Plant regulatory elements and uses thereof

Patent metadata

Field	Value
Publication number	US-12480136-B2
Application number	US-202418616128-A
Country	US
Kind code	B2
Filing date	Mar 25, 2024
Priority date	Apr 28, 2020
Publication date	Nov 25, 2025
Grant date	Nov 25, 2025

Abstract

The invention provides recombinant DNA molecules and constructs, as well as their nucleotide sequences, useful for modulating gene expression in plants. The invention also provides transgenic plants, plant cells, plant parts, and seeds comprising the recombinant DNA molecules operably linked to heterologous transcribable DNA molecules, as are methods of their use.

First claim

What is claimed is:

1 . A recombinant DNA molecule comprising a DNA sequence selected from the group consisting of:
a) a sequence comprising SEQ ID NO:5; and b) a fragment comprising at least 50 contiguous nucleotides of SEQ ID NO:5, wherein the fragment has gene-regulatory activity; wherein said sequence is operably linked to a heterologous transcribable DNA molecule.

2 . The recombinant DNA molecule of claim 1 , wherein said DNA sequence comprises a fragment comprising at least 75 contiguous nucleotides of SEQ ID NO:5, wherein the fragment has gene-regulatory activity.

3 . The recombinant DNA molecule of claim 1 , wherein said DNA sequence comprises a fragment comprising at least 95 contiguous nucleotides of SEQ ID NO:5, wherein the fragment has gene-regulatory activity.

4 . The recombinant DNA molecule of claim 1 , wherein the heterologous transcribable DNA molecule comprises a gene of agronomic interest.

5 . The recombinant DNA molecule of claim 4 , wherein the gene of agronomic interest confers herbicide tolerance in plants.

6 . The recombinant DNA molecule of claim 4 , wherein the gene of agronomic interest confers pest resistance in plants.

7 . The recombinant DNA molecule of claim 1 , wherein the heterologous transcribable DNA molecule encodes an dsRNA, an miRNA, or a siRNA.

8 . A transgenic plant cell comprising a recombinant DNA molecule comprising a sequence selected from the group consisting of:
a) a sequence comprising SEQ ID NO:5; and b) a fragment comprising at least 50 contiguous nucleotides of SEQ ID NO:5, wherein the fragment has gene-regulatory activity; wherein said sequence is operably linked to a heterologous transcribable DNA molecule.

9 . The transgenic plant cell of claim 8 , wherein said transgenic plant cell is a monocotyledonous plant cell.

10 . The transgenic plant cell of claim 8 , wherein said transgenic plant cell is a dicotyledonous plant cell.

11 . A transgenic plant, or part thereof, comprising the recombinant DNA molecule of claim 1 .

12 . A progeny plant of the transgenic plant of claim 11 , or a part thereof, wherein the progeny plant or part thereof comprises said recombinant DNA molecule.

13 . A transgenic seed, wherein the seed comprises the recombinant DNA molecule of claim 1 .

14 . A method of producing a commodity product comprising obtaining a transgenic plant or part thereof according to claim 11 and producing the commodity product therefrom.

15 . The method of claim 14 , wherein the commodity product is selected from the group consisting of seeds, processed seeds, protein concentrate, protein isolate, starch, grains, plant parts, seed oil, biomass, flour and meal.

16 . A method of expressing a transcribable DNA molecule comprising obtaining a transgenic plant according to claim 11 and cultivating the plant, wherein the transcribable DNA is expressed.

17 . The recombinant DNA molecule of claim 1 , wherein said DNA sequence comprises a sequence comprising SEQ ID NO:5.

18 . The recombinant DNA molecule of claim 1 , wherein said DNA sequence comprises a fragment comprising at least 50 contiguous nucleotides of SEQ ID NO:5, wherein the fragment has gene-regulatory activity.

Assignees

• Monsanto Technology Llc

Inventors

• Chittoor Jaishree M
• Flasinski Stanislaw

Classifications

• C12N2330/50

  Biochemical production, i.e. in a transformed host cell · CPC title

• C12N2320/50

  Methods for regulating/modulating their activity · CPC title

• C12N2310/141

  MicroRNAs, miRNAs · CPC title

• C12N15/8216 Primary

  Methods for controlling, regulating or enhancing expression of transgenes in plant cells · CPC title

• C12N2310/14

  interfering nucleic acids [NA] · CPC title