Enzymatic method for producing l-glufosinate and its phosphoesters
US-2024117327-A1 · Apr 11, 2024 · US
L-arginine-glycine amidinotransferase and use thereof in the production of guanidinoacetic acid
US12486498B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-12486498-B2 |
| Application number | US-202418415827-A |
| Country | US |
| Kind code | B2 |
| Filing date | Jan 18, 2024 |
| Priority date | Mar 20, 2023 |
| Publication date | Dec 2, 2025 |
| Grant date | Dec 2, 2025 |
How to read this patent
A practical reading order for non-experts. Skip the full description unless you need deep technical detail.
- Title
What the patent document calls the invention.
- Abstract
A short plain-language summary of the technical disclosure.
- Assignees and inventors
Who owns or filed the patent and who is credited as inventor.
- Key dates
Filing, priority, publication, and grant dates set the timeline.
- First independent claim
The legal scope of protection — read this for what is actually claimed.
- CPC / IPC classifications
Technology tags used to group this patent with similar filings.
- Citations and related patents
Prior art links and similar publications in this corpus.
Abstract
Official abstract text for this publication.
The present invention discloses an L-arginine-glycine amidinotransferase and use thereof in the production of guanidinoacetic acid. In the present invention, through combined multi-site amino acid mutation, a technical effect of significantly improved enzyme activity of the mutant AkAGAT T225Q/A258P/L278K than that of the wild-type strain is achieved, providing an application value for large-scale production of guanidinoacetic acid in industry. When the L-arginine-glycine amidinotransferase mutant constructed in the present invention is used in the production of guanidinoacetic acid, by optimizing the conversion conditions, the yield of guanidinoacetic acid is up to 21.4 g/L and the conversion rate is 90.4%, after 24 hrs of reaction in a 1 L reaction system. Compared with the production of guanidinoacetic acid with the raw enzyme, the yield is increased by 49.6%.
First claim
Opening claim text (preview).
What is claimed is: 1 . An L-arginine-glycine amidinotransferase comprising all of SEQ ID NO: 4 except for (i) a glutamine at the position corresponding to position 225 of the polypeptide of SEQ ID NO: 4; (ii) a glutamine at the position corresponding to position 225 of the polypeptide of SEQ ID NO: 4, and a proline at the position corresponding to position 258 of the polypeptide of SEQ ID NO: 4, or (iii) a glutamine at the position corresponding to position 225 of the polypeptide of SEQ ID NO: 4, a proline at the position corresponding to position 258 of the polypeptide of SEQ ID NO: 4, and a lysine at the position corresponding to position 278 of the polypeptide of SEQ ID NO: 4.
Assignees
Inventors
Classifications
Glycine amidinotransferase (2.1.4.1) · CPC title
- C12P13/04Primary
Alpha- or beta- amino acids {(other amino acids C12P13/005)} · CPC title
Vectors or expression systems specially adapted for E. coli · CPC title
Citrulline; Arginine; Ornithine · CPC title
transferring nitrogenous groups (2.6) · CPC title
Patent family
Related publications grouped by family.
External sources
Frequently asked questions
Answers are generated from the same data shown on this page.
- What does patent US12486498B2 cover?
- The present invention discloses an L-arginine-glycine amidinotransferase and use thereof in the production of guanidinoacetic acid. In the present invention, through combined multi-site amino acid mutation, a technical effect of significantly improved enzyme activity of the mutant AkAGAT T225Q/A258P/L278K than that of the wild-type strain is achieved, providing an application value for large-s…
- Who is the assignee on this patent?
- Univ Jiangnan
- What technology area does this patent fall under?
- Primary CPC classification C12P13/04. Mapped technology areas include Chemistry & Metallurgy.
- When was this patent published?
- Publication date Tue Dec 02 2025 00:00:00 GMT+0000 (Coordinated Universal Time) (B2). Legal status and post-grant events are not shown on this page.
- What related patents are in patentsdb?
- We list 8 related publications on this page (citations in our corpus or others sharing the same primary CPC).