Process and polymorphic forms of bictegravir and its pharmaceutically acceptable salts or co-crystals thereof

Patent metadata

Field	Value
Publication number	US-12486286-B2
Application number	US-202017629985-A
Country	US
Kind code	B2
Filing date	Jun 18, 2020
Priority date	Jun 18, 2019
Publication date	Dec 2, 2025
Grant date	Dec 2, 2025

Abstract

The present invention generally relates to polymorphic forms of bictegravir, its salts, co-crystals, solvates or hydrates thereof and process for the preparation of the same and also relates to pharmaceutical compositions containing the same. The present invention also relates to a process for preparation of bictegravir substantially free from its diastereomer impurity.

First claim

We claim:

1 . A process for the preparation of bictegravir of Formula I:

comprising:
a) reacting a compound of Formula II with a compound of Formula III in the presence of at least one suitable activation agent and at least one suitable base in at least one suitable solvent to obtain a compound of Formula IV:

and
b) demethylating the compound of Formula IV in the presence of at least one suitable deprotecting agent to obtain the bictegravir of Formula I;
wherein the at least one suitable activation agent in step a) is selected from the group consisting of 2-chloro-4,6-dimethoxy-1,3,5-triazine, carbonyldiimidazole, 1-ethyl-3-(3-dimethyl amino propyl)carbodiimide hydrochloride, 1-hydroxybenzotriazole, 3-[bis(dimethylamino)methyliumyl]-3H-benzotriazol-1-oxide hexafluorophosphate, O-(1H-6-Chlorobenzotriazole-1-yl)-1,1,3,3-tetramethyluronium hexafluoro phosphate, O-(benzotriazol-1-yl)-N,N,N′,N′-tetramethyluronium tetrafluoroborate, propyl phosphonic anhydride, dicyclohexylcarbodiimide, 3-hydroxytriazolo[4,5-b]pyridine, thionyl chloride, oxalyl chloride, phosphorus oxychloride, pivaloyl chloride, acetic anhydride, N-ethoxycarbonyl-2-ethoxy-1,2-dihydroquinoline, methanesulfonyl chloride and p-toluenesulfonyl chloride, and any combination thereof,
wherein the at least one suitable base is selected from the group consisting of sodium hydroxide, potassium hydroxide, sodium amide, sodium methoxide, sodium ethoxide, sodium t-butoxide, potassium t-butoxide, sodium carbonate, potassium carbonate, cesium carbonate, sodium bicarbonate, potassium bicarbonate, triethylamine, diisopropylamine, diisopropyl ethylamine, N-methyl piperidine, N-ethylpiperidine, and any combination thereof,
wherein the at least one suitable solvent is selected from the group consisting of acetone, methyl isobutyl ketone, methyl ethyl ketone, ethylacetate, isopropyl acetate, butyl acetate, acetonitrile, propionitrile, tetrahydrofuran, dimethyl ether, diisopropyl ether, methyl tertiary butyl ether, 1,4-dioxane, methylene chloride, ethylene chloride, chloroform, toluene, xylene, dimethylacetamide, dimethylformamide, dimethylsulfoxide, N-methylpyrrolidone, and any combination thereof, and
wherein the at least one suitable deprotecting agent is selected from the group consisting of magnesium chloride, magnesium bromide, magnesium iodide, magnesium hydroxide, lithium chloride, lithium bromide, lithium iodide, lithium hydroxide, lithium trisiamyl borohydride, lithium triethyl borohydride, tri-sec-butyl borohydride, boron tribromide, aluminium chloride-triethylamine complex, aluminium chloride-N,N-dimethyl aniline complex, and any combination thereof.

2 . The process as claimed in claim 1 , further comprising purifying the isolated bictegravir of Formula I to reduce the content of diastereomeric impurities by:
c) suspending or dissolving the bictegravir of Formula I obtained in step b) in at least one suitable solvent selected from the group consisting of an alcohol, an ester, a halogenated hydrocarbon, and any combination thereof, d) separating undissolved solids from the step c) suspension or solution, and e) isolating the purified bictegravir of Formula I.

3 . The process as claimed in claim 2 , wherein the at least one suitable solvent is selected from the group consisting of methanol, ethanol, isopropanol, n-propanol, tert-butanol, ethyl acetate, isopropyl acetate, isobutyl acetate, methylene chloride, ethylene chloride, and any combination thereof.

4 . The process as claimed in claim 2 , wherein step d) is carried out by filtration or decantation.

5 . The process as claimed in claim 2 , wherein the bictegravir of Formula I of step c) has a content of diastereomeric impurity of more than 0.15% by HPLC and the bictegravir of Formula I of step e) has a content of diastereomeric impurity of less than 0.15% by HPLC.

6 . The process according to claim 1 , further comprising forming a pharmaceutical composition by combining the bictegravir of Formula I obtained in step b), and at least one pharmaceutically acceptable excipient.

7 . A crystalline bictegravir sodium Form-L characterized by X-Ray diffraction (XRD) pattern having one or more peaks at about 6.5, 9.1, 11.1, 11.3, 12.1, 12.9, 13.1, 14.0, 15.2, 15.6, 15.9, 16.5, 16.8, 17.2, 18.2, 18.8, 19.4, 19.9, 20.5, 21.1, 21.7, 22.6, 23.2, 24.3, 24.9, 25.6, 25.7, 26.5, 26.8, 27.4, 28.4, 29.6, 30.8, 31.4, 32.8, 34.5 and 35.1±0.2° 2θ.

8 . A process for the preparation of bictegravir sodium Form-L, comprising:
a) suspending or dissolving bictegravir in methylene chloride or trifluoroethanol at 25° C. to reflux temperature; b) optionally cooling the step a) reaction mass to below 25° C.; c) adding at least one sodium source to the step a) or step b) reaction mass or vice-versa, wherein the sodium source is selected from the group consisting of aqueous sodium hydroxide, an alcoholic sodium hydroxide, and a sodium alkoxide, the alcohol is selected from the group consisting of methanol, ethanol, butanol, isobutanol, propanol and isopropanol, and the sodium alkoxide is selected from the group consisting of sodium methoxide, sodium ethoxide, sodium butoxide and sodium pentoxide; and d) isolating the bictegravir sodium Form-L, wherein the isolated bictegravir sodium Form-L is characterized by an X-Ray diffraction (XRD) pattern having one or more peaks at about 6.5, 9.1, 11.1, 11.3, 12.1, 12.9, 13.1, 14.0, 15.2, 15.6, 15.9, 16.5, 16.8, 17.2, 18.2, 18.8, 19.4, 19.9, 20.5, 21.1, 21.7, 22.6, 23.2, 24.3, 24.9, 25.6, 25.7, 26.5, 26.8, 27.4, 28.4, 29.6, 30.8, 31.4, 32.8, 34.5 and 35.1±0.2° 2θ.

9 . The process as claimed in claim 8 , wherein the at least one sodium source is selected from the group consisting of methanolic sodium hydroxide, aqueous sodium hydroxide, and a combination thereof thereof.

10 . A crystalline bictegravir sodium Form-L1 characterized by X-Ray diffraction (XRD) pattern having one or more peaks at about 5.6, 6.2, 7.7, 9.1, 11.3, 11.9, 12.6, 12.8, 13.9, 15.4, 16.2, 16.8, 17.0, 17.6, 18.4, 18.8, 18.9, 19.2, 19.8, 20.5, 20.7, 21.0, 21.5, 22.8, 23.8, 24.2, 24.5, 25.3, 25.7, 27.1, 28.4, 28.8, 29.5, 30.0, 30.9, 31.3, 31.8, 32.9, 33.4, 34.0, and 34.9±0.2° 2θ.

11 . A process for the preparation of bictegravir sodium Form-L1, comprising:
a) suspending or dissolving bictegravir in a mixture of isopentanol and acetonitrile at 25° C. to reflux temperature; b) optionally cooling the step a) reaction mass to below 25° C.; c) adding a sodium source to step a) or step b) reaction mass or vice-versa;
wherein
the sodium source is selected from the group consisting of aqueous sodium hydroxide, an alcoholic sodium hydroxide, and a sodium alkoxide, wherein
the alcohol is selected from the group consisting of methanol, ethanol, butanol, isobutanol, propanol and isopropanol, and wherein
the sodium alkoxide is selected from the group consisting of sodium methoxide, sodium ethoxide, sodium butoxide and sodium pentoxide, and
d) isolating the bictegravir sodium Form-L1, wherein the isolated bictegravir sodium Form-L1 is characterized by an X-Ray diffraction (XRD) pattern having one or more peaks at about 5.6, 6.2, 7.7, 9.1, 11.3, 11.9, 12.6, 12.8, 13.9, 15.4, 16.2, 16.8, 17.0, 17.6, 18.4, 18.8, 18.9, 19.2, 19.8, 20.5, 20.7, 21.0, 21.5, 22.8, 23.8, 24.2, 24.5, 25.3, 25.7, 27.1, 28.4, 28.8, 29.5, 30.0, 30.9, 31.3, 31.8, 32.9, 33.4, 34.0, and 34.9±0.2° 2θ.

12 . The process as claimed in claim 11 , wherein the sodium source is aqueous sod

Assignees

• Laurus Labs Ltd

Inventors

• Thaimattam Ram
• Radhakrishnan Sureshbabu
• Seethamraju Pavan Kumar
• Beeravalli Satheesh
• Pampana Uma Maheswara Rao
• Shamakura Anil Kumar Reddy
• Bollu Ravindra Babu
• Vasireddi Uma Maheswer Rao

Classifications

• C07B2200/13

  Crystalline forms, e.g. polymorphs · CPC title

• C07D498/18 Primary

  Bridged systems · CPC title

• A61P31/18

  for HIV · CPC title

• A61K31/553 Primary

  having at least one nitrogen and one oxygen as ring hetero atoms, e.g. loxapine, staurosporine · CPC title