Microorganism of Corynebacterium genus having enhanced L-arginine or L-citrulline productivity and a method for producing L-arginine or L-citrulline using the same

Patent metadata

Field	Value
Publication number	US-12486490-B2
Application number	US-202217937833-A
Country	US
Kind code	B2
Filing date	Oct 4, 2022
Priority date	Jul 21, 2022
Publication date	Dec 2, 2025
Grant date	Dec 2, 2025

Abstract

The present disclosure relates to a Corynebacterium sp. mutant strain having increased L-arginine or L-citrulline productivity and a method of producing L-arginine or L-citrulline using the same. The Corynebacterium sp. mutant strain has enhanced activity of acetylornithine aminotransferase involved in the L-arginine biosynthesis pathway, and thus is capable of producing L-arginine or L-citrulline productivity in an increased yield compared to a parent strain.

First claim

What is claimed is:

1 . A Corynebacterium sp. mutant strain having increased L-arginine or L-citrulline productivity by having enhanced activity of acetylornithine aminotransferase,
wherein said acetylornithine aminotransferase has a substitution of threonine for alanine at amino acid position 131 in the amino acid sequence of SEQ ID NO: 2.

2 . The Corynebacterium sp. mutant strain of claim 1 , which is Corynebacterium glutamicum.

3 . A method for producing L-arginine or L-citrulline comprising steps of:
culturing the Corynebacterium sp. mutant strain of claim 1 in a medium; and recovering L-arginine or L-citrulline from the cultured mutant strain or the medium in which the mutant strain has been cultured.

Assignees

• Daesang Corp

Inventors

• Ryu Mi
• Yoon Sun Jun
• Hong In Pyo
• Park Seok Hyun

Classifications

• C12R2001/15

  Corynebacterium · CPC title

• C12Y206/01011

  Acetylornithine transaminase (2.6.1.11) · CPC title

• C12P13/10

  Citrulline; Arginine; Ornithine · CPC title

• C12N9/1096 Primary

  transferring nitrogenous groups (2.6) · CPC title

• C12N15/77

  for Corynebacterium; for Brevibacterium · CPC title