Monocot leaf explant preparation
US-2025134021-A1 · May 1, 2025 · US
US12520772B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-12520772-B2 |
| Application number | US-202318453908-A |
| Country | US |
| Kind code | B2 |
| Filing date | Aug 22, 2023 |
| Priority date | Aug 22, 2022 |
| Publication date | Jan 13, 2026 |
| Grant date | Jan 13, 2026 |
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Disclosed herein is a system and methods for leaf detection and extraction. The extracted leaf may be used for leaf water potential analysis. In some embodiments, the method comprises identifying the leaf from a point cloud based on an image, determining a pose of the leaf based on the point cloud, and cutting and storing the leaf based on the pose of the leaf.
Opening claim text (preview).
What is claimed is: 1 . A computer-implemented method for collecting one or more leaves, comprising: receiving, from a camera, an image comprising a leaf; generating, based on the image, a point cloud; identifying a portion of the point cloud associated with the leaf; determining, based on the portion of the point cloud, a pose of the leaf, wherein: the pose comprises a position and an orientation of the leaf, an end-effector is aligned to the leaf based on the pose of the leaf, and a pneumatic subsystem is enabled; enclosing the leaf in a chamber of the end-effector, wherein, after the aligning the end-effector to the leaf, the chamber of the end-effector is moved toward the leaf to enclose the leaf; cutting, via a blade of the end-effector, a stem of the leaf; and storing the leaf in the chamber of the end-effector. 2 . The method of claim 1 , wherein: the end-effector is coupled to an arm; and the arm aligns the end-effector to the leaf and moves the end-effector toward the leaf. 3 . The method of claim 1 , wherein: the end-effector comprises the camera; and the camera takes images of the leaves as it moves toward the leaf. 4 . The method of claim 1 , wherein: the end-effector actuates a slider for occluding the chamber, wherein: the slider is coupled to the blade and a piston; and the piston actuates the slider and the blade. 5 . The method of claim 1 wherein: the end-effector actuates a gate for occluding the chamber, wherein: the gate is coupled to the blade and two four-bar linkages; and the two four-bar linkages actuate the gate and the blade. 6 . The method of claim 1 , wherein: the point cloud comprises a second portion, the second portion comprising points at distances greater than a threshold distance from the camera, and the method further comprises: identifying the second portion; and disregarding the second portion for the identifying the portion of the point cloud associated with the leaf. 7 . The method of claim 1 , wherein the identifying the portion of the point cloud associated with the leaf comprises: downsampling the portion of the point cloud; and clustering the portion of the point cloud. 8 . The method of claim 1 , wherein the determining the pose of the leaf comprises: bounding a clustered portion of the point cloud; and determining a center, dimensions, and orientation of the bounded cluster. 9 . The method of claim 1 , wherein the image comprises a second leaf, the method further comprising: identifying a second portion of the point cloud associated with the second leaf; determining, based on the second portion of the point cloud, a second pose of the second leaf, wherein: the second pose comprises a position and an orientation of the second leaf, and the end-effector is aligned to the second leaf based on the second pose of the second leaf; enclosing the second leaf in the chamber of the end-effector, wherein, after the aligning the end-effector to the second leaf, the chamber of the end-effector is moved toward the second leaf to enclose the second leaf; cutting, via the blade of the end-effector, a stem of the second leaf; and storing the second leaf in the chamber of the end-effector. 10 . The method of claim 9 , wherein the end-effector is aligned to the second leaf, suction is enabled, the end-effector is moved toward the second leaf, the stem of the second leaf is cut, and the second leaf is stored in accordance with a determination that the leaf is damaged. 11 . The method of claim 1 , wherein the pneumatic subsystem actuates suction within the end-effector using a solenoid, an air compressor, and an air tank. 12 . The method of claim 1 , further comprising: receiving, from the camera, a second image different from the image; generating, based on the second image, a second point cloud; and identifying a portion of the second point cloud, wherein the portion of the point cloud is associated with a second leaf. 13 . The method of claim 1 , wherein the determining the pose of the leaf further comprises identifying the stem of the leaf, a tip of the leaf, and a centroid of the leaf. 14 . An end-effector for collecting one or more leaves, comprising: a camera configured to take images of the leaves to send to the processor; a blade configured to cut the leaves; a chamber to encapsulate the leaves for cutting and storage; and one or more processors configured to cause the end-effector to perform the method of claim 1 . 15 . The end-effector of claim 14 , wherein a weight of the end-effector is less than 1.3 kg. 16 . The end-effector of claim 14 , wherein the chamber has a width of 110 mm, a height of 45 mm, and a depth of 185 mm. 17 . The end-effector of claim 14 , wherein a force for cutting the stem is 2.9-20 N, or operates at a pressure no greater than 120 psi. 18 . The end-effector of claim 14 , wherein the camera comprises a depth camera configured to provide images of the leaf to transmit to the processor for leaf detection. 19 . The end-effector of claim 14 , wherein: the end-effector further comprises a slider for occluding the chamber, the slider is coupled to the blade and a piston, and the piston is configured to actuate the slider and the blade. 20 . The end-effector of claim 14 , wherein: the end-effector further comprises a gate for occluding the chamber, the gate is coupled to the blade and two four-bar linkages, and the two four-bar linkages are configured to actuate the gate and the blade. 21 . A system for collecting one or more leaves, comprising: an end-effector comprising: a camera configured to take images of the one or more leaves to send to one or more processors; a blade configured to cut the one or more leaves; a chamber to encapsulate the one or more leaves for cutting and storage; and wherein the one or more processors are configured to cause the end-effector to perform the method of claim 1 ; a pneumatic system configured to use suction to align the one or more leaves; an arm coupled to the end-effector and configured to move the end-effector with six degrees of freedom; and a base coupled to the arm and comprising wheels. 22 . The system of claim 21 , wherein the pneumatic system comprises an air compressor, an air tank, and a solenoid.
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