Methods of use of seed-origin endophyte populations

Patent metadata

Field	Value
Publication number	US-12490744-B2
Application number	US-202318368704-A
Country	US
Kind code	B2
Filing date	Sep 15, 2023
Priority date	Jun 26, 2013
Publication date	Dec 9, 2025
Grant date	Dec 9, 2025

Abstract

This application relates to methods and materials for providing a benefit to a seed or seedling of an agricultural plant (e.g., an agricultural grass plant), or the agricultural plant derived from the seed or seedling. For example, this application provides purified bacterial populations that include novel seed-origin bacterial endophytes, and synthetic combinations of seeds and/or seedlings (e.g., cereal seeds and/or seedlings) with heterologous seed-derived bacterial endophytes.

First claim

What is claimed is:

1 . A method of treating a cereal agricultural plant, comprising mechanically or manually contacting a seed of a cereal agricultural plant with an agricultural formulation comprising an agriculturally compatible carrier and a purified bacterial population, wherein the purified bacterial population comprises an Enterobacteriaceae seed bacterial endophyte that is heterologous to the seed, has a 16S nucleic acid sequence that is at least 97% identical to the 16S nucleic acid sequence of SEQ ID NO: 815, wherein the purified bacterial population is present in the formulation in an amount capable of increasing one or more of root length and seedling weight of an agricultural plant grown from the seed under drought stress.

2 . The method of claim 1 , wherein the contacting comprises spraying, immersing, coating, encapsulating, or dusting the cereal agricultural plant with the formulation.

3 . The method of claim 1 , wherein the cereal agricultural plant is corn, wheat, rice, or barley.

4 . The method of claim 1 , wherein the bacterial endophyte is present at a concentration of at least 10{circumflex over ( )}2 CFU/seed on the surface of the seed of the cereal agricultural plant.

5 . The method of claim 1 , wherein the agriculturally compatible carrier comprises one or more of: a mineral carrier, a liquid carrier, a tackifier, a surfactant, a microbial stabilizer, a fungicide, an antibacterial agent, an herbicide, a nematicide, an insecticide, a plant growth regulator, a rodenticide, a desiccant, and a nutrient.

6 . The method of claim 5 , wherein the agriculturally compatible carrier comprises a mineral carrier and the mineral carrier is selected from the group consisting of kaolin clay, pyrophyllite, bentonite, montmorillonite, diatomaceous earth, acid white soil, vermiculite, and pearlite, and inorganic salts.

7 . The method of claim 1 , wherein the agricultural formulation additionally comprises a vegetable oil or mineral oil.

8 . The method of claim 1 , wherein the bacterial endophyte is capable of phosphate solubilization.

9 . A synthetic combination comprising a purified bacterial population in association with a seed of a cereal agricultural plant, wherein the purified bacterial population comprises an Enterobacteriaceae seed bacterial endophyte that is heterologous to the seed, has the 16S nucleic acid sequence that is at least 97% identical to a 16S nucleic acid sequence of SEQ ID NO: 815, wherein the bacterial endophyte is present in the synthetic combination at a concentration of at least 10{circumflex over ( )}3 CFU/seed on the surface of a seed that is effective for increasing one or more of root length and seedling weight of an agricultural plant grown from the seed under drought stress.

10 . A synthetic combination comprising a seed of a cereal agricultural plant and a purified bacterial population in association with an agriculturally compatible carrier, wherein the purified bacterial population comprises an Enterobacteriaceae seed bacterial endophyte that is heterologous to the seed, has a 16S nucleic acid sequence that is at least 97% identical to the 16S nucleic acid sequence of SEQ ID NO: 815, wherein the agriculturally compatible carrier comprises one or more of talc, an oil, kaolin clay, a dispersant, a surfactant, and a nutrient, wherein the purified bacterial population is present in an amount capable of increasing one or more of root length and seedling weight of a cereal agricultural plant grown from the seed under drought stress.

11 . The synthetic combination of claim 10 , wherein the synthetic combination is disposed within a packaging material selected from a bag, box, bin, envelope, carton, or container.

12 . The synthetic combination of claim 11 , wherein the packaging material additionally comprises a desiccant.

13 . The synthetic combination of claim 9 , wherein the purified bacterial population is localized on the surface of the seeds.

14 . The synthetic combination of claim 13 , wherein the localization on the surface is a substantially uniform coating on the seeds.

15 . The synthetic combination of claim 10 , wherein the bacterial endophyte is capable of phosphate solubilization.

16 . The synthetic combination of claim 10 , wherein the bacterial endophyte is shelf stable.

17 . The synthetic combination of claim 10 , wherein the oil is a vegetable oil or mineral oil.

18 . The method of claim 6 , wherein the inorganic salts comprise one or more of ammonium sulfate ammonium phosphate, ammonium nitrate, urea, ammonium chloride, and calcium carbonate.

Assignees

• Indigo Ag Inc

Inventors

• Von Maltzahn Geoffrey
• Flavell Richard Bailey
• Toledo Gerardo V
• Djonovic Slavica
• Marquez Luis Miguel
• Johnston David Morris
• Millet Yves Alain
• Lyford Jeffrey
• Naydich Alexander
• Sadowski Craig

Classifications

• G01N33/5097

  involving plant cells (immunoassays of plant cells G01N33/56961) · CPC title

• A01C1/06 Primary

  Coating or dressing seed · CPC title

• A01C1/00 Primary

  Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting · CPC title

• A01N63/20 Primary

  Bacteria; Substances produced thereby or obtained therefrom · CPC title

• A01N25/00

  Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application {, e.g. seed treatment or sequential application}; Substances for reducing the noxious effect of the active ingredients to organisms other than pests · CPC title