Engineered Nucleic-Acid Targeting Nucleic Acids
US-2018201913-A1 · Jul 19, 2018 · US
Mesophilic argonaute systems and uses thereof
US12534499B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-12534499-B2 |
| Application number | US-202117464635-A |
| Country | US |
| Kind code | B2 |
| Filing date | Sep 1, 2021 |
| Priority date | Mar 6, 2019 |
| Publication date | Jan 27, 2026 |
| Grant date | Jan 27, 2026 |
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- Title
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- Abstract
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Abstract
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Constructs comprising Argonautes and neighboring genes are disclosed for use in gene editing. Disclosed are also compositions and methods utilizing these Argonautes and neighboring genes. Also disclosed are the methods of making and using the Argonautes and neighboring genes in treating various diseases, conditions, and cancer.
First claim
Opening claim text (preview).
What is claimed is: 1 . A system comprising: a mesophilic Argonaute (Ago) polypeptide, or a polynucleic acid encoding the mesophilic Ago polypeptide, or a functional fragment or variant thereof, wherein the Ago polypeptide has an amino acid sequence set forth in any one of SEQ ID NOs 1-10; and an exogenous non-naturally occurring guiding polynucleic acid that binds a target polynucleic acid, wherein upon contacting the target polynucleic acid with the Ago polypeptide and the exogenous non-naturally occurring guiding polynucleic acid at a mesophilic temperature, the Ago polypeptide cleaves the target polynucleic acid. 2 . The system of claim 1 , wherein the Ago polypeptide demonstrates nucleic acid-cleaving activity of the target polynucleic acid at about 37° C. 3 . The system of claim 1 , wherein the guiding polynucleic acid is a guide DNA or a guide RNA. 4 . The system of claim 1 , wherein said system further comprises a nucleic acid unwinding polypeptide or a polynucleic acid encoding the nucleic acid unwinding polypeptide. 5 . The system of claim 4 , wherein said nucleic acid unwinding polypeptide is a helicase, a single strand DNA binding (SSB) protein, or a Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) associated (Cas) protein domain. 6 . The system of claim 5 , wherein said nucleic acid unwinding polypeptide is a single strand DNA binding protein (SSB) polypeptide. 7 . The system of claim 5 , wherein said nucleic acid unwinding polypeptide is a Cas protein domain. 8 . The system of claim 7 , wherein said Cas protein domain is a catalytically dead Cas polypeptide. 9 . The system of claim 1 , wherein said Ago polypeptide is fused either directly or indirectly to a nuclear localization signal (NLS).
Assignees
Inventors
Classifications
involving clustered regularly interspaced short palindromic repeats [CRISPR] · CPC title
containing a DNA binding domain, e.g. Lacl or Tet-repressor · CPC title
DNA helicase (3.6.4.12) · CPC title
DNA or RNA fragments; Modified forms thereof (DNA or RNA not used in recombinant technology, C07H21/00); {Non-coding nucleic acids having a biological activity} · CPC title
Ribonucleases {[RNase]; Deoxyribonucleases [DNase]} · CPC title
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Frequently asked questions
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- What does patent US12534499B2 cover?
- Constructs comprising Argonautes and neighboring genes are disclosed for use in gene editing. Disclosed are also compositions and methods utilizing these Argonautes and neighboring genes. Also disclosed are the methods of making and using the Argonautes and neighboring genes in treating various diseases, conditions, and cancer.
- Who is the assignee on this patent?
- Univ Leland Stanford Junior, Intima Bioscience Inc
- What technology area does this patent fall under?
- Primary CPC classification C07K14/33. Mapped technology areas include Chemistry & Metallurgy.
- When was this patent published?
- Publication date Tue Jan 27 2026 00:00:00 GMT+0000 (Coordinated Universal Time) (B2). Legal status and post-grant events are not shown on this page.
- What related patents are in patentsdb?
- We list 3 related publications on this page (citations in our corpus or others sharing the same primary CPC).