Salt-tolerant heterotrophic nitrification aerobic denitrification phosphorus removal bacterial strain and application thereof

Patent metadata

Field	Value
Publication number	US-12522801-B2
Application number	US-202318535656-A
Country	US
Kind code	B2
Filing date	Dec 11, 2023
Priority date	Dec 13, 2022
Publication date	Jan 13, 2026
Grant date	Jan 13, 2026

Abstract

The application relates to the field of microbiology and presents a salt-tolerant heterotrophic nitrification aerobic denitrifying and phosphorus removal bacterial strain and its application. The strain is Pseudomonas mendocina A4, deposited on Nov. 4, 2022, at the Guangdong Microbial Culture Collection Center, located at Building 59, 5th Floor, No. 100 Xianlie Middle Road, Yuexiu District, Guangzhou, Guangdong Province, with the deposit number GDMCC No: 62944. The Pseudomonas mendocina A4 strain provided by the present application possesses both heterotrophic nitrification and aerobic denitrification functions and can be applied in the field of wastewater treatment. It has a strong tolerance to high concentrations of organic carbon while utilizing various organic carbon sources in wastewater and exhibits excellent organic carbon removal capabilities in water. This strain is particularly suitable for treating nitrogen-containing wastewater with a high C/N ratio.

First claim

What is claimed is:

1 . An application of a salt-tolerant heterotrophic nitrification aerobic denitrification and phosphorus removal bacterial strain, wherein Pseudomonas mendocina A4 is used in a field of wastewater treatment by applying the bacterial strain in wastewater, where the wastewater is nitrogen and phosphorus-containing wastewater in saltwater aquaculture.

2 . The application of the salt-tolerant heterotrophic nitrification aerobic denitrification and phosphorus removal bacterial strain as claimed in claim 1 , wherein a carbon source of the wastewater is at least one of sodium citrate, sodium succinate, sucrose, and glucose.

3 . The application of the salt-tolerant heterotrophic nitrification aerobic denitrification and phosphorus removal bacterial strain as claimed in claim 2 , wherein the carbon source of the wastewater is sodium succinate.

4 . The application of the salt-tolerant heterotrophic nitrification aerobic denitrification and phosphorus removal bacterial strain as claimed in claim 1 , wherein Pseudomonas mendocina A4 is used in the treatment of nitrogen and phosphorus-containing wastewater, with a C/N ratio of 0 to 15.

5 . The application of the salt-tolerant heterotrophic nitrification aerobic denitrification and phosphorus removal bacterial strain as claimed in claim 1 , wherein Pseudomonas mendocina A4 is used in the treatment of nitrogen and phosphorus-containing wastewater, with a C/N ratio of 10.

6 . The application of the salt-tolerant heterotrophic nitrification aerobic denitrification and phosphorus removal bacterial strain as claimed in claim 1 , wherein Pseudomonas mendocina A4 is used in the treatment of nitrogen and phosphorus-containing wastewater, with a P/N ratio of 0.1 to 1.

7 . The application of the salt-tolerant heterotrophic nitrification aerobic denitrification and phosphorus removal bacterial strain as claimed in claim 1 , wherein Pseudomonas mendocina A4 is used in the treatment of nitrogen and phosphorus-containing wastewater, with a pH value of 5 to 9.

8 . The application of the salt-tolerant heterotrophic nitrification aerobic denitrification and phosphorus removal bacterial strain as claimed in claim 1 , wherein Pseudomonas mendocina A4 is used in the treatment of nitrogen and phosphorus-containing wastewater, at a temperature of 25° C. to 40° C.

9 . The application of the salt-tolerant heterotrophic nitrification aerobic denitrification and phosphorus removal bacterial strain as claimed in claim 1 , wherein a salinity of the wastewater is 0.5% to 15%.

Assignees

• Univ Guangzhou

Inventors

• Shu Hu
• Ma Yonghao
• Huang Wen
• Han Chong

Classifications

• Y02W10/10

  Biological treatment of water, waste water, or sewage · CPC title

• C12N1/20

  Bacteria; Culture media therefor · CPC title

• C02F3/341

  Consortia of bacteria · CPC title

• C02F2305/06

  Nutrients for stimulating the growth of microorganisms · CPC title

• C02F2101/166

  Nitrites · CPC title