Molecular markers for germplasm identification and selection of carotenoid-rich bay scallops

Patent metadata

Field	Value
Publication number	US-12486543-B2
Application number	US-202418912947-A
Country	US
Kind code	B2
Filing date	Oct 11, 2024
Priority date	Dec 1, 2023
Publication date	Dec 2, 2025
Grant date	Dec 2, 2025

Abstract

Embodiments of the present disclosure provide molecular markers for germplasm identification and selection of carotenoid-rich bay scallops. The molecular markers include SNP1, SNP2, SNP3 and SNP4. Embodiments of the present disclosure also provide a group of primers as shown in SEQ ID NO. 5, SEQ ID NO. 6 and SEQ ID NO. 7, SEQ ID NO. 8 for the molecular markers. The disclosure provides a rapid, simple, and batch method for the identification and screening of carotenoid-rich bay scallops at a seedling stage and an adult scallop stage, which can effectively reduce the cultivation cost of carotenoid-rich scallops, improve the efficiency of farming and breeding, and has a broad market application prospect.

First claim

What is claimed is:

1 . A method for screening carotenoid-rich bay scallops based on molecular markers, wherein the method comprises the following steps:
(1) sampling bay scallops to be tested; (2) extracting sample DNA from the bay scallops to be tested as a template, using nucleotide sequences shown in SEQ ID NOs. 5-6 and 7-8 as primers to separately perform PCR amplification on the sample DNA, obtaining PCR amplification products, and sequencing the PCR amplification products to determine a genotyping type of SNP1, SNP2, SNP3 and SNP4 sites in each bay scallop to be tested; (3) when the genotyping result exhibits that a genotype of one of the bay scallops to be tested containing the SNP1 site is of a GG type, a genotype of the bay scallop to be tested containing the SNP2 site is of a TT type, a genotype of the bay scallop to be tested containing the SNP3 site is of an AA type, or a genotype of the bay scallop to be tested containing the SNP4 site is of a CC type, determining the bay scallop to be tested as a carotenoid-rich bay scallop; wherein the SNP1 site is located at position 26 of a nucleotide sequence shown in SEQ ID NO. 1, and the base of the SNP1 site is T or G; the SNP2 site is located at position 27 of the nucleotide sequence shown in SEQ ID NO. 2, and the base of the SNP2 site is G or T; the SNP3 site is located at position 172 of the nucleotide sequence shown in SEQ ID NO. 3, and the base of the SNP3 site is C or A; the SNP4 site is located at position 45 of a nucleotide sequence shown in SEQ ID NO. 4, and the base of the SNP4 site is T or C.

2 . The method of claim 1 , wherein in the step (1), different sampling strategies are adopted at different time periods, and the sampling strategies include:
in a small-size seedling stage, randomly selecting 50-100 bay scallops from a bay scallop population and performing whole tissue sampling for the identification of the seeds of the bay scallop; in a large-size seedling stage, taking 3-5 gill filaments of each bay scallop and performing non-destructive sampling on the gill filaments for the directed selection of the seeds of the bay scallop; and in an adult scallop stage, taking 3-5 gill filaments of each bay scallop and performing non-destructive sampling on the gill filaments for the selection of parents of bay scallop.

Assignees

• Ocean Univ China

Inventors

• Hu Xiaoli
• Liu Shiqi
• Wang Huizhen
• Zhang Yihan
• Yuan Jingyao
• Bao Zhenmin

Classifications

• C12Q2600/124

  Animal traits, i.e. production traits, including athletic performance or the like · CPC title

• C12Q1/686 Primary

  Polymerase chain reaction [PCR] · CPC title

• C12Q2600/156

  Polymorphic or mutational markers · CPC title

• C12Q1/6895

  for plants, fungi or algae · CPC title

• C12Q1/6888 Primary

  for detection or identification of organisms · CPC title