Methods and compositions for treating intestinal disorder

Patent metadata

Field	Value
Publication number	US-12501917-B2
Application number	US-202017425080-A
Country	US
Kind code	B2
Filing date	Jan 23, 2020
Priority date	Jan 23, 2019
Publication date	Dec 23, 2025
Grant date	Dec 23, 2025

Abstract

Provided are methods for determining responsiveness of a companion animal having an intestinal disorder to a diet. These methods include measuring at least a first amount of a first intestinal microorganism, comparing the first amount of the first intestinal microorganism with a first reference amount of the first intestinal microorganism, wherein the reference amount is determined based on the amounts of the intestinal microorganisms in a plurality of healthy companion animals; and determining that the companion animal is responsive to the diet, when the first amount of the intestinal microorganism is higher than the first reference amount of the first intestinal microorganism.

First claim

What is claimed is:

1 . A method for determining responsiveness of a companion animal having canine chronic enteritis to a diet, comprising:
a) administering the diet to the companion animal, wherein the diet comprises a food product comprising Clostridium hiranonis; b) measuring a first amount of a first intestinal microorganism, a second amount of a second intestinal microorganism, and a third amount of one or more secondary bile acids in the companion animal; c) comparing the first amount of the first intestinal microorganism with a first reference amount of the first intestinal microorganism, comparing the second amount of the second intestinal microorganism with a second reference amount of the second intestinal microorganism, and comparing the third amount of one or more secondary bile acids with a third reference amount of the one or more secondary bile acids, wherein the reference amounts of the intestinal microorganisms and one or more secondary bile acids are determined based on the amounts of the intestinal microorganisms and the one or more secondary bile acids in a plurality of healthy companion animals; d) determining that the companion animal is responsive to the diet, when the first amount of the intestinal microorganism is higher than the first reference amount of the first intestinal microorganism, when the second amount of the second intestinal microorganism is lower than the second reference amount of the second intestinal microorganism, and when the third amount of the one or more secondary bile acids is higher than the third reference amount of the one or more secondary bile acids, or determining that the companion animal is non-responsive to the diet, when the first amount of the intestinal microorganism is lower than the first reference amount of the first intestinal microorganism, when the second amount of the second intestinal microorganism is higher than the second reference amount of the second intestinal microorganism, and when the third amount of the one or more secondary bile acids is lower than the third reference amount of the one or more secondary bile acids.

2 . The method of claim 1 , wherein the first intestinal microorganism comprises one or more bacterium comprising a 16S rRNA comprising a nucleotide sequence that is at least about 90% homologous or identical to the 16S rRNA nucleotide sequence of SEQ ID NO:11, SEQ ID NO:8, SEQ ID NO:18, SEQ ID NO:9, SEQ ID NO:10, SEQ ID NO:27, SEQ ID NO:19, SEQ ID NO: 20, SEQ ID NO:15, SEQ ID NO:21, SEQ ID NO:28, SEQ ID NO:14, or SEQ ID NO:23.

3 . The method of claim 1 , wherein the second intestinal microorganism comprises one or more bacterium comprising a 16S rRNA comprising a nucleotide sequence that is at least about 90% homologous or identical to the 16S rRNA nucleotide sequence of SEQ ID NO:22, SEQ ID NO:24, SEQ ID NO:26, SEQ ID NO:13, SEQ ID NO:16, SEQ ID NO:25, SEQ ID NO:17, or SEQ ID NO:12.

4 . The method of claim 1 , further comprising administering the diet, a steroid and optionally an antibiotic to the companion animal when the companion animal is determined as non-responsive to the diet.

5 . The method of claim 1 , wherein the first intestinal microorganism comprises one or more bacterium comprising a 16S rRNA comprising a nucleotide sequence that is at least about 90% homologous or identical to a 16S rRNA nucleotide sequence selected from the group consisting of SEQ ID NO: 32 and SEQ ID NO: 33.

6 . The method of claim 1 , wherein the second intestinal microorganism comprises one or more bacterium comprising a 16S rRNA comprising a nucleotide sequence that is at least about 90% homologous or identical to a 16S rRNA nucleotide sequence selected from the group consisting of SEQ ID NOs: 29, 36, 38, 35, 48, 30, and 37.

7 . The method of claim 1 , wherein the method further comprises:
measuring a metabolic potential in the companion animal; and
determining that the companion animal is responsive to the diet, when the metabolic potential has shifted from lipid metabolism to carbohydrate metabolism, or determining that the companion animal is not responsive to the diet, when the metabolic potential has not shifted from lipid metabolism to carbohydrate metabolism.

8 . The method of claim 1 , wherein the second intestinal microorganism is Escherichia coli or Clostridium perfringens.

9 . The method of claim 1 , wherein the one or more secondary bile acids comprise at least one of deoxycholic acid and lithocholic acid.

Assignees

• Mars Inc
• Univ Pennsylvania

Inventors

• Perea Sally Christine
• Beiting Daniel P

Classifications

• G01N2800/52

  Predicting or monitoring the response to treatment, e.g. for selection of therapy based on assay results in personalised medicine; Prognosis · CPC title

• G01N2800/50

  Determining the risk of developing a disease · CPC title

• C12Q1/06

  Quantitative determination · CPC title

• A61K35/742

  Spore-forming bacteria, e.g. Bacillus coagulans, Bacillus subtilis, clostridium or Lactobacillus sporogenes · CPC title

• A61K31/575

  substituted in position 17 beta by a chain of three or more carbon atoms, e.g. cholane, cholestane, ergosterol, sitosterol · CPC title