Plant exosomes used for treatment of diseases originating from protein aggregates formed in cells

Patent metadata

Field	Value
Publication number	US-12514890-B2
Application number	US-202017631967-A
Country	US
Kind code	B2
Filing date	Jul 30, 2020
Priority date	Aug 1, 2019
Publication date	Jan 6, 2026
Grant date	Jan 6, 2026

Abstract

Plant exosomes can be used to reduce the protein aggregates formed in the cells and the damage they cause to the cells. Plant exosomes are of plant origin and not transgenic, and thus, they are reliable as an alternative to the drugs used in the treatment of neurodegenerative diseases, particularly ALS. Moreover, compared to the conventional applications, plant exosomes can provide a product with high efficacy and no side effects which can be produced in high amounts and at affordable prices due to the fact that it is plant-derived.

First claim

What is claimed is:

1 . A method of reducing protein aggregates formed in cells, comprising the step of administering plant exosomes to the cells in vitro; wherein the protein aggregates formed in the cells are associated with a neurodegenerative disease; and wherein the neurodegenerative disease is at least one selected from the group consisting of: Alzheimer's disease, Parkinson's disease, Huntington's disease, Amyotrophic Lateral Sclerosis (ALS) disease, and prion disease.

2 . The method according to claim 1 , wherein the neurodegenerative disease is the Amyotrophic Lateral Sclerosis (ALS) disease.

3 . The method according to claim 1 , wherein the plant exosomes are administered at an effective dose within an exosome value range of 10 μg-1 mg.

4 . The method according to claim 1 , wherein the plant exosomes are obtained by an isolation from at least one plant selected from the group consisting of a pomegranate plant, a pineapple plant, and a celery plant.

5 . The method according to claim 1 , wherein the plant exosomes are obtained upon an isolation from at least one plant material selected from the group consisting of an entire plant, a fruit, a leaf, a seed, a root, a plant culture medium, a stem cell, a waste material, a shell, and a phloem.

6 . The method according to claim 5 , wherein the plant exosomes are obtained upon the isolation from the plant material by at least one isolation method selected from the group consisting of two-phase separation, graduated centrifuge, ultrafiltration, chromatographic methods, polymer-based isolation, and isolation by microbeads.

7 . The method according to claim 4 , wherein the plant exosomes are obtained upon the isolation from at least one plant material selected from the group consisting of an entire plant, a fruit, a leaf, a seed, a root, a plant culture medium, a stem cell, a waste material, a shell, and a phloem.

Assignees

• Univ Yeditepe

Inventors

• Sahin Fikrettin
• Kirbas Oguz Kaan
• Bozkurt Batuhan Turhan
• Tasli Pakize Neslihan

Classifications

• A61P25/28

  for treating neurodegenerative disorders of the central nervous system, e.g. nootropic agents, cognition enhancers, drugs for treating Alzheimer's disease or other forms of dementia · CPC title

• A61K36/88

  Liliopsida (monocotyledons) · CPC title

• A61K36/23

  Apiaceae or Umbelliferae (Carrot family), e.g. dill, chervil, coriander or cumin · CPC title

• A61K36/185 Primary

  Magnoliopsida (dicotyledons) · CPC title