Plants with increased yield and method for producing said plants

Patent metadata

Field	Value
Publication number	US-12480134-B2
Application number	US-202117230404-A
Country	US
Kind code	B2
Filing date	Apr 14, 2021
Priority date	Feb 24, 2014
Publication date	Nov 25, 2025
Grant date	Nov 25, 2025

Abstract

The invention relates to a method for increasing the yield and biomass of a plant, by means of an increase in the expression of the L-aspartate oxidase in the plant. The method according to the invention allows an increase in the photosynthetic capacities of the plants as a result of an increase in the quantities of NAO and the derivatives thereof in said plants. The invention relates to the plants produced by such a method.

First claim

The invention claimed is:

1 . A method for improving at least one phenotypic trait of a plant, said method comprising transforming the plant with a nucleic acid sequence, wherein the nucleic acid sequence comprises a sequence encoding L-aspartate oxidase and comprises an exogenous promoter functionally linked to the sequence encoding L-aspartate oxidase,
wherein the at least one phenotypic trait is one or more selected from the group consisting of seed yield, abiotic stress resistance, biotic stress resistance, germination rate, and growth rate, wherein the abiotic stress resistance comprises at least one of intense sunlight, lack of light, excess water, cold, or excessive heat, wherein the nucleic acid sequence is present in a genome, wherein the resulting plant overexpresses L-aspartate oxidase, wherein the overexpression of L-aspartate oxidase leads to increased synthesis of NAD and derivatives thereof, and wherein said sequence comprises SEQ ID NO:1 or a sequence with at least 80% identity to SEQ ID NO:1, with the proviso that the sequence having at least 80% identity is not SEQ ID NO:15.

2 . The method according to claim 1 , further comprising cultivation of the plant to maturity, wherein the method improves the seed yield of the plant.

3 . The method according to claim 1 , further comprising cultivation of the plant to maturity, wherein the method improves the germination rate of the plant.

4 . The method according to claim 1 , further comprising cultivation of the plant to maturity, wherein the method improves the abiotic stress resistance and/or the biotic stress resistance of the plant.

5 . The method according to claim 1 , wherein the plant is selected from the group consisting of wheat, barley, rice, maize, sorghum, sunflower, rapeseed, soybean, cotton, pea, common bean, cassava, mango, banana, potato, tomato, pepper, melon, zucchini, watermelon, lettuce, cabbage, eggplant, and poplar.

6 . The method according to claim 1 , wherein the plant is rice ( Oryza sativa ), wheat, barley or maize.

7 . The method according to claim 1 , wherein the sequence encoding L-aspartate oxidase comprises a sequence having at least 90% homology with SEQ ID NO: 1.

8 . The method according to claim 1 , wherein the sequence encoding L-aspartate oxidase comprises a sequence having at least 95% homology with SEQ ID NO: 1.

9 . The method according to claim 1 , wherein the sequence encoding L-aspartate oxidase comprises a sequence having at least 98% homology with SEQ ID NO: 1.

Assignees

• Centre Nat Rech Scient
• Univ Paris Sud
• Univ Paris Saclay

Inventors

• De Bont Linda
• Gakiere Bertrand

Classifications

• C12N15/8286

  for insect resistance · CPC title

• C12N15/8279

  for biotic stress resistance, pathogen resistance, disease resistance · CPC title

• C12N15/8271

  for stress resistance, e.g. heavy metal resistance · CPC title

• C12N15/8267

  Seed dormancy, germination or sprouting · CPC title

• C12N9/0022

  with oxygen as acceptor (1.4.3) · CPC title