Coating film having thinly-spreading and irregularity-conforming properties, and structural base having such film

Patent metadata

Field	Value
Publication number	US-12534637-B2
Application number	US-202418634755-A
Country	US
Kind code	B2
Filing date	Apr 12, 2024
Priority date	Jul 20, 2016
Publication date	Jan 27, 2026
Grant date	Jan 27, 2026

Abstract

The invention provides a structural base that includes a coating film on at least a portion of the surface thereof, the coating film having a difference between the maximum film thickness and the minimum film thickness of not more than 1000 Å. A method of the invention produces such structural bases. The coating film includes a copolymer including a repeating unit containing a group represented by formula (a), and a repeating unit containing a group represented by formula (b):

wherein U a1 , U a2 , U b1 , U b2 , U b3 and An − are as defined herein.

First claim

The invention claimed is:

1 . A method for producing a cell culture vessel for forming cell aggregates, comprising a step of applying a coating agent onto a cell culture vessel including a structural portion composed of at least two flat faces adjacent to one another, the angle θ between each two of the flat faces being 70<θ<110°, in one operation to form a surface coated with a coating film thereby forming a coated surface of the cell culture vessel, the coated surface having a difference between the maximum film thickness and the minimum film thickness of not more than 350 Å and a thickness of 10 to 400 Å,
the coating agent comprising a copolymer including a repeating unit containing a group represented by the following formula (a), and a repeating unit containing a group represented by the following formula (b):

wherein U a1 , U a2 , U b1 , U b2 and U b3 are each independently a hydrogen atom or a C 1-5 linear or branched alkyl group, and An is an anion selected from the group consisting of halide ion, inorganic acid ion, hydroxide ion and isothiocyanate ion.

2 . The production method according to claim 1 , wherein the copolymer includes repeating units represented by the following formulae (a1) and (b1):

wherein
T a and T b are each independently a hydrogen atom or a C 1-5 linear or branched alkyl group;
Q a and Q b are each independently a single bond, an ester bond or an amide bond;
R a and R b are each independently a C 1-10 linear or branched alkylene group which may be substituted with a halogen atom;
U a1 , U a2 , U b1 , U b2 and U b3 are each independently a hydrogen atom or a C 1-5 linear or branched alkyl group;
An − is an anion selected from the group consisting of halide ion, inorganic acid ion, hydroxide ion and isothiocyanate ion; and
m is an integer of 0 to 6.

3 . The production method according to claim 1 , wherein the water contact angle in air of the coated portions of the surface of the cell culture vessel is 0 to 120°, or the bubble contact angle in water of the surface of the structural base is 80 to 180°.

4 . The production method according to claim 1 , wherein the surface that is coated with the coating film is composed of a cycloolefin polymer or polydimethylsiloxane.

5 . A method for forming a coating film on a surface of a cell culture vessel, comprising only steps of applying a coating agent onto the surface of the cell culture vessel and drying it, the coating film having a difference between the maximum film thickness and the minimum film thickness of not more than 350 Å and a film thickness of 10 to 400 Å at all coated portions of the surface of the cell culture vessel, wherein the cell culture vessel includes a structural portion composed of at least two flat faces adjacent to one another, the angle θ between each two of the flat faces being 70<θ<110°,
the coating agent comprising a copolymer including a repeating unit containing a group represented by the following formula (a), and a repeating unit containing a group represented by the following formula (b):

wherein U a1 , U a2 , U b1 , U b2 and U b3 are each independently a hydrogen atom or a C 1-5 linear or branched alkyl group, and An is an anion selected from the group consisting of halide ion, inorganic acid ion, hydroxide ion and isothiocyanate ion.

6 . The method according to claim 5 , wherein the copolymer includes repeating units represented by the following formulae (a1) and (b1):

wherein
T a and T b are each independently a hydrogen atom or a C 1-5 linear or branched alkyl group;
Q a and Q b are each independently a single bond, an ester bond or an amide bond;
R a and R b are each independently a C 1-10 linear or branched alkylene group which may be substituted with a halogen atom;
U a1 , U a2 , U b1 , U b2 and U b3 are each independently a hydrogen atom or a C 1-5 linear or branched alkyl group;
An − is an anion selected from the group consisting of halide ion, inorganic acid ion, hydroxide ion and isothiocyanate ion; and
m is an integer of 0 to 6.

7 . The method according to claim 5 , wherein the water contact angle in air of the coated portions of the surface of the cell culture vessel is 0 to 120° or the bubble contact angle in water of the surface of the structural base is 80 to 180°.

8 . The method according to claim 5 , wherein the surface that is coated with the coating film is composed of a cycloolefin polymer or polydimethylsiloxane.

Assignees

• Nissan Chemical Corp

Inventors

• Hiroi Yoshiomi
• Abe Natsuki
• Nishino Taito

Classifications

• C08G2261/147

  Side-chains with other heteroatoms in the side-chain · CPC title

• C08G2261/143

  Side-chains containing nitrogen · CPC title

• C08G2261/12

  Copolymers · CPC title

• C08G61/04

  only aliphatic carbon atoms · CPC title

• G01N21/7703

  using reagent-clad optical fibres or optical waveguides (using measurement of total internal reflection or attenuated total reflection G01N21/552; optical fibres or waveguides per se G02B) · CPC title