Compact multi-color flow cytometer

Patent metadata

Field	Value
Publication number	US-12517034-B2
Application number	US-202016920618-A
Country	US
Kind code	B2
Filing date	Jul 3, 2020
Priority date	Apr 26, 2016
Publication date	Jan 6, 2026
Grant date	Jan 6, 2026

Abstract

A system, an apparatus, and a method are provided for a modular flow cytometer with a compact size. In one embodiment, the modular flow cytometry system includes the following: a laser system for emitting laser beams; a flow cell assembly positioned to receive the laser beams at an interrogation region of a fluidics stream where fluoresced cells scatter the laser beams into fluorescent light; a fiber assembly positioned to collect the fluorescent light; and a grating system including a dispersive element and a receiver assembly, wherein the dispersive element is positioned to receive the fluorescent light from the fiber assembly and to direct spectrally dispersed light toward the receiver assembly.

First claim

What is claimed is:

1 . A compact modular flow cytometry system, comprising:
a modular enclosure with a compact footprint configured to rest onto a bench top, the modular enclosure having at least one lower shelf below a top of the modular enclosure; an optical plate assembly coupled to the top of the modular enclosure; a laser system with a plurality of excitation lasers coupled to the optical plate to emit a plurality of laser beams; a flow cell assembly coupled to the optical plate assembly, the flow cell assembly positioned to receive the plurality of laser beams at an interrogation region of a fluidics stream where fluoresced cells scatter the plurality of laser beams into fluorescent light; at least one fiber assembly having at least one fiber head at one end coupled to the optical plate assembly, the at least one fiber head having an input end of at least one collection fiber positioned to collect the fluorescent light; and at least one light-receiving system coupled to the at least one lower shelf of the modular enclosure, the at least one light-receiving system including a dispersive element and a light receiver assembly, wherein the dispersive element is positioned to receive the fluorescent light from an output end of the at least one collection fiber and to direct spectrally dispersed light toward the light receiver assembly, wherein the at least one fiber assembly has flexibility that enables the at least one light-receiving system to be located on the at least one lower shelf of the modular enclosure.

2 . The compact modular flow cytometry system of claim 1 , wherein:
the at least one light-receiving system is mounted to the optical plate assembly.

3 . The compact modular flow cytometry system of claim 1 , wherein:
the at least one fiber assembly directs the fluorescent light from the optical plate coupled to the top of the modular enclosure towards the at least one light-receiving system mounted into the lower shelf of the modular enclosure.

4 . The compact modular flow cytometry system of claim 1 , wherein:
the at least one light-receiving system further includes a collimating lens positioned between the output end of the at least one collection fiber and the dispersive element.

5 . The compact modular flow cytometry system of claim 1 , wherein:
the dispersive element of the at least one light-receiving system is a diffraction grating to disperse the fluorescent light.

6 . The compact modular flow cytometry system of claim 5 , wherein the receiver assembly comprises:
a photodiode detector array positioned to receive the spectrally dispersed light, wherein the photodiode detector array includes a plurality of channels; and trans-impedance amplifier circuits configured to convert input current from the photodiode detector array into an output voltage signal for each channel of the photodiode detector array.

7 . The compact modular flow cytometry system of claim 6 , wherein the receiver assembly further comprises:
analog-to-digital converters coupled to trans-impedance amplifier circuits, wherein each trans-impedance amplifier circuit is coupled to a different analog-to-digital converter.

8 . The compact modular flow cytometry system of claim 1 , wherein:
the dispersive element of the at least one light-receiving system is a linear graded long pass filter to disperse the fluorescent light.

9 . The compact modular flow cytometry system of claim 1 , wherein the receiver assembly comprises:
a photodiode detector array positioned to receive the spectrally dispersed light, wherein the photodiode detector array includes a plurality of channels; and trans-impedance amplifier circuits configured to convert input current from the photodiode detector array into an output voltage signal for each channel of the photodiode detector array; and analog-to-digital converters coupled to trans-impedance amplifier circuits, wherein each trans-impedance amplifier circuit is coupled to a different analog-to-digital converter.

10 . The compact modular flow cytometry system of claim 1 , further comprising:
a modular fluidics assembly mounted to a front side of the modular enclosure near the flow cell assembly, the modular fluidics assembly including
a pair of bulkhead connectors for liquid input/output (I/O) ports coupled to tank lines,
one bulkhead connector for input sheath fluid, and
another bulkhead connector for output waste fluid in the front of the modular fluidics assembly.

11 . The compact modular flow cytometry system of claim 1 , further comprising:
an electrical panel assembly mounted to the modular enclosure including a general purpose power supply isolated from at least one analog power supply supplying power to a photodiode detector array and trans-impedance amplifiers in the receiver assembly of the at least one light-receiving system.

12 . The compact modular flow cytometry system of claim 1 , further comprising:
a sample injection tube assembly mounted within the modular enclosure, the sample injection tube assembly providing z-axis sample loading functionality; and a loader assembly including a plate loader with x-θ stage motor.

Assignees

• Cytek Biosciences Inc

Inventors

• Vrane David
• Yan Ming
• Chase Eric
• Jiang Wenbin

Classifications

• G01N15/01

  specially adapted for biological cells, e.g. blood cells (investigating sedimentation of particle suspensions in blood G01N15/05) · CPC title

• G01N2201/068

  Optics, miscellaneous · CPC title

• G01N2021/6421

  Measuring at two or more wavelengths · CPC title

• G01N2015/1006

  for cytology · CPC title

• G01N2201/08

  Optical fibres; light guides · CPC title