Method for preparing kestose-containing fructooligosaccharides

Patent metadata

Field	Value
Publication number	US-12497639-B2
Application number	US-202017772721-A
Country	US
Kind code	B2
Filing date	Oct 30, 2020
Priority date	Oct 31, 2019
Publication date	Dec 16, 2025
Grant date	Dec 16, 2025

Abstract

The present invention relates to a method of preparing kestose-containing fructooligosaccharide, and more specifically, a method of preparing kestose-containing fructooligosaccharide having a high content of kestose and excellent storage stability.

First claim

The invention claimed is:

1 . A method of preparing a stable kestose-containing fructooligosaccharide, comprising
a step of performing a kestose conversion reaction using a substrate comprising sucrose and an enzyme having kestose conversion activity or a microorganism producing the enzyme; a step of terminating the kestose conversion reaction to obtain the reaction product of the kestose conversion reaction; a separation step of obtaining a kestose-containing fructooligosaccharide fraction from the reaction product of the kestose conversion reaction using simulated moving bed (SMB) chromatography, wherein the fraction comprises 80 wt % or more of kestose (GF2) based on 100 wt % of the saccharide solid content of the fraction, and a step of ion-purification performed by passing the kestose-containing fructooligosaccharide fraction through an ion purification column comprising a mixed bed column followed by completing with an anion exchange resin column, to obtain a stable kestose-containing fructooligosaccharide, wherein pH of the stable kestose-containing fructooligosaccharide is in the range of pH 5 to 8, wherein the step of terminating the kestose conversion reaction is performed when the sucrose content is 15 to 35 wt % and a nystose content is 0.01 to 5 wt % based on 100 wt % of the saccharide solid content of the reaction product, and wherein the stable kestose-containing fructooligosaccharide has a property of pH of 3.5 to 8 after storage at 45° C. for 7 weeks.

2 . The method according to claim 1 , wherein the step of performing a kestose conversion reaction is performed by using a substrate comprising 80 to 100 wt % of sucrose based on the saccharide solid content of the substrate comprising the sucrose.

3 . The method according to claim 1 , wherein the separating step is to obtain a fructooligosaccharide fraction comprising 90 wt % or more of kestose (GF2) based on 100 wt % of the saccharide solid content of the fructooligosaccharide fraction.

4 . The method according to claim 1 , wherein the separating step is to obtain a fructooligosaccharide fraction comprising 10 wt % or less of nystose (GF3) based on 100 wt % of the saccharide solid content of the kestose-containing fructooligosaccharide fraction.

5 . The method according to claim 1 , wherein the step of terminating the kestose conversion reaction is to produce the reaction product comprising 15 to 35 wt % of sucrose, 30 to 60 wt % of kestose, and 0.01 to 5 wt % of nystose, based on the saccharide solid content of the reaction product.

6 . The method according to claim 5 , wherein the step of terminating the kestose conversion reaction is to produce an enzyme reaction product comprising less than 25 wt % of glucose based on 100% saccharide solid content of the reaction product.

7 . The method according to claim 1 , wherein the separation step is applied to the kestose conversion reaction product having a solid content of 70 to 80 Brix.

8 . The method according to claim 1 , wherein the step of terminating the kestose conversion reaction is to terminate the kestose conversion reaction when the weight percent ratio (%) of the nystose (GF3) content to the total content of kestose (GF2) and nystose (GF2) of the reaction product calculated by Equation 1 below is 10% or less, based on 100 wt % of the saccharide solid content of the reaction product:

Nystose
⁢

weight
⁢

%

(

K
⁢
estose
⁢

weight
⁢

%

)

+

(

Nystose
⁢

weight
⁢

%

)

×
100
⁢

(
%
)

.

[

Equation
⁢

1

]

9 . The method according to claim 1 , wherein the step of terminating the kestose conversion reaction is to terminate the kestose conversion reaction so that the weight percent ratio (%) of the nystose (GF3) content to the total content of kestose (GF2) and nystose (GF2) of the kestose-containing frauctooligosachcaride fraction calculated by Equation 1 below is 15% or less, based on 100 wt % of the saccharide solid content of the kestose-containing fructooligosaccharide fraction obtained by separating from the reaction product of the kestose conversion reaction:

Nystose
⁢

weight
⁢

%

(

K
⁢
estose
⁢

weight
⁢

%

)

+

(

Nystose
⁢

weight
⁢

%

)

×
100
⁢

(
%
)

.

[

Equation
⁢

Assignees

• Samyang Corp

Inventors

• Park Ji Won
• Kim Go-Eun
• Nam Choong Woo
• Park Chong Jin
• Yang Jae-Kyung
• Han Jung Sook

Classifications

• C12Y302/01026

  Beta-fructofuranosidase (3.2.1.26), i.e. invertase · CPC title

• C12N9/2431

  Beta-fructofuranosidase (3.2.1.26), i.e. invertase · CPC title

• B01D15/363

  Anion-exchange · CPC title

• B01D15/362

  Cation-exchange · CPC title

• B01D15/185

  characterised by the components to be separated · CPC title