Genetically modified bacillus subtilis strain and use as a live delivery and production system
US-2024390433-A1 · Nov 28, 2024 · US
US12509398B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-12509398-B2 |
| Application number | US-202217825182-A |
| Country | US |
| Kind code | B2 |
| Filing date | May 26, 2022 |
| Priority date | May 26, 2022 |
| Publication date | Dec 30, 2025 |
| Grant date | Dec 30, 2025 |
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The present disclosure provides a Paenibacillus mucilaginosus LT1906 and use thereof, and a microorganism-activated circulating fluidized bed (CFB) fly ash-based cementitious material and a preparation method and use thereof, belonging to the technical field of cementitious materials. The Paenibacillus mucilaginosus LT1906 has a deposit number of CGMCC No. 21337. The strain is capable of dissolving silicate minerals and aluminosilicate minerals in the CFB fly ash to increase dissolution of active SiO 2 . The strain is used for activating the CFB fly ash-based cementitious material, to ameliorate a large water demand and a low early strength of the cementitious material, thereby improving comprehensive utilization of the CFB fly ash in the field of building materials.
Opening claim text (preview).
What is claimed is: 1 . A microorganism-activated circulating fluidized bed (CFB) fly ash-based cementitious material, comprising the following raw materials: CFB fly ash, a bacterial solution of the Paenibacillus mucilaginosus LT1906 with a deposit number of China General Microbial Culture Collection Center (CGMCC) No. 21337, and a vinegar residue leachate. 2 . The microorganism-activated CFB fly ash-based cementitious material according to claim 1 , wherein the CFB fly ash, the bacterial solution of the Paenibacillus mucilaginosus LT1906 and the vinegar residue leachate have a mass ratio of 100:9-27:18-36. 3 . The microorganism-activated CFB fly ash-based cementitious material according to claim 1 , wherein the CFB fly ash is a solid waste generated by burning coal in a CFB boiler, comprises the following components in mass percentages: SiO 2 ≥35%, f-CaO≤4%, and SO 3 ≤3%, and has a specific surface area of 330 m 2 /kg to 400 m 2 /kg and a 80 μm sieve residue of 15% to 20%. 4 . The microorganism-activated CFB fly ash-based cementitious material according to claim 1 , wherein the bacterial solution of the Paenibacillus mucilaginosus LT1906 has a cell concentration of 10 6 cells/mL to 10 7 cells/mL and a viscosity of 2 mPa·s to 15 mPa·s. 5 . The microorganism-activated CFB fly ash-based cementitious material according to claim 1 , wherein a preparation method of the vinegar residue leachate comprises the following steps: mixing water with a fresh vinegar residue in a mass ratio of 800-1000:1, allowing to stand at 4° C. to 10° C. for 24 h to 36 h, and removing a solid vinegar residue by filtration to obtain the vinegar residue leachate.
Bacterial isolates · CPC title
Living organisms, e.g. microorganisms, or enzymes · CPC title
Bacillus · CPC title
Carboxylic acids; Salts, anhydrides or esters thereof · CPC title
containing hydraulic cements other than calcium sulfates · CPC title
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