System and method for sperm sorting

Patent metadata

Field	Value
Publication number	US-12517030-B2
Application number	US-202418882174-A
Country	US
Kind code	B2
Filing date	Sep 11, 2024
Priority date	Nov 20, 2013
Publication date	Jan 6, 2026
Grant date	Jan 6, 2026

Abstract

A system and method for sorting sperm is provided. The system includes a housing and a microfluidic system supported by the housing. The system also includes an inlet providing access to the microfluidic system to deliver sperm to the microfluidic system and an outlet providing access to the microfluidic system to harvest sorted sperm from the microfluidic system. The microfluidic system provides a flow path for sperm from the inlet to the outlet and includes at least one channel extending from the inlet to the outlet to allow sperm delivered to the microfluidic system through the inlet to progress along the flow path toward the outlet. The microfluidic system also includes a filter including a first plurality of micropores arranged in the flow path between the inlet and the outlet to cause sperm traveling along the flow path to move against through the filter and gravity to reach the outlet.

First claim

The invention claimed is:

1 . A sperm sorting assembly comprising:
a first component defining a cavity; a second component configured to be disposed below and coupled to the first component, the second component defining a reservoir configured to hold a sample of sperm; and a filter configured to be horizontally disposed between the first component and the second component, the filter comprising a plurality of pores sized to permit motile sperm of the sample of sperm to pass vertically therethrough from the reservoir of the second component to the cavity of the first component when the second component is disposed below and coupled to the first component and the sample of sperm is held in the reservoir of the second component; wherein the first component defines a recess in fluid communication with the cavity, the recess extends from a top outer surface of the first component, and wherein the recess is sized and configured to facilitate harvesting motile sperm of the sample of s perm out of the recess; wherein a first component wall defining the cavity of the first component is configured to mate with a second component wall defining the reservoir of the second component when the second component is disposed below and coupled to the first component.

2 . The sperm sorting assembly of claim 1 , wherein the cavity is circular.

3 . The sperm sorting assembly of claim 1 , wherein the first component entirely defines the recess.

4 . The sperm sorting assembly of claim 1 , wherein the recess is physically isolated from the second component.

5 . The sperm sorting assembly of claim 1 , wherein the recess comprises a channel that extends radially outwardly from an opening defined by the first component, the opening being adjacent the filter.

6 . The sperm sorting assembly of claim 5 , wherein the channel is spaced from the filter.

7 . The sperm sorting assembly of claim 6 , wherein the channel is vertically spaced from the filter when the second component is disposed below and coupled to the first component and the filter is horizontally disposed between the first component and the second component.

8 . The sperm sorting assembly of claim 1 , wherein the filter is attached to the first component.

9 . The sperm sorting assembly of claim 8 , wherein the filter is attached to a bottom surface of the first component.

10 . The sperm sorting assembly of claim 1 , wherein the filter is disposed across an opening defined by the first component.

11 . The sperm sorting assembly of claim 10 , wherein the opening is circular.

12 . The sperm sorting assembly of claim 10 , wherein the filter and the opening are arranged to allow motile sperm of the sample of sperm to travel from the reservoir defined by the second component through the filter, through the opening, and into the cavity defined by the first component.

13 . The sperm sorting assembly of claim 1 , wherein the recess is above the filter when the second component is disposed below and coupled to the first component and the filter is horizontally disposed between the first component and the second component.

14 . The sperm sorting assembly of claim 1 , wherein the recess is spaced from the filter, and wherein a portion of the first component separates the recess from the filter.

15 . The sperm sorting assembly of claim 14 , wherein the recess is configured such that when harvesting the motile sperm using a harvesting tool from the recess, the portion of the first component blocks the filter from being contacted by the harvesting tool.

16 . The sperm sorting assembly of claim 15 , wherein the recess has an open top end that is sized to receive a tip of the harvesting tool, and wherein the open top end is above the recess when the second component is disposed below and coupled to the first component and the filter is horizontally disposed between the first component and the second component.

17 . The sperm sorting assembly of claim 1 , wherein the recess is exposed to an exterior of the first component, and
wherein the recess is configured to receive an end of a syringe from the exterior of the first component to facilitate harvesting motile sperm.

18 . The sperm sorting assembly of claim 1 , wherein the recess comprises a substantially horizontal channel defined by the first component and an opening in the top outer surface of the first component that is in fluid communication with the substantially horizontal channel, the opening being sized to allow an end of a harvesting tool to be inserted into the substantially horizontal channel from an exterior of the first component to facilitate harvesting motile sperm from the substantially horizontal channel.

19 . The sperm sorting assembly of claim 1 , wherein the first component wall and the second component wall are complementary shaped.

20 . The sperm sorting assembly of claim 1 , wherein the first component wall comprises a bottom outer surface facing in an opposite direction of the top outer surface of the first component and the second component wall comprises a top outer surface, and wherein when the second component is disposed below and coupled to the first component, the bottom outer surface of the first component wall mates with the top outer surface of the second component wall.

21 . A sperm sorting assembly comprising:
a first component comprising a top outer surface, the first component defining a recess extending from the top outer surface; a second component configured to be disposed below and coupled to the first component, the second component defining a cavity configured to receive a sample of sperm; a filter configured to be horizontally disposed between the first component and the second component; wherein when the sperm sorting assembly is assembled with the second component being disposed below and coupled to the first component and the filter being horizontally disposed between the first component and the second component, the sperm sorting assembly comprises:
an upper chamber in fluid communication with the recess, the upper chamber at least partially defined by the filter and the first component; and
a lower chamber in fluid communication with the cavity and the upper chamber, the lower chamber at least partially defined by the filter and the second component;
wherein the filter defines a plurality of pores sized to permit motile sperm of the sample of sperm to pass vertically therethrough from the lower chamber to the upper chamber when the sample of sperm is in the lower chamber; and
wherein the recess is sized and configured to facilitate harvesting at least some of the motile sperm of the sample of sperm out of the recess without contacting the filter;
wherein a first component wall of the first component at least partially defining the upper chamber is configured to mate with a second component wall defining the cavity of the second component when the second component is disposed below and coupled to the first component.

22 . The sperm sorting assembly of claim 21 , wherein the recess is physically isolated from the second component when the sperm sorting assembly is assembled.

23 . The sperm sorting assembly of claim 21 , wherein the recess comprises a channel extends radially outwardly from an opening defined by the first component, the opening being adjacent to the filter.

24 . The sperm sorting assembly of claim 23 , wherein the channel is vertically spaced from the fil

Assignees

• Brigham & Womens Hospital Inc

Inventors

• Demirci Utkan
• Asghar Waseem

Classifications

• G01N2015/1006

  for cytology · CPC title

• G01N2015/1028

  Sorting particles · CPC title

• G01N15/01

  specially adapted for biological cells, e.g. blood cells (investigating sedimentation of particle suspensions in blood G01N15/05) · CPC title

• G01N2001/4088

  filtration · CPC title

• C12N5/061

  Sperm cells, spermatogonia · CPC title