Microorganism producing L-amino acid and method of producing L-amino acid using the same

Patent metadata

Field	Value
Publication number	US-12534747-B2
Application number	US-202017599818-A
Country	US
Kind code	B2
Filing date	Apr 29, 2020
Priority date	May 9, 2019
Publication date	Jan 27, 2026
Grant date	Jan 27, 2026

Abstract

Provided are a microorganism producing an L-amino acid or a precursor thereof, and a method of producing an L-amino acid or a precursor thereof using the microorganism.

First claim

The invention claimed is:

1 . A modified microorganism belonging to the genus Corynebacterium or the genus Escherichia and producing an L-amino acid or a precursor thereof, wherein a protein comprising the amino acid sequence of SEQ ID NO: 1 and being derived from Azotobacter vinelandii is introduced thereinto, and wherein the modified microorganism shows an increased production of the L-amino acid or the precursor thereof compared to a wild-type microorganism belonging to the genus Corynebacterium or the genus Escherichia.

2 . The modified microorganism of claim 1 , wherein the microorganism further has i) weakened phosphoserine phosphatase activity, ii) enhanced 3-phosphoserine aminotransferase activity, or iii) both weakened phosphoserine phosphatase activity and enhanced 3-phosphoserine aminotransferase activity.

3 . The modified microorganism of claim 1 , wherein the microorganism is further modified by enhancement of trp operon, inactivation of tryptophanase (TnaA), inactivation of Mtr membrane protein (Mtr), or any combination thereof.

4 . The modified microorganism of claim 1 , wherein the microorganism further has enhanced his operon.

5 . The modified microorganism of claim 1 , wherein the microorganism is further modified by inactivation of McbR (transcriptional regulator; mcbR), enhancement of methionine synthase (meth), enhancement of sulfite reductase [NADPH] hemoprotein beta-component (cysI), or any combination thereof.

6 . The modified microorganism of claim 1 , wherein the microorganism is Corynebacterium glutamicum or Escherichia coli.

7 . The modified microorganism of claim 1 , wherein the L-amino acid or the precursor thereof is selected from the group consisting of L-serine, L-tryptophan, L-histidine, L-methionine, L-cysteine, O-succinylhomoserine, O-acetylhomoserine, L-homoserine, acetylserine, L-cystathionine, L-homocysteine, and O-phosphoserine.

8 . A method of producing an L-amino acid or a precursor thereof, the method comprising culturing the modified microorganism according to claim 1 in a medium.

9 . The method of claim 8 , further comprising recovering an L-amino acid or a precursor thereof from the cultured microorganism or the culture medium.

10 . The method of claim 8 , wherein the L-amino acid or the precursor thereof is selected from the group consisting of serine, tryptophan, histidine, methionine, L-cysteine, O-succinylhomoserine, O-acetylhomoserine, L-homoserine, acetylserine, L-cystathionine, L-homocysteine, and O-phosphoserine.

11 . A composition for producing an L-amino acid or a precursor thereof, wherein the composition comprises the modified microorganism of claim 1 .

12 . A method of producing an L-amino acid or a precursor thereof using the composition of claim 11 .

Assignees

• Cj Cheiljedang Corp

Inventors

• Yoo Hye Ryun
• Kim So-Yeon
• Park Hye Min
• Lee Sung Gun
• Lee Jin Nam
• Kim Hyun Ah
• Choi Sol
• Huh Lan

Classifications

• C12Y401/99001

  Tryptophanase (4.1.99.1) · CPC title

• C12Y301/03003

  Phosphoserine phosphatase (3.1.3.3) · CPC title

• C12Y206/01052

  Phosphoserine transaminase (2.6.1.52) · CPC title

• C12Y201/01013

  Methionine synthase (2.1.1.13) · CPC title

• C12Y108/01002

  Sulfite reductase (NADPH) (1.8.1.2) · CPC title