Microorganism having high ability to degrade oil at low temperature

Patent metadata

Field	Value
Publication number	US-12515972-B2
Application number	US-202017640296-A
Country	US
Kind code	B2
Filing date	Sep 7, 2020
Priority date	Sep 6, 2019
Publication date	Jan 6, 2026
Grant date	Jan 6, 2026

Abstract

In an aspect, the present disclosure provides a novel microorganism having an ester degrading ability. In an aspect, the present disclosure provides a combination of novel microorganisms having an oil degrading ability. In an embodiment, the microorganism of the present invention includes a yeast belonging to the genus Yarrowia . In an embodiment, the microorganism of the present invention includes Yarrowia lipolytica . In an embodiment, the present disclosure provides a combination of a Burkholderia bacterium with a Yarrowia yeast. In an embodiment, provided is an oil degrading agent that comprises the microorganism of the present disclosure.

First claim

The invention claimed is:

1 . A method of treating oil and fat or fatty acid, comprising contacting oil and fat or fatty acid with a combination of a Yarrowia yeast which produces lipase and a Burkholderia bacterium which secretes lipase.

2 . The method of claim 1 , wherein the Yarrowia yeast is Yarrowia lipolytica.

3 . The method of claim 1 , wherein the Burkholderia bacterium is a bacterium in the genus Burkholderia.

4 . The method of claim 1 , wherein the Burkholderia bacterium is one of Burkholderia arboris, Burkholderia ambifaria , and Burkholderia cepacia complex.

5 . The method of claim 1 , wherein the combination of a Burkholderia bacterium and a Yarrowia yeast has a higher ability to decompose oil and fat or fatty acid than an ability to decompose oil and fat or fatty acid calculated from a value of an ability to decompose oil and fat or fatty acid of single culture of each of the Burkholderia bacterium and the Yarrowia yeast.

6 . The method of claim 1 , wherein a cell count of the Burkholderia bacterium: a cell count of the Yarrowia yeast is 1:20 to 20:1.

7 . The method of claim 1 , wherein at least one of the Burkholderia bacterium and the Yarrowia yeast has an ability to decompose fatty acid at 15° C.

8 . The method of claim 1 , wherein the combination is comprised in an oil entity decomposing agent.

9 . The method of claim 1 , further comprising contacting the oil and fat or fatty acid with an oil entity treating component.

10 . A method of decomposing and removing oil entity, comprising causing a combination to act on a subject of treatment, wherein the combination is a combination of a Yarrowia yeast which produces lipase and a Burkholderia bacterium which produces lipase, wherein the Yarrowia yeast is capable of decomposing oil and fat.

11 . The method of claim 1 , wherein a cell count of the Burkholderia bacterium: a cell count of the Yarrowia yeast is 1:9 to 9:1.

Assignees

• National Univ Corporation Tokai National Higher Education And Research System

Inventors

• Hori Katsutoshi

Classifications

• C12N1/20

  Bacteria; Culture media therefor · CPC title

• C02F2103/32

  from the food or foodstuff industry, e.g. brewery waste waters · CPC title

• C02F2101/32

  Hydrocarbons, e.g. oil · CPC title

• C02F3/343 Primary

  for digestion of grease, fat, oil · CPC title

• C12R2001/645

  Fungi {; Processes using fungi} · CPC title