Compositions, apparatuses and methods for making and using bioscaffolds

Patent metadata

Field	Value
Publication number	US-12503686-B2
Application number	US-202117558543-A
Country	US
Kind code	B2
Filing date	Dec 21, 2021
Priority date	Dec 21, 2020
Publication date	Dec 23, 2025
Grant date	Dec 23, 2025

Abstract

The present disclosure relates to compositions, apparatus and methods for generating one or more scaffolds, including: mixing a hydrogel material and/or an extracellular matrix (ECM) protein in an aqueous solvent to generate an aqueous process solution; and cryoelectrospinning the aqueous process solution onto a plurality of conductive probes extending from a conductive surface of a collector plate disposed within a process chamber under conditions sufficient to generate one or more scaffolds configured to mimic a preselected soft tissue decellularized extracellular matrix. Scaffold compositions are also provided having preselected or tuned characteristics.

First claim

What is claimed is:

1 . A scaffold comprising: a cryoelectrospun alginate, electrospun collagen, and elastin material that mimics decellularized ECM, wherein the alginate and elastin are cross-linked.

2 . The scaffold of claim 1 , wherein the scaffold is characterized as lyophilized scaffold, and wherein the lyophilized scaffold comprises 99.9 percent weight to 0.1 percent weight alginate, and 0.1 percent weight to 99.9 percent weight elastin, wherein the percent weight is the percent weight of the total lyophilized scaffold.

3 . The scaffold of claim 2 , wherein the lyophilized scaffold is further characterized as rehydrated.

4 . The scaffold of claim 1 , wherein the scaffold further comprises one or more biodegradable or biocompatible polymers.

5 . The scaffold of claim 1 , wherein the scaffold further comprises natural polymer, collagen, cryoelectrospun collagen, gelatin, silk fibroin, hyaluronic acid, chitosan, agarose, synthetic polymer, poly(alpha esters), poly(lactate acid), poly(glycolic acid), polyorthoesters, polyanhydrides and their copolymers, or combinations thereof.

6 . The scaffold of claim 1 , wherein the scaffold further comprises one or more ECM proteins such as collagen, elastin, laminin, and combinations thereof.

7 . The scaffold of claim 1 , wherein the scaffold is characterized as a cryoelectrospun scaffold that mimics the decellularized ECM of one or more soft tissue, salivary tissue, mammary tissue, heart tissue, pancreatic tissue, or the like.

8 . The scaffold of claim 1 , wherein the scaffold is characterized as a scaffold that mimics a decellularized ECM of one or more soft tissue organs such as salivary tissue, lung tissue, or liver tissue, and the like.

9 . The scaffold of claim 1 , wherein, when hydrated, one or more fibers of the scaffold have a similar organization to native ECM of a preselected organ or preselected tissue.

10 . The scaffold of claim 1 , wherein the scaffold is capable of supporting growth and/or differentiation of one or more cells to become fibrotic cells, mimicking fibrotic tissues, or to serve as fibrosis model.

Assignees

• Univ New York State Res Found

Inventors

• Sharfstein Susan
• Xie Yubing
• Ramesh Pujhitha
• Castracane James
• Larsen Melinda

Classifications

• C12N2535/00

  Supports or coatings for cell culture characterised by topography · CPC title

• B82Y5/00

  Nanobiotechnology or nanomedicine, e.g. protein engineering or drug delivery · CPC title

• C12N2533/90

  Substrates of biological origin, e.g. extracellular matrix, decellularised tissue · CPC title

• C12N2533/74

  Alginate · CPC title

• C12N2533/72

  Chitin, chitosan · CPC title