Culturing method and culturing device

Patent metadata

Field	Value
Publication number	US-12522796-B2
Application number	US-202217681943-A
Country	US
Kind code	B2
Filing date	Feb 28, 2022
Priority date	Mar 31, 2021
Publication date	Jan 13, 2026
Grant date	Jan 13, 2026

Abstract

In a culturing method, microalgae are cultured in a culturing solution while a gas containing carbon dioxide is supplied to the culturing solution. In an ion concentration acquisition step, an acquired value of a concentration of hydrogen carbonate ions in the culturing solution is obtained. In an ion concentration adjustment step, in the case that the acquired value does not reside within a set concentration range that is set beforehand, at least one of a temperature or a pH of the culturing solution is adjusted, whereby the concentration of hydrogen carbonate ions in the culturing solution is adjusted to reside within the set concentration range.

First claim

What is claimed is:

1 . A culturing method for culturing microalgae in a culturing solution while a gas containing carbon dioxide is supplied to the culturing solution, the culturing method comprising:
an ion concentration acquisition step of obtaining an acquired value of a concentration of hydrogen carbonate ions in the culturing solution; and an ion concentration adjustment step of, in a case that the acquired value does not reside within a set concentration range that is set beforehand, performing at least one of adjusting of a molar fraction of the hydrogen carbonate ions in the culturing solution by adjusting a pH of the culturing solution or adjusting of an amount of carbon dioxide dissolved in the culturing solution by adjusting a temperature of the culturing solution without adjusting a concentration of carbon dioxide in the gas, and thereby adjusting the concentration of hydrogen carbonate ions in the culturing solution to reside within the set concentration range.

2 . The culturing method according to claim 1 , wherein in the ion concentration adjustment step, in a case of adjusting the pH of the culturing solution, hydrochloric acid is added to the culturing solution.

3 . The culturing method according to claim 1 , wherein in the ion concentration adjustment step, the temperature of the culturing solution is adjusted so as to become a set temperature that is set beforehand, and based on a relationship between the pH and a molar fraction of the hydrogen carbonate ions when the culturing solution is at the set temperature, a target value of the pH at which the concentration of hydrogen carbonate ions in the culturing solution resides within the set concentration range is calculated, and the pH of the culturing solution is adjusted to the target value.

4 . The culturing method according to claim 3 , further comprising:
a determination step of determining, prior to the ion concentration adjustment step, whether or not the acquired value resides within the set concentration range; in the determination step, in a case it is determined that the acquired value does not reside within the set concentration range, a further determination is made as to whether the acquired value exceeds or falls below the set concentration range; and in the ion concentration adjustment step: in a case it is determined in the determination step that the acquired value exceeds the set concentration range, the set temperature is set to an upper limit value of a set temperature range that is set beforehand; and in a case it is determined in the determination step that the acquired value falls below the set concentration range, the set temperature is set to a lower limit value of the set temperature range.

5 . The culturing method according to claim 1 , wherein in the ion concentration acquisition step, the acquired value is obtained by a calculation based on a carbon dioxide concentration measurement value of the gas supplied to the culturing solution, a pH measurement value of the culturing solution, and a temperature measurement value of the culturing solution.

6 . A culturing device configured to culture microalgae in a culturing solution while a gas containing carbon dioxide is supplied to the culturing solution, the culturing device comprising:
a culturing tank configured to contain the culturing solution and the microalgae, and to which the gas from a gas supplying unit is supplied; an ion concentration acquisition unit configured to obtain an acquired value of a concentration of hydrogen carbonate ions in the culturing solution inside the culturing tank; a temperature adjustment unit configured to adjust a temperature of the culturing solution; a pH adjustment unit configured to adjust a pH of the culturing solution; and an ion concentration adjustment unit configured to compare the acquired value and a set concentration range that is set beforehand, and to, when the acquired value does not reside within the set concentration range, perform at least one of adjusting of a molar fraction of the hydrogen carbonate ions in the culturing solution by adjusting a pH of the culturing solution or adjusting of an amount of carbon dioxide dissolved in the culturing solution by adjusting a temperature of the culturing solution without adjusting a concentration of carbon dioxide in the gas, and thereby perform an adjustment of the concentration of hydrogen carbonate ions in the culturing solution inside the culturing tank.

7 . The culturing device according to claim 6 , wherein the pH adjustment unit adjusts the pH of the culturing solution by adding hydrochloric acid to the culturing solution.

8 . The culturing device according to claim 6 , further comprising:
a carbon dioxide sensor configured to measure a carbon dioxide concentration of the gas supplied from the gas supplying unit; an in-tank temperature sensor configured to measure the temperature of the culturing solution inside the culturing tank; and an in-tank pH sensor configured to measure the pH of the culturing solution inside the culturing tank; wherein the ion concentration acquisition unit obtains the acquired value by a calculation based on a carbon dioxide concentration measurement value of the carbon dioxide sensor, a temperature measurement value of the in-tank temperature sensor, and a pH measurement value of the in-tank pH sensor.

9 . The culturing device according to claim 6 , wherein:
a guide member extending along a vertical direction is provided in an interior of the culturing tank; and by the gas being supplied upwardly, the guide member draws in the culturing solution from below the guide member, and discharges the culturing solution from above the guide member, thereby generating a culturing solution flow inside the culturing tank.

Assignees

• Honda Motor Co Ltd

Inventors

• Goto Minoru
• Shiobara Nozomi
• Kinoshita Shohei
• Takano Fumitomo
• Doi Mizuho
• Shiozaki Satoshi
• Machida Kenji

Classifications

• C12M29/18

  External loop; Means for reintroduction of fermented biomass or liquid percolate (loop type reactors for chemical or physical processes B01J19/2435) · CPC title

• C12M41/34

  of gas · CPC title

• C12M29/06

  Nozzles; Sprayers; Spargers; Diffusers (per se B01F23/231, B01J19/26) · CPC title

• C12M41/26

  of pH · CPC title

• C12M41/32

  of substances in solution · CPC title