Anti-phenacetin monoclonal antibody hybridoma cell strain ad and its preparation method and application

Patent metadata

Field	Value
Publication number	US-12503519-B2
Application number	US-202117779989-A
Country	US
Kind code	B2
Filing date	Nov 9, 2021
Priority date	Mar 1, 2021
Publication date	Dec 23, 2025
Grant date	Dec 23, 2025

Abstract

The invention discloses an anti-phenacetin monoclonal antibody hybridoma cell strain AD, a preparation method and application thereof, and relates to the technical field of food safety immunodetection. The monoclonal antibody hybridoma cell strain is named monoclonal cell strain AD and the number CGMCC19681. The Phe-BA obtained by the hydrolysis of the reaction product of the phenacetin metabolite acetaminophen and ethyl 4-bromobutyrate is used as the hapten, and the hapten is coupled with the carrier protein to prepare the immunogen Phe-BA-BSA. After the mice were immunized with the immunogen Phe-BA-BSA, they were fused with myeloma cells by PEG method, screened by indirect competitive enzyme-linked immunosorbent assay and subcloned five times to obtain hybridoma cell lines. The monoclonal antibody secreted by the cell line can be made into a phenacetin detection kit, which has good affinity and detection sensitivity for phenacetin, and can be used for immunodetection of phenacetin residues in food.

First claim

What is claimed is:

1 . A hybridoma cell strain AD with anti-phenacetin monoclonal antibody, deposited at the China General Microbiological Culture Collection Center with Accession Number CGMCC No. 19681.

2 . An anti-phenacetin monoclonal antibody secreted by the monoclonal hybridoma cell strain AD of claim 1 .

3 . A method for analyzing and detecting phenacetin residues in food comprising:
adding the an anti-phenacetin monoclonal antibody of claim 2 to a subject to form a detection subject, and using the detection subject for analyzing and detecting phenacetin residues in a food sample, wherein the detection subject is a reagent, a detection plate, or a kit.

Assignees

• Univ Jiangnan

Inventors

• Xu Chuanlai
• Yao Jingjing
• Kuang Hua
• Xu Liguang
• Sun Maozhong
• Wu Xiaoling
• Liu Liqiang
• Ma Wei
• Zhu Jianping
• Hao Changlong
• Song Shanshan
• Hu Yongming
• Wu Aihong
• Guo Lingling
• Xu Xinxin

Classifications

• G01N33/9486

  Analgesics, e.g. opiates, aspirine · CPC title

• C07K2317/92

  Affinity (KD), association rate (Ka), dissociation rate (Kd) or EC50 value · CPC title

• G01N2470/10

  Competitive assay format · CPC title

• G01N33/577

  involving monoclonal antibodies {binding reaction mechanisms characterised by the use of monoclonal antibodies (G01N33/5302 - G01N33/576 take precedence)} · CPC title

• C07C231/12

  by reactions not involving the formation of carboxamide groups · CPC title