Plants having increased tolerance to herbicides

Patent metadata

Field	Value
Publication number	US-12529066-B2
Application number	US-202318157370-A
Country	US
Kind code	B2
Filing date	Jan 20, 2023
Priority date	Jun 19, 2012
Publication date	Jan 20, 2026
Grant date	Jan 20, 2026

Abstract

The present invention refers to a seed or plant that comprises at least one nucleic acid comprising a nucleotide sequence encoding a wild-type or a mutated protoporphyrinogen oxidase (PPO) which is resistant or tolerant to a benzoxazinone-derivative herbicide. The invention further refers to seeds or plants comprising wild-type or mutated PPO enzymes, and methods of obtaining such seeds or plants.

First claim

The invention claimed is:

1 . A plant comprising a nucleic acid encoding a herbicide tolerant mutant protoporphyrinogen oxidase, (PPO) polypeptide comprising an amino acid substitution at a position corresponding to position Leu397 of SEQ ID NO:2 and an amino acid substitution at a position corresponding to position Phe420 of SEQ ID NO:2, wherein the amino acid corresponding to position Leu397 of SEQ ID NO:2 is substituted with aspartate, glutamate, or glutamine, and wherein the amino acid corresponding to position Phe420 of SEQ ID NO:2 is substituted with valine or methionine,
wherein the plant is selected from the group consisting of Brassica napus, Zea mays, Oryza sativa, Beta vulgaris , and Gossypium hirsutum.

2 . The plant of claim 1 , wherein the amino acid corresponding to position Leu397 of SEQ ID NO:2 is substituted with aspartate and the amino acid corresponding to position Phe420 of SEQ ID NO:2 is substituted with valine.

3 . The plant of claim 1 , wherein the amino acid corresponding to position Leu397 of SEQ ID NO:2 is substituted with glutamate and the amino acid corresponding to position Phe420 of SEQ ID NO:2 is substituted with methionine.

4 . The plant of claim 1 , wherein the amino acid corresponding to position Leu397 of SEQ ID NO:2 is substituted with glutamine and the amino acid corresponding to position Phe420 of SEQ ID NO:2 is substituted with valine.

5 . The plant of claim 1 , wherein the amino acid corresponding to position Leu397 of SEQ ID NO:2 is substituted with aspartate and the amino acid corresponding to position Phe420 of SEQ ID NO:2 is substituted with methionine.

6 . The plant of claim 1 , wherein the amino acid corresponding to position Leu397 of SEQ ID NO:2 is substituted with glutamate and the amino acid corresponding to position Phe420 of SEQ ID NO:2 is substituted with valine.

7 . The plant of claim 1 , wherein the amino acid corresponding to position Leu397 of SEQ ID NO:2 is substituted with glutamine and the amino acid corresponding to position Phe420 of SEQ ID NO:2 is substituted with methionine.

8 . The plant of claim 2 , wherein the plant is non-transgenic.

9 . The plant of claim 3 , wherein the plant is non-transgenic.

10 . The plant of claim 4 , wherein the plant is non-transgenic.

11 . The plant of claim 5 , wherein the plant is non-transgenic.

12 . The plant of claim 6 , wherein the plant is non-transgenic.

13 . The plant of claim 7 , wherein the plant is non-transgenic.

14 . A seed comprising a nucleic acid encoding a herbicide tolerant mutant PPO polypeptide comprising an amino acid substitution at a position corresponding to position Leu397 of SEQ ID NO:2 and an amino acid substitution at a position corresponding to position Phe420 of SEQ ID NO:2, wherein the amino acid corresponding to position Leu397 of SEQ ID NO:2 is substituted with aspartate, glutamate, or glutamine, and wherein the amino acid corresponding to position Phe420 of SEQ ID NO:2 is substituted with valine or methionine,
wherein the seed is from a plant selected from the group consisting of Brassica napus, Zea mays, Oryza sativa, Beta vulgaris , and Gossypium hirsutum.

15 . The seed of claim 14 , wherein the amino acid corresponding to position Leu397 of SEQ ID NO:2 is substituted with aspartate and the amino acid corresponding to position Phe420 of SEQ ID NO:2 is substituted with valine.

16 . The seed of claim 14 , wherein the amino acid corresponding to position Leu397 of SEQ ID NO:2 is substituted with glutamate and the amino acid corresponding to position Phe420 of SEQ ID NO:2 is substituted with methionine.

17 . The seed of claim 14 , wherein the amino acid corresponding to position Leu397 of SEQ ID NO:2 is substituted with glutamine and the amino acid corresponding to position Phe420 of SEQ ID NO:2 is substituted with valine.

18 . The seed of claim 14 , wherein the amino acid corresponding to position Leu397 of SEQ ID NO:2 is substituted with aspartate and the amino acid corresponding to position Phe420 of SEQ ID NO:2 is substituted with methionine.

19 . The seed of claim 14 , wherein the amino acid corresponding to position Leu397 of SEQ ID NO:2 is substituted with glutamate and the amino acid corresponding to position Phe420 of SEQ ID NO:2 is substituted with valine.

20 . The seed of claim 14 , wherein the amino acid corresponding to position Leu397 of SEQ ID NO:2 is substituted with glutamine and the amino acid corresponding to position Phe420 of SEQ ID NO:2 is substituted with methionine.

Assignees

• Basf Agro Bv

Inventors

• Aponte Raphael
• Tresch Stefan
• Witschel Matthias
• Lerchl Jens
• Paulik Jill Marie
• Brommer Chad

Classifications

• C12Y103/03004

  Protoporphyrinogen oxidase (1.3.3.4) · CPC title

• C12N9/0004

  Oxidoreductases (1.) · CPC title

• A01N43/84

  six-membered rings with one nitrogen atom and either one oxygen atom or one sulfur atom in positions 1,4 · CPC title

• G01N2333/90206

  acting on the CH-CH group of donors (1.3) · CPC title

• C12N9/0059

  Catechol oxidase (1.10.3.1), i.e. tyrosinase · CPC title