Integrated synthesis and analysis systems and methods of use thereof

Patent metadata

Field	Value
Publication number	US-12505900-B2
Application number	US-202117923709-A
Country	US
Kind code	B2
Filing date	Mar 18, 2021
Priority date	May 20, 2020
Publication date	Dec 23, 2025
Grant date	Dec 23, 2025

Abstract

The invention generally relates to integrated synthesis and analysis systems and methods of use thereof.

First claim

What is claimed is:

1 . A method for forming a chemical product and conducting one or more biological assays on the chemical product, the method comprising:
forming a chemical product in a desorption electrospray ionization (DESI) droplet product from directing a DESI spray from a DESI source onto a substrate to desorb and ionize chemical molecules and form the chemical product, wherein the forming step is conducted for each spot from a surface of an array of spots; analyzing the chemical product in a mass spectrometer; collecting the chemical product; incubating the chemical product with one or more biological molecules; and analyzing results of the incubation of the chemical product with the one or more biological molecules; wherein all steps of the method are performed by an integrated synthesis and analysis system that comprises a fluid handling apparatus comprising one or more robotic instruments configured to handle and move the chemical product through each step of the method and a control module comprising software to control and coordinate operation of each of the method steps.

2 . The method of claim 1 , wherein the control module further comprises software that generates a heat map of the chemical product from each spot to illustrate successful formation of the chemical product for each spot.

3 . The method of claim 2 , wherein the software analyzes the heat map and determines which chemical products should be rescanned for structural information.

4 . The method of claim 1 , wherein the forming step further utilizes:
a two-position DESI stage wherein a first position is configured for analysis of the chemical product and is proximate an inlet of the mass spectrometer and a second position located away from the inlet of the mass spectrometer; and a switch controlled by the control module to switch between the first and second position.

5 . The method of claim 1 , wherein the incubating is for one or more biological assays selected from the group consisting of: cytotoxicity, enzyme reactivation, antibiotic activity, binding affinity, enzyme inhibition, antiviral activity, agonism/antagonism, and blood/brain barrier penetration.

6 . The method of claim 1 , wherein the method further comprises a second characterization step in a second characterization position that is accessible by the fluid handling apparatus of the control module and that can be operable coupled to the control module.

7 . The method of claim 6 , wherein the second characterization comprises an analysis by Raman spectroscopy.

8 . The method of claim 1 , wherein analyzing results of the incubation comprises use of a multiplexed inductive nano-electrospray and micro-electrophoresis apparatus to achieve rapid in situ sample clean-up and ionization using small volume samples.

9 . The method of claim 8 , wherein the control module controls operation of the multiplexed inductive nano-electrospray and micro-electrophoresis apparatus.

10 . The method of claim 1 , wherein the chemical product is comprises a non-addictive pain medication.

Assignees

• Purdue Research Foundation

Inventors

• Cooks Robert Graham
• Morato Nicolás M

Classifications

• H01J49/165

  Electrospray ionisation · CPC title

• G01N33/6851

  Methods of protein analysis involving laser desorption ionisation mass spectrometry · CPC title

• G16C20/50

  Molecular design, e.g. of drugs · CPC title

• G16C20/20

  Identification of molecular entities, parts thereof or of chemical compositions · CPC title

• B01J2219/00317

  Microwell devices, i.e. having large numbers of wells · CPC title