classifications

• A61K47/36

  Polysaccharides; Derivatives thereof, e.g. gums, starch, alginate, dextrin, hyaluronic acid, chitosan, inulin, agar or pectin · CPC title

• A61K38/28

  Insulins · CPC title

• A61K35/28

  Bone marrow; Haematopoietic stem cells; Mesenchymal stem cells of any origin, e.g. adipose-derived stem cells · CPC title

• A61K9/5031

  obtained otherwise than by reactions only involving carbon-to-carbon unsaturated bonds, e.g. polyethylene glycol, poly(lactide-co-glycolide) · CPC title

• A61K9/5089 Primary

  Processes · CPC title