Method for increased throughput

Patent metadata

Field	Value
Publication number	US-12494357-B2
Application number	US-202117999641-A
Country	US
Kind code	B2
Filing date	May 21, 2021
Priority date	May 22, 2020
Publication date	Dec 9, 2025
Grant date	Dec 9, 2025

Abstract

A trace of intensity versus time values is received for a series of samples produced by a mass spectrometer. Also, a series of ejections times corresponding to the series of samples produced by a sample introduction system is received. A series of expected peak times corresponding to the series of ejection times are calculated using a known delay time from ejection to mass analysis. At least one isolated peak of the trace is identified using the series of expected peak times. A peak profile is calculated by fitting a mixture of at least two different distribution functions to the at least one isolated peak. For at least one time of the series of expected peak times, an area of a peak at the one time is calculated by fitting the peak profile to the trace at the one time and calculating an area of the fitted peak profile.

First claim

What is claimed is:

1 . A system for calculating an area of a sample peak of a trace produced using high-throughput sample introduction coupled mass spectrometry, comprising:
a sample introduction system that ejects each sample of a series of samples at an ejection time, producing a series of ejections times corresponding to the series of samples, and ionizes each ejected sample of the series of samples, producing an ion beam; a mass spectrometer that receives the ion beam and mass analyzes the ion beam over time, producing a trace of intensity versus time values for one or more mass-to-charge ratio (m/z) values for the series of samples; and a processor that
receives the trace and the series of ejection times,
calculates a series of expected peak times corresponding to the series of ejection times using a known delay time from ejection to mass analysis,
identifies at least one isolated peak of the trace using the series of expected peak times,
calculates a peak profile by fitting a mixture of at least two different distribution functions to the at least one isolated peak, and
for at least one time of the series of expected peak times, calculates an area of a peak at the at least one time by fitting the peak profile to the trace at the one time and calculating an area of the fitted peak profile.

2 . The system of claim 1 , wherein the processor identifies at least one isolated peak of the trace using the series of expected peak times by:
identifying one or more peaks that has a minimum overlap with adjacent peaks by calculating intensities at midpoints between peaks using the series of expected peak times and selecting each peak that has an intensity at each midpoint with an adjacent peak that is less than a threshold intensity value, and identifying a peak of the one or more peaks that has a minimum overlap and that has a highest intensity as the at least one isolated peak.

3 . The system of claim 1 , wherein the at least two different distribution functions comprise a Gaussian distribution function.

4 . The system of claim 1 , wherein the at least two different distribution functions comprise a Weibull distribution function.

5 . The system of claim 1 , wherein the sample introduction system comprises a surface analysis system.

6 . The system of claim 5 , wherein the surface analysis system comprises a matrix-assisted laser desorption/ionization (MALDI) device.

7 . The system of claim 5 , wherein the surface analysis system comprises a laser diode thermal desorption (LDTD) device.

8 . The system of claim 1 , wherein the sample introduction system comprises a flow injection device and an ion source device.

9 . The system of claim 8 , wherein the flow injection device comprises a timed valve device that injects sample into a flowing stream through a valve at each ejection time of the series of ejection times and wherein the ion source device ionizes samples of the flowing stream, producing the ion beam.

10 . The system of claim 8 , wherein the flow injection device comprises a droplet dispenser that ejects the series of samples as droplets into a flowing stream at each ejection time of the series of ejection times and wherein the ion source device ionizes samples of the flowing stream, producing the ion beam.

11 . The system of claim 10 , wherein the droplet dispenser comprises an acoustic droplet ejection (ADE) device that ejects the series of samples as droplets into an inlet of a tube of an open port interface (OPI), wherein the OPI mixes the droplets of the series of samples with a solvent in the tube to form a series of analyte-solvent dilutions and transfers the series of analyte-solvent dilutions to an outlet of the tube of the OPI, and wherein the ion source device receives the series of dilutions and ionizes samples of the series of dilutions, producing the ion beam.

12 . The system of claim 1 , wherein each time of the series of expected peak times comprises a time at which an apex of a peak is expected.

13 . The system of claim 1 , wherein the mixture of at least two different distribution functions produces an asymmetric peak that has a larger leading edge gradient than a trailing edge gradient.

14 . A method for calculating the area of a sample peak of a trace produced using high-throughput sample introduction coupled mass spectrometry, comprising:
receiving a trace of intensity versus time values for one or more mass-to-charge ratio (m/z) values for a series of samples produced by a mass spectrometer and a series of ejections times corresponding to the series of samples produced by a sample introduction system using a processor; calculating a series of expected peak times corresponding to the series of ejection times using a known delay time from ejection to mass analysis using the processor; identifying at least one isolated peak of the trace using the series of expected peak times using the processor; calculating a peak profile by fitting a mixture of at least two different distribution functions to the at least one isolated peak using the processor; and for at least one time of the series of expected peak times, calculating an area of a peak at the one time by fitting the peak profile to the trace at the one time and calculating an area of the fitted peak profile using the processor.

15 . A computer program product, comprising a non-transitory and tangible computer-readable storage medium whose contents include a program with instructions being executed on a processor so as to perform a method for calculating the area of a sample peak of a trace produced using high-throughput sample introduction coupled mass spectrometry, the method comprising:
providing a system, wherein the system comprises one or more distinct software modules, and wherein the one or more distinct software modules comprise an analysis module; receiving a trace of intensity versus time values for one or more mass-to-charge ratio (m/z) values for a series of samples produced by a mass spectrometer and a series of ejections times corresponding to the series of samples produced by a sample introduction system using the analysis module; calculating a series of expected peak times corresponding to the series of ejection times using a known delay time from ejection to mass analysis using the analysis module; identifying at least one isolated peak of the trace using the series of expected peak times using the analysis module; calculating a peak profile by fitting a mixture of at least two different distribution functions to the at least one isolated peak using the analysis module; and for at least one time of the series of expected peak times, calculating an area of a peak at the one time by fitting the peak profile to the trace at the one time and calculating an area of the fitted peak profile using the analysis module.

Assignees

• Dh Technologies Dev Pte Ltd

Inventors

• Covey Thomas R
• Ivosev Gordana
• Kovarik Peter
• Liu Chang

Classifications

• H01J49/0418

  for laser desorption, e.g. matrix-assisted laser desorption/ionisation [MALDI] plates or surface enhanced laser desorption/ionisation [SELDI] plates · CPC title

• H01J49/0036 Primary

  Step by step routines describing the handling of the data generated during a measurement · CPC title

• H01J49/0454 Primary

  with means for vaporising using mechanical energy, e.g. by ultrasonic vibrations · CPC title

• H01J49/0031

  Step by step routines describing the use of the apparatus (H01J49/0081 takes precedence) · CPC title