Photoresponsive liposome, complex, measurement system, and measurement method

Patent metadata

Field	Value
Publication number	US-12498368-B2
Application number	US-202117461748-A
Country	US
Kind code	B2
Filing date	Aug 30, 2021
Priority date	Mar 1, 2019
Publication date	Dec 16, 2025
Grant date	Dec 16, 2025

Abstract

A complex includes photoresponsive liposomes, a first buffer solution, and a second buffer solution. Each of the photoresponsive liposomes includes a membrane that contains azobenzene compound and phospholipid and a membrane binding site that is immobilized to the membrane and bindable to a target substance. The first buffer solution is filled inside the photoresponsive liposomes. The second buffer solution exists outside of the photoresponsive liposomes. The first buffer solution has a composition that is different from that of the second buffer solution.

First claim

What is claimed is:

1 . A complex comprising:
a plurality of photoresponsive liposomes each including:
a membrane that contains azobenzene compound and phospholipid; and
a membrane binding site that is immobilized to the membrane and bindable to a target substance;
a first buffer solution that exists inside the plurality of photoresponsive liposomes; and a second buffer solution that exists outside of the plurality of photoresponsive liposomes, wherein the first buffer solution has a composition that is different from that of the second buffer solution.

2 . The complex according to claim 1 , wherein
the phospholipid includes a phospholipid having an unsaturated hydrocarbon chain and a phospholipid having a saturated hydrocarbon chain.

3 . The complex according to claim 1 wherein
the azobenzene compound includes azobenzene trimethylammonium bromide.

4 . The complex according to claim 1 , wherein
an ion-gradient exists between the first buffer solution and the second buffer solution.

5 . The complex according to claim 4 , wherein
the ion-gradient is a proton-gradient.

6 . A method for measuring an amount of a target substance, the method comprising:
applying a liquid that contains the target substance to a supporter to which a plurality of supporter binding sites are immobilized, each of the plurality of supporter binding sites being bindable to a first portion of the target substance; and adding the target substance to the complex according to claim 1 ; after applying the liquid that contains the target substance to the supporter and adding the target substance to the complex, removing an unbound photoresponsive liposome from the plurality of photoresponsive liposomes, the unbound photoresponsive liposome being a photoresponsive liposome that is not immobilized to the supporter; after removing the unbound photoresponsive liposome, forming a membrane pore in each of a remaining plurality of photoresponsive liposomes by irradiating the remaining plurality of photoresponsive liposomes with light in a state where the first buffer solution exists inside the remaining plurality of photoresponsive liposomes and the second buffer solution exists outside of the remaining plurality of photoresponsive liposomes; and then measuring the amount of the target substance in accordance with a change in the composition of the second buffer solution, the change being caused by the light irradiation.

7 . The method according to claim 6 , wherein
a proton-gradient exists between the first buffer solution and the second buffer solution before the light irradiation, and the amount of the target substance is measured in accordance with a change in pH of the second buffer solution caused by the light irradiation.

Assignees

• Denso Corp
• Japan Advanced Institute Of Science And Tech

Inventors

• Nukazuka Akira
• Kuno Hitoshi
• Hamada Tsutomu
• Kouda Katsunori
• Kobayashi Taira

Classifications

• G01N27/416

  Systems (G01N27/27 takes precedence) · CPC title

• G01N33/544 Primary

  the carrier being organic · CPC title

• G01N33/586 Primary

  Liposomes, microcapsules or cells · CPC title