Substrates having modified surface reactivity and antifouling properties in biological reactions

Patent metadata

Field	Value
Publication number	US-12528083-B2
Application number	US-202117545127-A
Country	US
Kind code	B2
Filing date	Dec 8, 2021
Priority date	May 5, 2017
Publication date	Jan 20, 2026
Grant date	Jan 20, 2026

Abstract

Integrated devices including substrates with sample wells having modified surfaces are provided. An integrated device includes a substrate with a sample well having a metal oxide surface and a silica surface, a coating layer on the metal oxide surface formed by an amphipathic reagent attached to a polymeric compound, an antifouling overlay on the coating layer formed by a block copolymer, and a functionalizing agent bound to the silica surface.

First claim

The invention claimed is:

1 . An integrated device comprising:
a substrate comprising a sample well having a metal oxide surface and a silica surface; a coating layer on the metal oxide surface formed by an amphipathic reagent attached to a polymeric compound; an antifouling overlay on the coating layer formed by a block copolymer; and a functionalizing agent bound to the silica surface.

2 . The integrated device of claim 1 , wherein the substrate comprises an array of sample wells, each sample well having the metal oxide surface and the silica surface.

3 . The integrated device of claim 1 , wherein the amphipathic reagent comprises a hydrophilic head group bound to the metal oxide surface.

4 . The integrated device of claim 1 , wherein the polymeric compound comprises poly(ethylene glycol), poly(ethylene oxide), poly(propylene glycol), poly(butylene glycol), or a combination thereof.

5 . The integrated device of claim 1 , wherein the block copolymer comprises an A-B-A type block copolymer comprising an A block and a B block, wherein the A-B-A type block copolymer is bound to the coating layer through the B block.

6 . The integrated device of claim 1 , wherein the metal oxide surface is aluminum oxide, titanium oxide, zirconium oxide, iron oxide, tin oxide, or tantalum oxide.

Assignees

• Quantum Si Inc

Inventors

• Rothberg Jonathan M
• Lackey Jeremy
• Chen Guojun

Classifications

• B01L2200/12

  Specific details about manufacturing devices · CPC title

• B01L2300/0819

  Microarrays; Biochips · CPC title

• B01L2300/16

  Surface properties and coatings · CPC title

• B01L3/5085 Primary

  for multiple samples, e.g. microtitration plates · CPC title

• C09D171/02

  Polyalkylene oxides · CPC title