Plants having increased tolerance to herbicides

Patent metadata

Field	Value
Publication number	US-12480137-B2
Application number	US-202418642792-A
Country	US
Kind code	B2
Filing date	Apr 22, 2024
Priority date	Apr 23, 2014
Publication date	Nov 25, 2025
Grant date	Nov 25, 2025

Abstract

The present invention refers to a plant or plant part comprising a polynucleotide encoding a wildtype or mutated cellulose synthase (CESA) polypeptide, the expression of said polynucleotide confers to the plant or plant part tolerance to CESA-inhibiting herbicides, such as azines.

First claim

The invention claimed is:

1 . A plant or plant part comprising a polynucleotide encoding a mutated cellulose synthase (CESA) polypeptide, wherein expression of said polynucleotide confers to the plant or plant part tolerance to CESA-inhibiting herbicides, wherein the mutated CESA polypeptide comprises one or more of the following motifs:
i) Motif 1a:

(SEQ ID NO: 78)

[V/I][A/V]G[V/I/F][S/T][Y/D/N/A]A[V/I/L][N/S/G]

[S/N]G[Y/F/E][Q/D/G/E/H][S/A]WG[P/A]L[[F/M/L]G

[K/R][L/V][F/L]F,

wherein the amino acid at position 5 within said motif is substituted by any other amino acid than S or T;
wherein the amino acid at position 16 within said motif is substituted by any other amino acid; and/or
wherein the amino acid at position 17 within said motif is substituted by any other amino acid than P or A;
ii) Motif 2a:

(SEQ ID NO: 81)

[V/L/I]W[S/A][V/A/I]LL[A/S]S[I/F/V][F/L][S/T][L/V]

[L/M/V/I]WV[R/K][I/V][N/D]PF,

wherein the amino acid at position 8 within said motif is substituted by any other amino acid; and/or
the amino acid at position 11 is substituted by any other amino acid,
wherein the mutated CESA polypeptide comprises an amino acid sequence having, over the full-length, at least about 80% sequence identity to SEQ ID NO: 49 or 55, and
wherein the amino acid sequence of the mutated CESA polypeptide differs from the wildtype amino acid sequence at one or more positions corresponding to positions 1009, 1010, 1052, and 1055 of SEQ ID NO: 1.

2 . A seed comprising a polynucleotide encoding a mutated cellulose synthase (CESA) polypeptide, wherein the mutated CESA polypeptide comprises one or more of the following motifs:
Motif 1a:

(SEQ ID NO: 78)

[V/I][A/V]G[V/I/F][S/T][Y/D/N/A]A[V/I/L][N/S/G][S/

N]G[Y/F/E][Q/D/G/E/H][S/A]WG[P/A]L[F/M/L]G[K/R][L/

V][F/L]F,

wherein the amino acid at position 5 within said motif is substituted by any other amino acid than S or T;
wherein the amino acid at position 16 within said motif is substituted by any other amino acid; and/or
wherein the amino acid at position 17 within said motif is substituted by any other amino acid than P or A;
ii) Motif 2a:
[V/L/I]W [S/A] [V/A/I]LL [A/S]S [I/F/V] [F/L][S/T] [L/V] [L/M/V/I]WV [R/K] [IV] [N/D]PF (SEQ ID NO: 81),
wherein the amino acid at position 8 within said motif is substituted by any other amino acid; and/or
the amino acid at position 11 is substituted by any other amino acid,
wherein the mutated CESA polypeptide comprises an amino acid sequence having, over the full-length, at least about 80% sequence identity to SEQ ID NO: 49 or 55, and
wherein the amino acid sequence of the mutated CESA polypeptide differs from the wildtype amino acid sequence at one or more positions corresponding to positions 1009, 1010, 1052, and 1055 of SEQ ID NO: 1.

3 . A plant cell comprising a polynucleotide encoding a mutated cellulose synthase (CESA) polypeptide, wherein the mutated CESA polypeptide comprises one or more of the following motifs:
i) Motif 1a:

(SEQ ID NO: 78)

[V/I][A/V]G[V/I/F][S/T][Y/D/N/A]A[V/I/L][N/S/G][S/

N]G[Y/F/E][Q/D/G/E/H][S/A]WG[P/A]L[F/M/L]G[K/R][L/

V][F/L]F,

wherein the amino acid at position 5 within said motif is substituted by any other amino acid than S or T;
wherein the amino acid at position 16 within said motif is substituted by any other amino acid; and/or
wherein the amino acid at position 17 within said motif is substituted by any other amino acid than P or A;
ii) Motif 2a:

(SEQ ID NO: 81)

[V/L/I]W[S/A][V/A/I]LL[A/S]S[I/F/V][F/L][S/T][L/V]

[L/M/V/I]WV[R/K][I/V][N/D]PF,

wherein the amino acid at position 8 within said motif is substituted by any other amino acid; and/or
the amino acid at position 11 is substituted by any other amino acid,
wherein the mutated CESA polypeptide comprises an amino acid sequence having, over the full-length, at least about 80% sequence identity to SEQ ID NO: 49 or 55, and
wherein the amino acid sequence of the mutated CESA polypeptide differs from the wildtype amino acid sequence at one or more positions corresponding to positions 1009, 1010, 1052, and 1055 of SEQ ID NO: 1.

4 . A method for controlling weeds at a locus for growth of a plant, the method comprising: (a) applying an herbicide composition comprising CESA-inhibiting herbicides to the locus; and (b) planting a seed at the locus, wherein the seed is capable of producing a plant that comprises in at least some of its cells a polynucleotide operably linked to a promoter operable in plant cells, the promoter capable of expressing a wildtype or mutated CESA polypeptide encoded by the polynucleotide, the expression of the wildtype or mutated CESA polypeptide conferring to the plant tolerance to CESA-inhibiting herbicides, wherein the mutated CESA polypeptide comprises one or more of the following motifs:
i) Motif 1a:

(SEQ ID NO: 78

[V/I][A/V]G[V/I/F][S/T][Y/D/N/A]A[V/I/L][N/S/G]

[S/N]G[Y/F/E][Q/D/G/E/H][S/A]WG[P/A]L[[F/M/L]G

[K/R][L/V][F/L]F,

wherein the amino acid at position 5 within said motif is substituted by any other amino acid than S or T;
wherein the amino acid at position 16 with

Assignees

• Basf Se

Inventors

• Tresch Stefan
• Schachtschabel Doreen
• Sisay Mihiret Tekeste
• Lerchl Jens
• Major Julia
• Vogt Florian
• Calo Frederick
• Paulik Jill Marie

Classifications

• C12Y204/01012

  Cellulose synthase (UDP-forming) (2.4.1.12) · CPC title

• C12N9/1059

  Cellulose synthases (2.4.1.12; 2.4.1.29) · CPC title

• A01N43/68

  with two or three nitrogen atoms directly attached to ring carbon atoms · CPC title

• A01H4/008

  Methods for regeneration to complete plants · CPC title

• A01N43/66

  1,3,5-Triazines, not hydrogenated and not substituted at the ring nitrogen atoms · CPC title