Method for predicting the clinical response of oncolytic parvovirus H1 (H-1PV) treatment in a patient suspected of suffering from cancer by measuring the expression levels of laminins and/or galectins as biomarkers in a patient's sample

Patent metadata

Field	Value
Publication number	US-12516381-B2
Application number	US-202117307236-A
Country	US
Kind code	B2
Filing date	May 4, 2021
Priority date	Nov 22, 2018
Publication date	Jan 6, 2026
Grant date	Jan 6, 2026

Abstract

The present invention concerns the use of laminins and/or galectins as biomarker(s) for predicting the outcome of the treatment with oncolytic parvovirus H1 (H-1 PV) in a cancer patient.

First claim

What is claimed is:

1 . A method of detecting an elevated level of a biomarker as compared with a predetermined reference level of the biomarker for determining whether a human patient suspected to suffer from cancer is a candidate for H-1PV therapy for said cancer, and treatment of said cancer thereof, comprising;
(a) obtaining a biological sample from the patient, the biological sample comprising a tissue biopsy, blood sample, serum sample, or plasma sample; and (b) subjecting the patient's biological sample to at least one assay to measure the presence of the biomarker; and (c) detecting the elevated level of the biomarker when the measured level of the biomarker exceeds the predetermined reference level of the biomarker by at least threefold, whereby detecting the elevated level of the biomarker as compared with that of the predetermined reference level of the biomarker indicates that the patient will benefit from a H-1PV therapy; and (d) administering H-1PV therapy to the patient having the elevated level of the biomarker; wherein the biomarker comprises laminin and/or galactin; and wherein the predetermined reference level of the biomarker is determined as a value between the 25th and 75th percentile of the overall distribution of biomarker measurements from samples obtained from healthy individuals or from a population of patients within the same disease entity.

2 . The method according to claim 1 , wherein the laminin is LAMB1 and/or LAMC1.

3 . The method according to claim 1 , wherein the galectin is LGALS1.

4 . The method according to claim 1 , wherein the cancer is a solid tumour.

5 . The method of claim 4 , wherein the solid tumor is a brain cancer, lung cancer, breast cancer, skin cancer, colon cancer, pancreatic cancer, or hepatocellular carcinoma.

6 . The method according to claim 5 , wherein the brain cancer is glioblastoma; wherein the lung cancer is non-small cell lung carcinoma; wherein the pancreatic cancer is pancreatic ductal adenocarcinoma; wherein the breast cancer is invasive ductal carcinoma; wherein the skin cancer is melanoma; and wherein the colon cancer is colon adenocarcinoma.

7 . The method of claim 6 , wherein the non-small lung carcinoma is lung adenocarcinoma or lung squamous cell carcinoma.

8 . The method according to claim 1 , wherein the cancer is selected from kidney renal papillary cell carcinoma (KIRP), brain lower grade glioma (LGG), stomach adenocarcinoma (STAD), bladder urothelial carcinoma (BLCA), cervical squamous cell carcinoma, endocervical adenocarcinoma (CESC), and rectum adenocarcinoma (READ).

9 . The method according to claim 1 , wherein the cancer from brain lower grade glioma (LGG), kidney renal clear cell carcinoma (KIRC), and acute myeloid leukemia (LAML).

Assignees

• Deutsches Krebs Forschungszentrum Stiftung Des Oeffentlichen Rechts
• Deutsches Krebsforschungszentrum Stiftung Des Oeffentlichen Rechts

Inventors

• Marchini Antonio
• Kulkarni Amit
• Grewenig Annabel
• Rommelaere Jean
• Marttila Tiina
• Ferreira Tiago

Classifications

• C12Q2600/158

  Expression markers · CPC title

• C12Q2600/106

  Pharmacogenomics, i.e. genetic variability in individual responses to drugs and drug metabolism · CPC title

• C12N2750/14032

  Use of virus as therapeutic agent, other than vaccine, e.g. as cytolytic agent · CPC title

• A61K35/768

  Oncolytic viruses not provided for in groups A61K35/761 - A61K35/766 · CPC title

• G01N2800/52

  Predicting or monitoring the response to treatment, e.g. for selection of therapy based on assay results in personalised medicine; Prognosis · CPC title