Container and test kit

Patent metadata

Field	Value
Publication number	US-12521718-B2
Application number	US-202217670721-A
Country	US
Kind code	B2
Filing date	Feb 14, 2022
Priority date	Sep 27, 2019
Publication date	Jan 13, 2026
Grant date	Jan 13, 2026

Abstract

In a container including two accommodation parts each accommodating liquid, mixing of the liquids in a case of moving magnetic particles in the liquid accommodated in one accommodation part to the other accommodation part is suppressed. Provided is a container includes a first accommodation part which accommodates a first liquid containing magnetic particles, a second accommodation part which accommodates separated magnetic particles separated from the first liquid, and a second liquid, and a flow passage which allows the first accommodation part and the second accommodation part to communicate with each other, and through which the separated magnetic particles pass, in which the flow passage has a first staircase part including two or more steps from an inner bottom surface of the first accommodation part on a first accommodation part side.

First claim

What is claimed is:

1 . A container comprising:
a first accommodation part which accommodates a first liquid containing magnetic particles, the first accommodation part being a chamber containing the first liquid; a second accommodation part which accommodates separated magnetic particles separated from the first liquid, and a second liquid different from the first liquid, the second accommodation part being a chamber containing the second liquid, wherein the second liquid does not include a liquid extracted or separated from the first liquid; and a flow passage which allows the first accommodation part and the second accommodation part to communicate with each other, and through which the separated magnetic particles pass, wherein the flow passage has a first staircase part including two or more steps from an inner bottom surface of the first accommodation part on a first accommodation part side, the first staircase part being a barrier for suppressing entry of the first liquid into the flow passage, wherein a height of the flow passage is smaller than a height of the first accommodation part and a height of the second accommodation part, wherein a width of the flow passage is narrower than a width of the first accommodation part and a width of the second accommodation part, and wherein a water contact angle of at least a part of an inner surface of the first accommodation part is smaller than a water contact angle of at least a part of an inner surface of the flow passage.

2 . The container according to claim 1 ,
wherein the flow passage allows the first accommodation part and the second accommodation part to communicate with each other at upper end positions thereof.

3 . The container according to claim 1 ,
wherein a height of a first step of the first staircase part with respect to the inner bottom surface of the first accommodation part is 25% to 80% of a height of the first accommodation part.

4 . The container according to claim 2 ,
wherein a height of a first step of the first staircase part with respect to the inner bottom surface of the first accommodation part is 25% to 80% of a height of the first accommodation part.

5 . The container according to claim 1 ,
wherein a height of a second step of the first staircase part with respect to the inner bottom surface of the first accommodation part is 50% to 96% of a height of the first accommodation part.

6 . The container according to claim 2 ,
wherein a height of a second step of the first staircase part with respect to the inner bottom surface of the first accommodation part is 50% to 96% of a height of the first accommodation part.

7 . The container according to claim 3 ,
wherein a height of a second step of the first staircase part with respect to the inner bottom surface of the first accommodation part is 50% to 96% of a height of the first accommodation part.

8 . The container according to claim 4 ,
wherein a height of a second step of the first staircase part with respect to the inner bottom surface of the first accommodation part is 50% to 96% of a height of the first accommodation part.

9 . The container according to claim 1 ,
wherein the water contact angle of at least a part of the inner surface of the flow passage is 90° to 180°.

10 . The container according to claim 1 ,
wherein the water contact angle of at least a part of the inner surface of the flow passage is 120° to 180°.

11 . The container according to claim 1 ,
wherein the water contact angle of at least a part of the inner surface of the first accommodation part is 0° to 60°.

12 . The container according to claim 1 ,
wherein the water contact angle of at least a part of the inner surface of the first accommodation part is 0° to 30°.

13 . The container according to claim 1 ,
wherein an angle of at least one step of the first staircase part is an acute angle in a cross section parallel to an extending direction of the flow passage and parallel to a direction perpendicular to the flow passage.

14 . The container according to claim 1 ,
wherein the flow passage has a second staircase part including two or more steps from an inner bottom surface of the second accommodation part on a second accommodation part side.

15 . The container according to claim 1 ,
wherein the magnetic particles are magnetic particles that adsorb nucleic acid.

16 . The container according to claim 15 , further comprising:
a chromatographic carrier for performing a test of the nucleic acid; and a carrier accommodation part for accommodating the chromatographic carrier.

17 . A test kit comprising:
the container according to claim 1 ; and magnetic particles.

18 . The test kit according to claim 17 , further comprising:
a nucleic acid extraction liquid, a cleaning liquid, and at least one solution of an amplification liquid or a detection pretreatment liquid.

Assignees

• Fujifilm Corp

Inventors

• Hama Takeshi
• Komori Noboru
• Inoue Yuki
• Ouchi Aya

Classifications

• G01N33/521

  Single-layer analytical elements · CPC title

• C12Q1/6806

  Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay (C12Q1/6804 takes precedence) · CPC title

• B01L2400/0644

  rotary valves · CPC title

• B01L2400/043

  magnetic forces · CPC title

• B01L2300/069

  Absorbents; Gels to retain a fluid · CPC title