Biosynthetic production of gamma- or delta-lactones using cytochrome p450 hydroxylase enzymes or mutants thereof
US-2024327368-A1 · Oct 3, 2024 · US
Biosynthetic production of gamma-lactones
US12480147B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-12480147-B2 |
| Application number | US-202218062628-A |
| Country | US |
| Kind code | B2 |
| Filing date | Dec 7, 2022 |
| Priority date | Jul 17, 2018 |
| Publication date | Nov 25, 2025 |
| Grant date | Nov 25, 2025 |
How to read this patent
A practical reading order for non-experts. Skip the full description unless you need deep technical detail.
- Title
What the patent document calls the invention.
- Abstract
A short plain-language summary of the technical disclosure.
- Assignees and inventors
Who owns or filed the patent and who is credited as inventor.
- Key dates
Filing, priority, publication, and grant dates set the timeline.
- First independent claim
The legal scope of protection — read this for what is actually claimed.
- CPC / IPC classifications
Technology tags used to group this patent with similar filings.
- Citations and related patents
Prior art links and similar publications in this corpus.
Abstract
Official abstract text for this publication.
Provided herein are methods for making gamma lactones comprising reacting a carboxylic acid substrate with a heterologous cytochrome P450 (CYP450) protein with carboxylic acid 4-hydroxylase activity.
First claim
Opening claim text (preview).
The invention claimed is: 1 . A method for producing a C4-C20 gamma lactone, the method comprising: providing a reaction mixture comprising a recombinant CYP450 protein having carboxylic acid 4-hydroxylase activity and a C4-C20 carboxylic acid substrate to provide a 4-hydroxy C4-C20 carboxylic acid; and subjecting the 4-hydroxy C4-C20 carboxylic acid to acidic conditions to produce the C4-C20 gamma lactone; wherein the recombinant CYP450 protein comprises an amino acid sequence having at least 90% identity to SEQ ID NO: 3 or SEQ ID NO: 5. 2 . The method of claim 1 , wherein the recombinant CYP450 protein is expressed by an isolated transformed host cell in the reaction mixture. 3 . The method of claim 2 , wherein the isolated transformed host cell is selected from a bacterial cell and a yeast cell. 4 . The method of claim 1 , wherein the recombinant CYP450 protein (a) comprises an amino acid sequence having at least 95% identity to SEQ ID NO: 3; (b) comprises an amino acid sequence having at least 98% identity to SEQ ID NO: 3; or (c) comprises the amino acid sequence of SEQ ID NO: 3. 5 . The method claim 1 , wherein the recombinant CYP450 protein (a) comprises an amino acid sequence having at least 95% identity to SEQ ID NO: 5; (b) comprises an amino acid sequence having at least 98% identity to SEQ ID NO: 5; or (c) comprises the amino acid sequence of SEQ ID NO: 5. 6 . The method of claim 1 , wherein the C4-C20 carboxylic acid substrate comprises a C4-C20 carboxylic acid; a salt of a C4-C20 carboxylic acid; an ester of a C4-C20 carboxylic acid; a mono-, di- or triglyceride of a C4-C20 carboxylic acid; or a combination thereof. 7 . The method of claim 1 , wherein the C4-C20 carboxylic acid comprises a straight-chain C4-C20 carboxylic acid, a branched C4-C20 carboxylic acid, a saturated C4-C20 carboxylic acid, an unsaturated C4-C20 carboxylic acid, or a combination thereof. 8 . The method of claim 4 , for producing a C4-C20 gamma lactone selected from γ-undecalactone and γ-undecenolactone, wherein the C4-C20 carboxylic acid substrate is selected from undecanoic acid and undecenoic acid. 9 . The method of claim 5 , for producing a C4-C20 gamma lactone selected from γ-hexalactone, γ-heptalactone, γ-decalactone, γ-undecalactone, γ-dodecalactone, γ-tridecalactone, and γ-tetradecalactone, wherein the C4-C20 carboxylic acid substrate is selected from hexanoic acid, heptanoic acid, decanoic acid, undecanoic acid, dodecanoic acid, tridecanoic acid, and tetradecanoic acid.
Assignees
Inventors
Classifications
Patent family
Related publications grouped by family.
External sources
Frequently asked questions
Answers are generated from the same data shown on this page.
- What does patent US12480147B2 cover?
- Provided herein are methods for making gamma lactones comprising reacting a carboxylic acid substrate with a heterologous cytochrome P450 (CYP450) protein with carboxylic acid 4-hydroxylase activity.
- Who is the assignee on this patent?
- Conagen Inc
- What technology area does this patent fall under?
- Primary CPC classification C12N15/52. Mapped technology areas include Chemistry & Metallurgy.
- When was this patent published?
- Publication date Tue Nov 25 2025 00:00:00 GMT+0000 (Coordinated Universal Time) (B2). Legal status and post-grant events are not shown on this page.
- What related patents are in patentsdb?
- We list 7 related publications on this page (citations in our corpus or others sharing the same primary CPC).