Solid reagent containment unit, in particular for a transportable microfluidic device for sample preparation and molecule analysis

Patent metadata

Field	Value
Publication number	US-12508591-B2
Application number	US-202217981299-A
Country	US
Kind code	B2
Filing date	Nov 4, 2022
Priority date	Dec 28, 2017
Publication date	Dec 30, 2025
Grant date	Dec 30, 2025

Abstract

A solid reagent containment unit is formed by a support; a frame body fixed to the support and delimiting internally, together with the support, an analysis volume; a reagent-adhesion structure within the analysis volume; and at least one reagent cavity, which extends within the reagent-adhesion structure. The reagent-adhesion structure is of an adhesion material embossable at temperatures lower by 6-8° C. than its own melting point and has a melting point such as not to interfere with the analysis. The reagent cavity forms a retention wall, laterally surrounding the reagent cavity, and houses dried reagents. The adhesion material is chosen among wax, such as paraffin, a polymer, such as polycaprolactone, a solid fat, such as cocoa butter, and a gel, such as hydrogel or organogel.

First claim

The invention claimed is:

1 . A method, comprising:
introducing a sample into an extraction chamber of a portable microfluidic device by introducing the sample into a sample inlet opening of the portable microfluidic device and passing the sample through an inlet fluidic recess in fluid communication with the sample inlet opening and the extraction chamber, the extraction chamber being defined by a first wall of the portable microfluidic device, a second wall of the portable microfluidic device, and a body of the portable microfluidic device that is between the first wall and the second wall; extracting molecules from the sample in the extraction chamber to obtain an analyzable sample; transferring the analyzable sample to an analysis chamber of the portable microfluidic device delimited by the body and the first wall; exposing the analyzable sample to a solid reagent, the solid reagent being in a reagent cavity of a reagent unit and the solid reagent being on a surface of an integrated circuit chip of the reagent unit, the reagent cavity being delimited by a sidewall of an embossed holding material of the reagent unit and a surface of the integrated circuit chip of the reagent unit; and analyzing the analyzable sample exposed to the solid reagent within the reagent cavity.

2 . The method of claim 1 , wherein extracting molecules from the sample in the extraction chamber to obtain the analyzable sample includes:
introducing a preparation reagent into the extraction chamber in which the sample and a plurality of magnetic beads are present, introducing the preparation reagent into the extraction chamber includes: activating a light emitting diode (LED) to open a one-shot valve along the inlet fluidic recess of the portable microfluidic device; opening a first valve hole by deforming a first shutter; and generating a suction pressure with a pump; rotating and translating an anchor of an anchor actuator in the extraction chamber to stir and mix the preparation reagent, the sample, and the plurality of magnetic beads in the extraction chamber extracting the molecules from the sample and adhering the plurality of magnetic beads to the molecules; removing the preparation reagents from the extraction chamber while leaving the plurality of magnetic beads and the molecules within the extraction chamber by opening a second valve hole through deforming a second shutter and activating a blocking actuator attracting the plurality of magnetic beads and holding the plurality of magnetic beads to which the molecules are adhered within the extraction chamber; flushing the molecules by introducing at least one flushing liquid into the extraction chamber; and forming the analyzable sample by introducing an elution liquid separating the molecules from the plurality of magnetic beads.

3 . The method of claim 2 , wherein transferring the analyzable sample to the analysis chamber of the portable microfluidic device delimited by the body and the first wall further includes opening a third valve hole along a fluidic pathway that extends from the extraction chamber to the analysis chamber by deforming a third shutter and generating the suction pressure with the pump.

4 . The method of claim 2 , wherein removing the preparation reagent from the extraction chamber includes discharging the preparation reagent from the portable microfluidic device through a fluidic outlet of the portable microfluidic device.

5 . The method of claim 2 , wherein removing the preparation reagent includes introducing the preparation reagent to a waste chamber within the portable microfluidic device delimited by the first wall, the second wall, and the body.

6 . The method of claim 2 , further comprising heating the solid reagent with a heater of the integrated circuit aligned with the reagent cavity in which the solid reagent is present.

7 . A method, comprising:
introducing a sample into an extraction chamber of a portable microfluidic device; dissolving a tablet containing a plurality of magnetic beads, the tablet being dissolved by the sample and being present within the extraction chamber, wherein dissolving the tablet releases the plurality of magnetic beads within the extraction chamber, the extraction chamber being defined by a first wall of the portable microfluidic device, a second wall of the portable microfluidic device, and a body of the portable microfluidic device that is between the first wall and the second wall; extracting molecules from the sample in the extraction chamber to obtain an analyzable sample including: introducing preparation reagents into the extraction chamber in which the sample is present; rotating and translating an anchor of an anchor actuator within the extraction chamber to stir and mix the sample with the preparation reagents to extract the molecules from the sample and to adhere the molecules to the magnetic beads; magnetically attracting the magnetic beads towards a blocking actuator by activating the blocking actuator; and discarding spent preparation reagents spent from extracting the molecules from the sample; transferring the analyzable sample to an analysis chamber of the portable microfluidic device delimited by the body and the first wall; exposing the analyzable sample to a solid reagent, the solid reagent being in a reagent cavity of a reagent unit and the solid reagent being on a surface of an integrated circuit chip of the reagent unit, the reagent cavity being delimited by a sidewall of an embossed holding material of the reagent unit and a surface of the integrated circuit chip of the reagent unit; and analyzing the analyzable sample exposed to the solid reagent within the reagent cavity.

8 . The method of claim 7 , wherein extracting the molecules from the extraction chamber to obtain the analyzable sample further includes:
introducing a preparation reagent into the extraction chamber in which the sample and magnetic beads are present, introducing the preparation reagent into the extraction chamber includes: activating a light emitting diode (LED) to open a one-shot valve along an inlet fluidic recess of the portable microfluidic device; opening a first valve hole by deforming a first shutter; and generating a suction pressure with a pump; flushing the molecules by introducing one or more flushing liquids into the extraction chamber; and forming the analyzable sample by introducing an elution liquid to separate the molecules from the magnetic beads.

9 . The method of claim 8 , wherein flushing the molecules by introducing the one or more flushing liquids into the extraction chamber further includes introducing a plurality of flushing liquids into the extraction chamber.

10 . The method of claim 9 , wherein introducing the plurality of flushing liquids into the extraction chamber further includes introducing in sequence each respective liquid of the plurality of flushing liquids into the extraction chamber.

11 . The method of claim 10 , wherein the plurality of flushing liquids are stored within a plurality of reagent chambers within the portable microfluidic device, and the plurality of reagent chambers are delimited by the first wall, the second wall, and the body.

12 . The method of claim 10 , wherein the molecules are nucleic acids present within the sample.

13 . A method, comprising:
introducing a sample into an extraction chamber of a portable microfluidic device by introducing the sample into a sample inlet opening of the portable microfluidic device and passing the sample through an inlet fluidic recess in fluid communication with the sample inlet opening and the extraction chamber, the extractio

Assignees

• St Microelectronics Srl

Inventors

• Cereda Marco
• Raia Lillo
• Bramanti Alessandro Paolo

Classifications

• C12N15/1003

  Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor · CPC title

• B01L2400/0421

  electrophoretic flow · CPC title

• B01L2200/0631

  Purification arrangements, e.g. solid phase extraction [SPE] · CPC title

• B01L3/502753

  characterised by bulk separation arrangements on lab-on-a-chip devices, e.g. for filtration or centrifugation · CPC title

• B01L2200/16

  Reagents, handling or storing thereof · CPC title