Cell culture device

Patent metadata

Field	Value
Publication number	US-12522792-B2
Application number	US-202218089570-A
Country	US
Kind code	B2
Filing date	Dec 28, 2022
Priority date	Dec 28, 2022
Publication date	Jan 13, 2026
Grant date	Jan 13, 2026

Abstract

A cell culture device configured for activating, transducing, isolating and/or expanding cells. The cell culture device includes a mixing tank and a rotary control platform. The mixing tank includes a first support, a hollow tank and a cover. The hollow tank is disposed on the first support and has a first opening, a second opening and an accommodating space. The cover covers the second opening and has a first hole, an inlet end and an outlet end. The rotary control platform includes a base, a second support, a rotating gear, an inclination control member and a micro injector. The second support is disposed on the base. The rotating gear is fixed on the first support. The inclination control member has a first part, a second part and a third part. The first part is connected to the rotating gear, and the second part is connected to the first part and the third part. The micro injector extends to the accommodating space through the first hole of the cover. The micro injector has a top end and a bottom end, the top end is fixed on the third part, and the bottom end is disposed to a bottom of the accommodating space. The micro injector is inclined towards the bottom of the accommodating space.

First claim

What is claimed is:

1 . A cell culture device for activating, transducing, isolating, and/or expanding cells, comprising:
a mixing tank, wherein the mixing tank comprises:
a first support;
a hollow tank, disposed on the first support and has a first opening, a second opening, and an accommodating space; and
a cover, covering the second opening and has a first hole, an inlet end, and an outlet end; and
a rotary control platform, wherein the rotary control platform comprises:
a base;
a second support, disposed on the base;
a rotating gear, fixed on the second support;
an inclination control member, having a first part, a second part, and a third part, wherein the first part is connected to the rotating gear, and the second part is connected to the first part and the third part; and
a micro injector, extending to the accommodating space through the first hole of the cover, wherein the micro injector has a top end and a bottom end, the top end is fixed on the third part, and the bottom end is disposed on a bottom of the accommodation space, wherein the micro injector is inclined towards the bottom of the accommodating space through an adjustable angle between the first part and the second part; and

wherein the first support has a support member and a gas-permeable film, the support member has a plurality of micropores arranged in an array, the gas-permeable film is disposed between the hollow tank and the support member, and the gas-permeable film covers the first opening of the hollow tank.

2 . The cell culture device according to claim 1 , wherein the gas-permeable film has pores with diameter of 0.1 micron to 0.3 micron, and a thickness of the gas-permeable film is 50 microns to 500 microns.

3 . The cell culture device according to claim 1 , wherein a number of the plurality of micropores is 40 to 60 and the plurality of micropores have pores with diameter of 1 mm to 2 mm.

4 . The cell culture device according to claim 1 , wherein the mixing tank further comprises: a first pipeline, connected to the inlet end and extending to a top of the accommodating space; a second pipeline, connected to the outlet end and extending to the bottom of the accommodation space; a silicone sheet, disposed between the cover and the hollow tank, covering the second opening and has a second hole; a first sealing member, disposed at a joint between the hollow tank and the first support; and a second sealing member, disposed at a joint between the cover and the hollow tank, wherein the micro injector extends to the accommodating space through the second hole of the silicone sheet.

5 . The cell culture device according to claim 1 , wherein a rotation angle of the rotating gear is 0° to 360°.

6 . The cell culture device according to claim 1 , wherein the rotary control platform further comprises: a motor, disposed on the second support and driving the rotating gear; and a pump, having a third pipeline, wherein the third pipeline is connected to the top of the micro injector through the third part of the inclination control member.

7 . The cell culture device according to claim 1 , wherein an inclination angle between an extending direction of the micro injector and a horizontal surface of the first support is 30° to 85°.

8 . The cell culture device according to claim 1 , wherein a first width of the top end of the micro injector is larger than a second width of the bottom end of the micro injector.

9 . The cell culture device according to claim 8 , wherein a ratio of the first width to the second width is 5:1 to 8:1.

Assignees

• Ind Tech Res Inst

Inventors

• Wu Li-An
• Chen Ting-Hsuan
• Lee Chih-Hung
• Chou Nien-Jen
• Wen Kuo-Hsing
• Chiang Wen-Ting

Classifications

• C12M33/04

  by injection or suction, e.g. using pipettes, syringes, needles (pipettes in general B01L3/02) · CPC title

• C12M1/265

  Pipettes; Syringes; Suction devices · CPC title

• C12M35/00 Primary

  Means for application of stress for stimulating the growth of microorganisms or the generation of fermentation or metabolic products; Means for electroporation or cell fusion (machines for extracting juice from animal or plant tissue by electroplasmolysis A23N1/006, processes employing electric or wave energy B01J19/08; treatment of microorganisms or enzymes with electrical or wave energy C12N13/00; methods for cell fusion C12N15/02; introduction of foreign genetic material C12N15/87) · CPC title

• C12M27/16

  Vibrating; Shaking; Tilting · CPC title

• C12M23/24

  Gas permeable parts · CPC title